The effects of an aqueous extract of Dracocephalum tanguticum Maxim. (DtM ) on p, b.b-1/ H. Hct. ESR. ESR-kand EPT of rats were studied by ip route. The animals were divided into three groups: normal pressure control group (NCG ). iowpressure treatment group (LTG )and lowpressure control group (LCG ). A11 p,b. Het. ESR-k increased stignificantly in rats exposed to stimulated altitude of 6500m for 10 days (8h/day),and the platelet and the ratio of left to ri1ght ventricle weights were obviously decreased in comparison with NPG. The above parameters of LTG .except Het.have noobvious difference as compared with NCG, Het in LTG increased obviously but lower than LCG. These results suggested that DtM may be used as an inhibitory agent against the changes of induced-hypoxia blood rheology, decreased platelet and hypertrophic right ventricle. In addition,the observation of tissue morphology showed that DtM possesses sometherapeutic effects to injuries of lung. liver and kidney of hypoxia rats.

Objective To analyze the clinicopathologic features,prognosis and influencing factors of invasive cervical adenocarcinoma.Methods Clinical and pathologic data of 96 cases of invasive cervical adenocarcinoma and 100 cases of squamous carcinoma of the cervix treated in Shanxi Cancer Hospital from years of 1998 to 2007 were retrospectively analyzed.Results The morbidity of cervical adenocarcinoma was showing the arise and low-age trend.The overall 5-year survival rate was 60.4 ％,The overall survival rates of patients with stage Ⅰ,Ⅱ,Ⅲ,and Ⅳ were 82.9 ％,51.5 ％,13.3 ％ and 0,respectively.Ocervical adenocarcinoma had many prognosis-related factors:clinical stage,lymmphatic spread,vascular invasion,volume of tumor,histologic grade.Multivariate analysis showed that clinical stage,lymph nodal metastasis and vascular invasion were independent prognosis-related factors.However,whether ovary was reserved,it was irrelative to prognosis.Conclusion The morbidity of cervical adenocarcinoma is showing the arise and low-age trend.Earlier diagnosis is extremely important for improving the survival rate of cervical adenocarcinoma,the reserved ovary doesn't influence the overall 5-year survival rate for young women with early cervical adenocarcinoma.

From 1989 to Apr. 1997, 21 patients were admitted for gastrointestinal bleeding caused by binary angiodeformity. Diagnosis was made in 16 out of 18 undergoing selective celiac angiography with the occuracy rate of 88. 9%. Endoscopy found the deformity in 6 out of 14 (42. 8%). Bleeding stopped in 9 of 11 treated conservatively. Surgery was successful in all 9 cases,and endoscopic cautery successfully stopped the bleeding in the remaining one. The authors came to the conclusion that selective celiac angiography was most useful for the diagnosis. Drug therapy and endoscopic cautery effect only temporary hematostasis.

OBJECTIVE To explore the isolation,distributive characteristics and drug resistance of Acinetobacter baumannii producing AmpC enzyme in nosocomial infection.METHODS The distributive sections and infected parts of 42 A.baumannii strains in nosocomial infection were analyzed,and three dimensional test was used to(detect) AmpC enzyme.RESULTS Among them 18(42.86%) strains were AmpC positive.Among 40 strains of A.baumannii in non-nosocomial infection,6(15.00%) strains were AmpC positive.A.baumannii in nosocomial(infection) in clinical sections was mainly discovered in burn department,and the respiratory tract and skin soft tissue were the main infected sites(90%).The drug resistance in nosocomial infection was obviously higher than that of A.baumannii in non-nosocomial one.CONCLUSIONS The isolated rate and drug resistance rate of A.baumannii producing AmpC enzyme are rather high in nosocomial infection.It′s(neccessary) to take measures to prevent the nosocomial infection caused by A.baumannii.

Objective:In this study,a prokaryotic expression of the 3-ketosteroid-Delta (1)-dehydrogenase (KSDD) which came from Arthrobacter simplex was built.Moreover,in order to investigate the catalytic mechanism of KSDD and improve its stability,the structure of KSDD was predicted by computer and the critical sites were confirmed by site-directed mutations.Methods:The recombinant plasmid was constructed by eukaryotic expression vector pET-22b and the recombinant strain was constructed and expressed in Escherichia coli BL21 (DE3).High-performance liquid chromatography was used to determine the transformation rate of 4-AD to ADD.The KSDD structure and key sites were predicted by SWISS-MODEL.Site-directed mutations for the amino acid residues of key sites were constructed and activities of the mutations were detected.Results:The recombinant strain E.coli pET-22-ksdd was successfully constructed.It was induced to express the dehydrogenase by IPTG and the conversion rate of 4-AD to ADD was 27％ at 21 ℃.The structure of 3-ketosteroid-Delta (1)-dehydrogenase and the four key sites was analyzed by SWISS-MODEL.Four mutants,Y120R,Y320L,Y488F and G492Y were constructed.Mutants Y120R and Y488F were inactivated,so they were proved to be the key active sites of KSDD.The conversion rate of mutant Y320L was consistent with that of wild type,but the stability at 37 ℃ was improved.The conversion rate of mutant G492Y was 1.2 times of the wild type and the stability has been improved at 37 ℃.Conclusions:At present,there are few studies about the structure and catalytic mechanism of dehydrogenase.The active sites of the enzyme were verified by this study,which laid the foundation for the further study of the properties of the enzyme KSDD.

Objective To study the preparation of nifedipine liposomes (NP) and evaluate their quality. Methods The liposomes were prepared by a pH steps degree-freeze thawing method, with the entrapment and stability as indices. The entrapment was studied by Sephadex G-50 minicolumn centrifugation UV-Vis spectroscopy method. Entrapment, and degree distribution before and after freezing were compared, the effect of freezing times on entrapment was studied. Results The entrapment and the average size of NP liposomes were 96.65% and 2.0 ?m, respectively. After freezing, both the entrapment and stability were increased. Conclusion The selected formulation and preparation of nifedipine liposomes are practicable.

Objective To study the effects of several enhancers on transdermal permeation of berberine hydrochloride. Methods The penetration rate of berberine hydrochloride through rat skin in 30% ethanol-water was measured using the penetration experiment apparatus. Samples were withdrawn from the receptor solution at certain time intervals and assayed by HPLC method. Results The penetration rate of berberine hydrochloride by the enhancers was increased in the following order: azone

Acute hepatic failure was induced in rats with intraperitoneal administration of D-galactosamine.Serum and tissue amino acid profiles of the animals were studied. Except for arginine, serum levels of all amino acids were elevated. This is in variance with the findings of Fischer and other investigators. In their studies, the decreased levels of BCAA could be related to the administration of dextrose. In our study, the serum BCAA/AAA ratio was decreased and it was correlated negatively with the dosage of D-galactosamine (r =-0.789). Amino acid profiles in the liver,kidney and muscles were significantly altered. The chracteristics of the changes indicated that enhanced proteolysis of tissue proteins was the major factor accounting for the elevation of serum amino acid levels. Hepatic coma might be related to the increase of aromatic amino acids in the brain. It is usually difficult for glycine, cystine, asparagine and threonine to penetrate the blood-brain-barrier. Elevation of their levels in the brain might be a consequence of increased permeability of the blood-brain-barrier. Further studies are needed to clarify the role of GABA in hepatic coma.

Objective:To explore the impact of clinical pharmacists involved in the treatment team on the reasonable drug use rate in medical orders. Methods:Totally 782 medical records including hypertension and 1-3 kinds of hypertension associated diseases in our hospital from January 2011 to December 2011 were used as the pre-intervention group, and 1 061 ones with the same conditions from September 2012 to August 2013 were used as the post-intervention group. The patients of the two groups were divided into 8 dis-ease subgroups, clinical pharmacists performed timely intervention on the medical orders in the post-intervention group, and the rational drug use rate between the two groups was compared. Results:In hypertension, hypertension associated coronary heart disease, hyper-tension with heart failure and hypertension with diabetes subgroups,the difference in the rational drug use proportion before and after the intervention was statistically significant(P<0. 01), in hypertension with coronary heart disease and heart failure, hypertension associ-ated coronary heart disease and diabetes, hypertension with heart failure and diabetes subgroup, the difference in the rational drug use proportion before and after the intervention was statistically significant(P<0. 05), while in hypertension with coronary heart disease, heart failure and diabetes subgroups, the difference was not statistically significant(P>0. 05). After the medical records in the 8 sub-groups were aggregated, the difference in the rational drug use proportion before and after the intervention was statistically significant( P<0. 01). Conclusion:Clinical pharmacists should involve in the clinical treatment to intervene irrational drug use,which can signifi-cantly improve the medical record rationality, avoid the health risks as much as possible and ensure the medication safety and treatment effectiveness for patients.

Objective:To prepare and evaluate Shenhuang vaginal thermosensitive in situ gels. Methods:Shenhuang vaginal ther-mosensitive in situ gels( SHV-TISG)were prepared with poloxamer 407 and poloxamer 188 as the materials. The viscosity change at different temperatures and the gelation temperature of SHV-TISG and SHV-TISG diluted by stimulant vaginal fluid were measured,the in vitro drug release of the preparation was evaluated by a membraneless diffusion method,and the safety of SHV-TISG was detected by vaginal irritation test. Results:The preparation showed the vaginal injectability and gelationability in vivo without vaginal irritation. Gel erosion was the principal mechanism of the drug release from the gels. Conclusion:The preparation of SHV-TISG is feasible in tech-nique,and the properties meet the requirements of vaginal topical use.