1.Application of reverse engineering to reconstruction of dental models
Jianzhi CHEN ; Hongyan PENG ; Guilan SUN
Chinese Journal of Medical Aesthetics and Cosmetology 2001;0(04):-
Objective To discuss the foreground of application of reverse engineering reconstruction of dental models and to establish the foundation of developing a system for complete denture CAD/CAM. Methods The models of an edentulous jaw were measured with reverse engineering, and the 3D models were reconstructed with a computer. Results 3D models of the edentulous jaw were achieved. The characteristics of these 3D images were clear and accurate. Conclusion CAD models are reconstructed with reverse engineering accurately and technical problems in 3D model of complete denture CAD/CAM are resolved.
2.The clinical and pathological aspects of digestive tract bleeding caused by angiodeformity of the bile duct:report of 21 cases
Guilan ZHAO ; Shuyong PENG ; Fan ZHOU
Chinese Journal of General Surgery 1994;0(05):-
From 1989 to Apr. 1997, 21 patients were admitted for gastrointestinal bleeding caused by binary angiodeformity. Diagnosis was made in 16 out of 18 undergoing selective celiac angiography with the occuracy rate of 88. 9%. Endoscopy found the deformity in 6 out of 14 (42. 8%). Bleeding stopped in 9 of 11 treated conservatively. Surgery was successful in all 9 cases,and endoscopic cautery successfully stopped the bleeding in the remaining one. The authors came to the conclusion that selective celiac angiography was most useful for the diagnosis. Drug therapy and endoscopic cautery effect only temporary hematostasis.
3.The Bilingual Teaching Materials with Innovation and Construction
Li CHEN ; Guilan WANG ; Xiaolei CAO ; Yufeng XIAO ; Peng LU
Chinese Journal of Medical Education Research 2006;0(09):-
Objective:to construct and apply the bilingual teaching materials.Methods:teachers in Nantong university compiled Pathology,Direction of pathological experiment and test which were written in both Chinese and English as the in-class bilingual teaching materials according to the characteristics of Chinese students,meanwhile compiled Pathological formative exercises,Pathological listening and reading,Basic problems of modern clinical pathological research for students' practice and elective course materials or reading materials after class.Results:A series of 4 bilingual teaching materials were all published by science publishing house except Pathological listening and reading was used as in class teaching materials.These teaching materials played an important role in the bilingual pathological teaching in Nantong university.Conclusion:The construction and innovation of bilingual teaching materials are basic guarantees for bilingual teaching.
4.Reform and Innovation of Pathology Teaching in Nantong University
Li CHEN ; Guilan WANG ; Xiaolei CAO ; Peng LU
Chinese Journal of Medical Education Research 2003;0(03):-
Objective:To cultivate the students' learning ability and innovation spirit to better grasp the pathological knowledge,the Pathology Department of Nantong University carried out all-round research and practice of education reform in many links and many stages.Methods:To establish and perfect the teaching syllabus scientifically,combine class teaching with the second classroom activity as well as innovate examination mode and evaluation system.Results:The syllabus design not only fulfills the need of talent training,fits the actual level of the students,but also embodies the principle of the combining of imparting knowledge,fostering ability and raising quality.In addition,it also reflects the characteristics of pathology itself.Opening the second classroom activities of pathology is beneficial complementary to the course content.Various examination forms are used to have a comprehensive assessment of the students,creating an equal and fair competitive environment and competition,learning,exceeding atmosphere.Conclusion:The reform and innovation of pathological teaching of Nantong University can mobilize the students to learn with many sense organs all-directionally,match the cognition rules,promote the students' comprehension,apprehension and application about pathological knowledge/skills and embody the students' dominant position in teaching,which is warmly accepted by students.
5.The assessment and analysis of nutritional status of 1200 inhospital patients
Ping SHEN ; Zhiying ZOU ; Nanhai PENG ; Guilan LU ; Jihong ZHONG ; Linfeng HU ; Yahong LIU ; Gui ZHANG
Parenteral & Enteral Nutrition 1997;0(01):-
Objective:To assess incidence of malnutrition and malnutrition risk of six department patients.Methods:The information of 1 200 patients were collected,200 in each of 6 departments in our hospital.Nutrition status was assessed according to Nutrition Risk Screening(NRS)published by ESPEN in 2001.Results:The incidence of malnutrition and malnutrition risk varied from 7.5% to 59% and 36% to 72% respectively in different department.Conclusion:The incidence of malnutrition is closely related to the kind and severity of the disease.It is nessissary to assess the nutrition status of high risk patients in time.NRS can be used simply and fastly in most inhospital patients.
6.Application and Discussion of Mind Mapping in Teaching of Rehabilitation
Guilan HUANG ; Yongmei PENG ; Hong ZHANG ; Ming XU ; Shuai LI ; Jie TAN ; Jiaqing LIU ; Tongyan LIU
Chinese Journal of Rehabilitation Theory and Practice 2017;23(8):989-992
As a new learning tool, mind mapping helps students master the learning content in a visual and radical way. This paper intro-duced the concept and method of making the mind map and analyzed the advantages of mind mapping in improving learning efficiency and its application in rehabilitation. It is pointed out that the use of mind mapping would help students to integrate new and old knowledge, to improve the ability to solve clinical problems, and to train innovative talents by stimulating students' divergent thinking. It is believed that the mind map has a broad application prospect in rehabilitation education in China.
7.Whole-genome analysis for 32 influenza A(H3N2) viruses in severe acute respiratory infection cases
Yang PAN ; Yi ZHANG ; Peng YANG ; Weixian SHI ; Xiaomin PENG ; Shujuan CUI ; Daitao ZHANG ; Guilan LU ; Jiachen ZHAO ; Quanyi WANG
International Journal of Laboratory Medicine 2017;38(17):2367-2370
Objective To investigate the genetic characteristic of whole-genome of influenza A/H3N2 viruses in severe acute respiratory infection cases in Beijing area.Methods From 2014 to 2016,the viral RNA was extracted from 32 strains isolated from SARI cases,then sequenced by Ion Torrent PGM Sequencer.The phylogeny and molecular features of whole-genome were analyzed by Mega and Consurf software.Results The HA gene of tested strains isolated in 2014-2015 influenza season belonged to lineage 3C.3a and 3C.2a,while those isolated in 2015-2016 influenza season belonged to cluster 3C.2a.Moreover,compared with the vaccine strains,7 variant amino acids of protein of HA1 were identified,and two of them were located in antigenic sites.All isolates were sensitive to neuraminidase inhibitors while showed resistance to blockers for M2 ion channel.Conclusion The phylogenetic features of isolates studied in this study are similar with that of current circulating strains.However,the difference between isolates and vaccines should not be overlooked.
8.Study on the ELISA Double-antibody Sandwich Method and Pharmacokinetics of Recombinant Human Calcineurin B Subunit in Rat Plasma
Kesheng HAN ; Xueli XIE ; Shuhong TIAN ; Guilan XING ; Jiang FU ; Jun XING ; Chunhua LIN ; Peng TAN ; Jiping SHAO
China Pharmacy 2016;27(25):3468-3470
OBJECTIVE:To establish a method for determining recombinant human calmodulin B subunit(rhCNB)in rat plas-ma,and study its pharmacokinetics characteristics. METHODS:ELISA double-antibody sandwich method was adopted. 1 μg/ml rhCNB monoclonal antibody mAb was wrapped,added to the to-be-test sample,rhCNB polyclonal antibody pAb(dilution ratio of 1∶5 000)and HRP-labeled conjugate of anti-IgG(dilution ratio of 1∶10 000)were added. Using tetramethylbenzidine for develop-ing,microplate reader was conducted in wavelength of 450 nm to determine the absorbance value(OD value)and plasma concen-tration of 6 rats after 2,15,30,60,120,240,480,720 min of iv 2.5 mg/kg rhCNB,and the pharmacokinetic parameters were calculated by BAPP 3.0 software. RESULTS:The linear range of rhCNB were 0.195-12.5 ng/ml(r2=0.995 0),lower limit of quan-titation was 0.195 ng/ml,accuracy were 97.300%-103.622%(RSD<7.5%,n=6);RSDs of within-batch,inter-batch,freezing and thawing 3 times were no higher than 8.5%(n=6,18,15). rhCNB pharmacokinetics characteristics in rat fitted to two-com-partment model,AUC0-720 min was 173.038 mg·min/L and t1/2 was 94.62 min. CONCLUSIONS:The established method has high specificity and sensitivity,good accuracy and precision,which can be used for rhCNB quantitative detection and pharmacokinetics study in biological samples.
9.Study on the relationship between M protein gene-types and superantigen genes of Group A Streptococcus pyogenes strains isolated from scarlet fever patients in Beijing
Shuangsheng WU ; Xiaomin PENG ; Chunna MA ; Daitao ZHANG ; Guilan LU ; Yang PAN ; Shujuan CUI ; Weixian SHI ; Yimen LIU ; Jiachen ZHAO ; Quanyi WANG ; Peng YANG
Chinese Journal of Infectious Diseases 2015;(10):611-614
Objective To explore the relationship between superantigen and M protein gene (emm)-types genes of Group A Streptococcus pyogenes (GAS) isolated from patients with scarlet fever in Beijing from May 2012 to July 2013 .Methods GAS was isolated from specimens of patients with scarlet fever . Superantigen genes (speA ,speB ,speC ,speF ,speG ,speH ,speI ,speJ ,speL ,speK ,speM ,ssa ,and smeZ) ,and emm gene were amplified by polymerase chain reaction .Rate and proportion were compared by chi-square test .Results Of the 423 GAS strains isolated from patients with scarlet fever from 2012 to 2013 ,most of the isolates possessed speB (97 .6% ) ,speC (99 .8% ) ,speF (98 .3% ) ,speG (99 .8% ) , smeZ (94 .1% ) and ssa (88 .4% ) ,and some of them possessed speH (54 .6% ) ,speI (53 .4% ) ,speA (45 .2% ) and speJ (43 .5% ) ,but very few isolates possessed speK (2 .4% ) ,speL (1 .4% ) and speM (1 .7% ) .Type emm12 (59 .5% ) and type emm1 (37 .4% ) were the main types of GAS .Most of the emm12-type isolates possessed speH (84 .8% ) and speI (84 .0% ) compared with only 4 .0% of speH and 3 .4% of speI in type emm1 .Most of type emm1 possessed speA (95 .3% ) and speJ gene (94 .6% ) compared with only 17 .3% of speA and 14 .8% of speJ in type emm12 .The superantigen genes profiles were significant different between emm 1-type and emm 12-type isolates (P< 0 .05) .Conclusion Type emm1 and type emm12 are epidemic strains in patients with scarlet fever from 2012 to 2013 in Beijing ,and emm gene-types are associated with superantigen genes profiles .
10.Clinical application of real-time PCR for the detection of genetic mutations underlying spinal muscular atrophy.
Yu JIANG ; Guilan PENG ; Qichang WU ; Yulin ZHOU
Chinese Journal of Medical Genetics 2014;31(2):180-184
OBJECTIVETo verify the reliability of real-time PCR for the detection of genetic mutations underlying spinal muscular atrophy (SMA) and establish quality control for clinical testing.
METHODSThirty-five patients, 61 first-degree relatives, 61 healthy controls and 7 prenatal cases which were previously genotyped by multiplex ligation-dependent probe amplification (MLPA) were tested with Roche LightCycler 480 and Bio-Rad CFX96 (TM) real-time PCR machines for relative quantification of copy number of SMN1 exon 7.
RESULTSGenotyping detected by relative quantitative real-time PCR were consistent with the results of MLPA. Both types of real-time PCR machines could accurately distinguish different SMN1 copy numbers despite certain systematic differences between the two platforms.
CONCLUSIONThe reliability of real-time PCR assay for detecting SMA depends on quality control. Standard database generated with known SMN1 copy number variations should be established for different instruments.
Gene Dosage ; Humans ; Muscular Atrophy, Spinal ; genetics ; Mutation ; Real-Time Polymerase Chain Reaction ; methods ; Survival of Motor Neuron 1 Protein ; genetics