Objective To analyze the effects of annexin Ⅱ antisense expression vector on the growth of the transplanted tumor in nude mice. Methods The SPC-A-1 cells (parental group) and SPC-A-1-annexin Ⅱ cells (antisense group) were inoculated subcutaneously in nude mice respectively. The forming time, volume, and weight of tumor were measured. The tumor cells and the surrounding tissues were observed microscopically. Results The forming time of tumor in the antisense group was later than that in the parental group. The volume and weight of tumor in the antisense group were lower than those in the parental group. Furthermore, invasion of tumor in the surrounding tissues was found in 2 cases in the parental group, but not in the antisense group. Conclusion The annexin Ⅱ gene may be important in promoting tumor cell growth and invading the surrounding tissues.

Objective To explore the effects of antisense vector of annexinⅡ gene on the growth of SPC A 1, a human lung cancer cell line. Methods The total RNA was isolated from human lung cancer cell line SPC A 1 and the target DNA fragments were amplified by RT PCR. The antisense expression vector was constructed by double restriction endonuclease cleavage directional clone method. Annexin Ⅱ antisense expression vector was introduced into SPC A 1 cells by liposome transfection reagent. The expression of annexin Ⅱ mRNA was analyzed by semi quantitative RT PCR. The effects of antisense vector of annexinⅡ gene on the growth of SPC A 1 were observed. Results The antisense vector of annexinⅡ gene was constructed and introduced into SPC A 1 cells successfully. Semi quantitative RT PCR showed that the annexin Ⅱ mRNA expression reduced by about two thirds in the transfected cells as compared with that in the untransfected cells. Compared with the untransfected cells, transfected cells decreased significantly in cell growth, clone formation efficiency in plating and DNA synthesis. Cell cycle was blocked in G 0 G 1 phase. Conclusion Annexin Ⅱ could promote the growth of lung cancer cells and may be helpful for the development of lung cancer.

OBJECTIVETo study the clinical characteristics, diagnosis and surgical treatment of malignant intracranial teratomas.

METHODSThirty-four patients with intracranial teratoma proved by histopathology were treated by operation. The growth pattern of this tumor, assessed by its clinical manifestations and neuroimaging together with surgical treatment and results were analyzed retrospectively.

RESULTSOnly 6 lesions had been correctly suspected as teratoma before surgery. Total removal was achieved in 14 patients with a operative mortality of 32.4%. The survival of 23 patients with lesions showing aggressive growth was significantly different from those without (P < 0.05). Nineteen of these patients did not survive beyond the sixth month after surgery.

CONCLUSIONAccurate preoperative diagnosis is difficult in malignant intracranial teratoma, especially for patients with the tumor in the sella region. The invasive biological behavior of the tumor is proved to be the main cause of surgery being dwarfed. Protection of the hypothalamus and brainstem, relief of hydrocephalus are the crucial points in surgical treatment. Comprehensive histopathologic examination combined with serum and CSF tumor marker detection is necessary for correct diagnosis and treatment.

Adolescent ; Adult ; Biomarkers, Tumor ; blood ; cerebrospinal fluid ; Brain Neoplasms ; diagnosis ; mortality ; pathology ; surgery ; Child ; Female ; Humans ; Male ; Neoplasm Invasiveness ; Prognosis ; Teratoma ; diagnosis ; mortality ; pathology ; surgery

Objective To observe the application of the mini-mental status examination (MMSE)cognitive assessment in the patients with brain glioma before and after surgery. Methods Using MMSE,36 pastients with primary brain glioma were subjected to the cognitive assessment before surgery, after surgery,and 3 monthsr after surgery. Results The quantitative cognitive assessments with MMSE before surgery revealed the hidden cognitive dysfunction patients.The quantitative cognitive assessments after surgery showed that surgeons might need to protect the non-function area and to form the idea of cognitive function in patients with glioma.Conclusion MMSE assessment is a simple,understandably,and convenient method having good compliance of patient. It may be effectively used to assess cognitive impairment for patients with glioma and worth being studied continuously and used widely in the clinic practice.

Objective:To compare the efficacy and safety of suture tape augmentation versus modified Brostr?m repair in the treatment of chronic lateral ankle instability.Methods:CNKI, Wanfang, Chinese Medical Journal full-text Database, PubMed, and Web of Science databases were searched for clinical studies comparing suture tape augmentation and modified Brostrom repair in the treatment of chronic lateral ankle instability. Meta-analysis was performed on the included studies. Fixed-effects models were used for analyses when heterogeneity between groups was small, and random-effects models were used when heterogeneity between groups was large. The quality of literature was assessed using the Modified Jadad Rating Scale for randomized controlled trials and the Newcastle-Ottawa Scale (NOS) for non-randomized controlled trials.Results:A total of 11 articles were included in the meta-analysis. There were 398 cases in the suture tape augmentation group and 404 cases in the modified Brostrom repair group. Meta-analysis showed no statistically significant differences between the two groups in postoperative pain visual analogue scores [ SMD=-0.19, 95% CI(-0.54, 0.16), P=0.282], talar tilt angle [ SMD=-0.37, 95% CI(-0.84, 0.11), P=0.128], and anterior displacement of the talus [ SMD=-0.61, 95% CI(-1.47, 0.24), P=0.220]. In the suture tape augmentation group, foot and ankle ability measure-daily living score[ SMD=0.70, 95% CI(0.01, 1.39), P=0.045], foot and ankle ability measure-movement score [ SMD=0.96, 95% CI(0.15, 1.77), P=0.020], foot and ankle outcome score-daily living score [ SMD=1.21, 95% CI(0.05, 2.36), P=0.040], foot and ankle outcome score-movement score [ SMD=1.60, 95% CI(0.11, 3.09), P=0.035] were greater than those in the modified Brostr?m repair group, and the incidence of recurrent postoperative instability was lower than that in the modified Brostr?m repair group [ RR=0.34, 95% CI(0.15, 0.76), P=0.008], the difference was statistically significant. Conclusion:Suture tape augmentation provides superior postoperative daily activity capacity and sports function compared to modified Brostr?m repair in the treatment of chronic lateral ankle instability. Additionally, the incidence of recurrent instability is lower in the suture tape augmentation group. No significant differences were observed between the two groups regarding to pain relief and radiological outcomes.