Objective:To study the expression of IP-10 and its mRNA in liver biopsy of patients with cirrhosis.Methods:The typical patients with cirrhosis after HBV infection were selected,and their serum IP-10 concentrations were evaluated with ELISA,the content of IP-10 mRNA in liver biopsy was measured by Real-time PCR.The level of IP-10 mRNA was represented by lg cDNA/lg GAPDH.The expression of IP-10 in liver biopsy tissue was detected by SP immunohistochemistry stain.Results:The levels of IP-10 in serum and its mRNA in liver biopsy of patients with cirrhosis were (299.3?77.2)pg/ml and (0.856 3?0.150 6),lg cDNA/lg GAPDH respectively,which were significantly higher than those of control groups(P

Objective To study the content of IL-8 in the peripheral blood and the expression of its mRNA in liver biopsy tissue of patients with hepatocirrhosis.Methods The patients with typical hepatocirrhosis resulted from HBV infection were selected.The IL-8 concentrations in peripheral blood of the Datients were evaluated with ELISA.The content of IL-8 mRNA in liver biopsy was measured by real-time PCR.The level of IL-8 mRNA was surrogated by lg cDNA/lg GAPDH.Reslllts The levels of IL-8 in the serum and its mRNA in hepatic tissue of patients with hepatoeirrhosis were(43 1.39±97.39)μg/L and 1.3647±0.2203,respectively,which were significantly higher than those of control group.MoreoCer,the levels of IL-8 in the serum and IL-8 mRNA in hepatic tissue of patients with positive HBV-DNA were (502.43-4-102.24)μg/L and 1.51424±0.2245,respectively,and both ofwhich were higher than those of patients without HBV-DNA replication(P<0.0l,P<0.05).And the levels of IL-8 in the selqlm and its mRNA in hepatic tissue of patients with higher ALT.AST also increased significantly.compared with those in control group(P<0.01).Conclusion The levels of IL-8 in the peripheral blood and the IL-8 mRNA in liver biopsy tissue of patients with hepatocirrhosis increase.which are related to the degree of HBV-DNA replication and the damage of hepatocytes.The IL-8 plays a key role in local inflammatory infihration of liver and the progression of post-hepatitis cirrhosis.

Objective To investigate the clinical application effects of atrial electrocardiogram guided the three directional valve PICC tip localization method. Methods After application of instant location technology of arterial electronic monitoring in the experimental group, the patients received X ray examination on chest, and double check the top catheter location of PICC;the control group utilized routine location method to ensure the top catheter position, which inserted the fitted length of tube ( from the puncture position to right chest lock joint then to the third intercostal space) then sent to do X ray examination to ensure the top catheter position. The incidences of location accuracy, operation time and cost were compared between two groups. Results The accuracy of locations were 98. 7% in the experimental group and 91. 0% in the control group (χ2 =8. 01,P<0. 05);the operation time was (16. 29 ± 0. 35) min in the experimental group compared with (13. 58 ± 0. 52) min in the control group (t=7. 12,P<0. 05);the cost was (2 364. 00 ± 0. 52) and (2 375. 00 ± 0. 35) in the experimental and control group (t=0. 98,P>0. 05). Conclusions The position control of atrial electrocardiogram technology was applied to place PICC, which can judge catheter top position promptly and accurately, improve the accuracy rate of PICC catheter positioning effectively, and increase work efficiency.