MicroRNA(miRNA)is a kind of 22 nt endogenous small non-coding single strand RNA and is universally present in animals and plants.It can suppress the expression of target genes at post-transcription al level by complementary biding to the 3'UTR of target mRNAs.Recent studies indicate that microRNA is closely associated with tumorigenesis,differentiation,metastasis and relapse.MicroRNA might act as a new biomarker of tumor diagnosis and a new target of tumor gene therapy.

Objective To investigate cancer suppressor Genes p16, p15 in the brain gliomas expression and its relations with the gliomas pathology graduation and the malignant degree.Methods P16 and P15 protein expression level in human brain gliomas from 63 surgery specimens were assayed by immunobistochemical S-P method.Results P16, the P15 protein expression rate the brain gliomas pathology graduation assumes the inverse correlation with, also compared with the high malignant group the low malignant group (Ⅰ~Ⅱ level) (Ⅲ~Ⅳ level) has the remarkable difference (P

0.05). Conclusions The expression of p16、 C-erbB-2 protein is correlated with gastric cancer and p16、C-erbB-2 protein can be used to evaluate the biological behavior and prognosis of gastric cancer.

Objective To establish an appropriate cut-off value of high sensitivity cardiac troponin T (hs-cTnT) and optimal combination measurement in the early diagnosis of acute myocardial infarction (AMI).Methods This research is a prospective study.342 patients admitted to emergency department with chest pain,43 patients with renal failure,40 patients with pneumonia and 18 premature with patent ductus arteriosus were involved from June 2012 to June 2013 in Peking University Third Hospital.The plasma hs-TnT,NT-proBNP,cardiac troponin Ⅰ (cTnI),CK-MB and copeptin were measured.The distribution of hs-cTnT among associated diseases was analyzed,the diagnostic performance of hs-cTnT and the role of combination hs-cTnT with NT-proBNP,CK-MB and copeptin were evaluated by receiver operating characteristic (ROC) curve.The statistical method was used to calculate the Sensitivity,specificity,negative predictive value and positive predictive value of hs-cTnT in the diagnosis of AMI.Results As compared to patients with STEMI(median 0.52 μg/L,range 0.037-7.610 μg/L),hs-cTnT was lower in the patients with Non-STEMI(median 0.127 5 μg/L,range 0.021-4.260 μg/L).However,the levels of hs-TnT in other diseases were also increased increased in varyng degrees (Chi-square =76.432,P ＜ 0.05)The areas under the curve (AUC) for hs-cTnT and cTnI in the diagnosis of AMI were 0.862 (95％ CI:0.729-0.928) and 0.748 (95％ CI0.666-0.818) respectively (Z =2.713,P ＜ 0.05).Taking 0.014μg/L and 0.035 μg/L as cut-off value of hs-TNT,the sensitivities were 100％ vs 95.1％,the specificities were 44.4％ vs 65.7％.The combination of hs-cTnT,NT-proBNP,CK-MB resulted in a increase in AUC (0.915,95％ CI:0.838-0.964) (Z =2.147,P ＜ 0.05) and the combination of hs-cTnT and copeptin resulted in a increase in AUC 0.921 (95％ CI:0.820-0.975) (Z =2.589,P ＜ 0.05).Conclusion With the cut-off value of 0.035 μg/L for diagnosis of AMI was appropriate,and the combination measurement can improve the accuracy of early diagnosis of AMI.

This study was aimed to optimize the best extraction technology of traditional Chinese medicine (TCM) Biminkang and establish the HPLC-ELSD method for determination of Astragaloside Ⅳ content. This test used heat-ing reflux which preferred ethanol as solvent extraction, extraction rate as an index to extract. By the single factor ex-periment, three factors which affect extraction rate greater were selected from the solvent concentration, extraction time, liquid ratio and extraction times. And then L9(34) orthogonal test was used to design the extraction technology of compound preparation Biminkang. HPLC-ELSD was performed on Diamonsil C18 column (250 mm í 4.6 mm, 5 μm) with H2O(A)-acetonitrile(B) (0~45 min: 22%B, 45~60 min: 22%~32%B) as mobile phase, flow rate was at 1.0 mL·min-1. The temperature of drift tube was 100℃ and the flow rate of N2 was 2.5 L·min-1. The column temperature was 30℃. The results showed that the best extraction technology of compound preparation Biminkang was liquid-solid ra-tio of 8 mL·g-1, ethanol concentration of 70%, 1.5 h for each extraction time, and extracted for three times. The re-sults showed that the presence of ethanol concentration and extraction times affected significantly. The ultimately de-termined optimal extraction conditions were as follows. The liquid-solid ratio of 8 mL/g, ethanol concentration of 70%, 1.5 h for each extraction time, and extracted for two times. The linear range of Astragaloside Ⅳ content was from 0.87 μg to 8.72 μg. And the regression equation was Y = 1.545 4X + 5.875 9, r = 0.999 7. The average re-covery rate was 95.05%. The RSD was 2.64%. It was concluded that the optimized extraction technology was stable, reasonably practicable, and suitable for industrial production.

Objective To investigate the impact of overweight and obesity on left atrial (LA) function in healthy subjects with excess body weight.Methods Conventional echocardiography and tissue Doppler imaging were performed in 30 obese subjects (BMI≥28 kg/m2),30 overweight subjects (BMI,24to 28 kg/m2) and 30 age-matched normal subjects (BMI＜24kg/m2).Strain (S),peak systolic strain rate (SSR),peak early diastolic strain rate (ESR) and peak late diastolic strain rate (ASR) values were used to evaluating LA function.Results Compared with controls,mean S,SSR and ESR were decreased in obese subjects,while mean SSR,ESR and ASR were decreased in overweight subjects.Compared with overweight subjects,mean ESR was decreased in obese subjects.Conclusions An impaired LA function is found in overweight and obese subjects who has no other clinically appreciable cause of heart disease by using strain and SR imaging.

Objective To evaluate the efficacy of TCM Bufei Yishen method combined with conventional western medicine for patients with stable chronic obstructive pulmonary disease (COPD) presenting lung-kidney deficiency.Methods Sixty six COPD patients with lung-kidney deficiency admitted from January 2014 to December 2015 were randomly divided into Bufei Yishen (BY) group (n=34) and control group (n=32).Patients in control group treated with conventional western medicine and those in BY group were treated with TCM Bufei Yishen recipe combined with conventional western medicine.The pulmonary function and COPD Assessment Test (CAT) score were evaluated before and after treatment,and after 3 month-following up.Results In BY group the pulmonary function parameters FEV1 and FEV1/FVC after treatment were significantly higher than those before treatment (69.91±8.15 vs.51.56 ± 8.33,t=4.435,P< 0.01;57.33 ± 9.86 vs.49.89 ± 8.89,t=3.526,P<0.05,respectively);the FEV1 and FEV1/FVC after 3-momth follow up were significantly higher than those before treatment (65.68±9.54 vs.51.56±8.33,t=3.993,P<0.05;59.08±8.49 vs.49.89±8.89,t=3.668,P<0.05,respectively).In control group the FEV1 and FEV1/FVC after 3-momth follow up were significantly higher than those before treatment (63.94±8.76 vs.50.98±8.08,t=4.475,P<0.05;59.12±9.55 vs.50.35±9.06,t=3.614,P<0.05,respectively).The pulmonary function parameters FEV1 of BY group was significantly better than that of control group (69.91±8.15 vs.62.02±8.91,t=3.745,P<0.05) after 3-months of follow-up.After treatment,the CAT score in both group was decreased (BY group: 27.35±2.93 vs.30.36±3.19,t=3.548,P<0.05;control group: 29.06±2.67 vs.30.51±2.99,t=1.832,P<0.05);however,the CAT score was decreased more markedly in BY group than that in control group (t=2.658,P<0.05).Conclusion TCM Bufei Yishen method combined with conventional western medicine can improve the pulmonary function and quality of life of patients with stable COPD presenting lung-kidney deficiency.

Objective By reviewing the relevant literature, we explored the current status and problems of the evaluation of the training effectiveness, and put forward the corresponding countermeasures, in order to provide a theoretical basis for the effect evaluation of nurse training in China. Methods By searching Citation online search and integration system (CITE), the Chinese biomedical literature service system (CBM), we included the literatures on the effect evaluation of the nurses training in the core journals from January 2006 to November 2015. Retrieval literatures were imported the bibliographic management software Note express by database. The corresponding field were analyzed in the literature′s age distribution, evaluation object, evaluation model, evaluation method, evaluation index and evaluation tool, et al. Results There were 343 literatures had retrieved, the total number of literatures presented a increasing tendency. The evaluation methods, evaluation tools and evaluation indicators presented a tendency of diversity, and the evaluation model was relatively single. Only 16 (4.7%) literatures tried to construct the training effect evaluation system of nurses, which was not based on some theory or hypothesis, which had not been effectively put into practice. Conclusion The effect evaluation system of nurse training needed to be done in depth, such as reconstruction of the new training evaluation model, the establishment of training evaluation system of different professional, different levels, different positions, to research evaluation methods, evaluation criteria and clinical practice testing, to design, training evaluation management software etc.

Objective To explore the experience in critical nursing intervention for patients with postpartum hemorrhoids. Methods 100 primiparas with hemorrhoids who had normal delivery in our hospital were assigned, by odd or even number of the delivery date, into the control group including 53 patients or the observation group including 47 patients. Patients in the control group received normative vital signs monitoring, hemorrhoid swelling observation, bowel movement observation, medication observation, dietary nursing, psychological support, treatment for complications, health education, and discharge instruction, etc. Patients in the observation group received critical analysis and nursing in addition to the care received in the control group. The hemorrhoid pain intensity and patient-perceived comfort were observed for both groups at 2 h, 24 h and 48 h postpartum. Results The hemorrhoid pain intensity score at 2 h postpartum was (1.30 ± 0.46) points and (1.32 ± 0.47) points , respectively, in the observation group and control group;the comfort score at 2h postpartum was (5.04 ± 0.83) points and (5.11 ± 0.82) points , respectively, in the observation group and control group, without statistically significant difference (t=0.245, 0.426, P>0.05);the hemorrhoid pain intensity score at 24h postpartum was (2.85 ± 0.62) points and (3.53 ± 0.58) points , respectively, in the observation group and control group;the comfort score at 24 h postpartum was (5.85 ± 1.02) points and (4.17 ± 0.89) points , respectively, in the observation group and control group, with statistically significant difference (t=5.643, 8.784, P < 0.01) ;the hemorrhoid pain intensity score at 48 h postpartum was (2.55 ±0.50) points and (3.42 ± 0.50) points , respectively, in the observation group and control group;the comfort score at 48h postpartum was (7.47 ± 1.10) points and (6.47 ± 0.89) points , respectively, in the observation group and control group, with statistically significant difference (t=8.606, 5.001, P<0.01). Conclusions Critical nursing intervention for postpartum patients with hemorrhoids could significantly alleviate hemorrhoid pain, improve patient comfort, and facilitate postpartum recovery.

Objective To integrate nurses' humanistic quality into professional nursing training, in order to improve humanistic quality and nursing quality. Methods Nurses with working years within 6 years in our hospital were chosen from May, 2009 to May, 2010. And through non-professional reading, specified materials reading, multimedia lectures, particularly the refining and essence of reading reports, humanities knowledge and humanistic care consciousness was melted into personal morality, words, deeds and cultivation. Investigation about satisfaction degree was carried out among patients before and after the training. Results Satisfaction degree of patients increased after training, so did the satisfaction degree of nurses to reading reports. Conclusions To integrate nurses' humanistic quality into professional nursing training can improve humanistic quality of nurses and nursing quality. Nurses' professional image will be increasingly standard, overall quality and service quality can be improved, and thought of dedicated service for patients will be consolidated.