[Objective]To establish an animal model of heterotopie ossification(HO)induced by Achilles' tenotomy in Sprague-Dawley(SD)rat and to investigate its possible mechanism.[Method]Thirty six male SD rats were divided into 3 groups(n=12).In sham group,incision and suturation were performed only in the left leg.Partial tenotomy was performed in the left Achilles tendons in PAT group.In AT group,tenotomy was performed in the left Achilles tendons to establish animal model of HO.X-ray and histological examinations were made at 6 and 10 weeks after operation.[Result](1)No HO occurred in the sham and PAT groups.(2)In AT group,3 rats showed cartilage and bone formation on X-rays while the remaining 3 rats showed chondrification in histological examination at 6 weeks after operation.At 10 weeks all rats showed bone formation with trabecular bone.This kind of HO developed through a process of endochondral ossification.[Conclusion]Tenotomy is a simple,effective and feasible method to induce HO.

Objective To compare the curative effects of multi-level puncture versus single-level puncture percutaneous vertebroplasty(PVP) for treatment of multiple-level osteoporotic vertebral body compression fractures(OVCF) in senile patients.Methods From June 2008 through November 2014,89 senile patients with fresh multiple-level OVCF underwent PVP guided by C-arm fluoroscopy in prone position.Of them,51 received PVP in which the vertebrae of multiple levels were simultaneously punctured for bone cement injection while the other 38 received PVP in which the vertebrae of multiple levels were punctured one by one for bone cement injection.The 2 groups were compatible with no significant differences in preoperative demographic data (P ＞ 0.05).The 2 groups were compared in terms of visual analgesic scale (VAS),operation time for a single vertebra,fluoroscopy times for a single vertebra,bone cement injection amount for a single vertebra,and extraosseous cement leakage.Results PVP procedures were successful in both groups without serious complications.The VAS scores in both groups at 2 days post-operation were significantly lower than those at pre-operation(P ＜ 0.05).The operation time and fluoroscopy times for a single vertebra in the multi-level puncture PVP group were significantly less than those in the single-level puncture PVP group (P ＜ 0.05).There were no significant differences between the 2 groups in bone cement injection amount for a single vertebra or extraosseous cement leakage (P ＞ 0.05).Conclusions The curative effects of multi-level puncture and single-level PVP are satisfactory for senile OVCF,but multi-level puncture PVP may lead to less operation time and less X-ray exposure.

Objective To investigate the effects of local injection of Botulinum toxin A (BTX-A) on hemifacial spasm guided by periph-eral nerve stimulation. Methods 57 patients with hemifacial spasm from January, 2012 to June, 2015 received local multi-point injection of BTX-A guided by peripheral nerve stimulation. The grades of facial spasm were evaluated before, 72 hours and 6 months after treatment. They were followed up for 3-9 months. Results The spasm reduced in the patients both 72 hours and 6 months after treatment (χ2=4.946, P<0.05). The incidence was 98.25% of satisfaction and 92.99% of very well 72 hours after treatment, while it was 91.23% and 78.95% 6 months after treatment. The relief of spasm was maintained for (23.1 ± 2.3) weeks. Conclusion Injection of BTX-A guided by peripheral nerve stimulation is effective on hemifacial spasm for a long time.

Three hundred patients with Graves′ disease (GD) were analysed according to clinical data, which showed a negative correlation between thyroid 131 I turnover and effective half life, suggesting that 131 I turnover is an important parameter for GD therapy of 131 I dose calculation, and a higher cure rate is found in GD patients whose 131 I turnover is less than 1.

0.05.Only 1 of 28 (3.6%) showed positive survivin signal in the control group.The difference of the positive rate of survivin between groups of TCCB and control was significant ( P

The characteristic feature, tissue structure and chemical composition of primitive bark and one to four years regenerated bark of Eucommia ulmoides were compared, and the contents of elastic rubber thread, chlorogenic acid and ethanolic extract of the two barks were determined Res ilts showed that regeaerated bark is of the same quality as primitive bark.

Objective To evaluate stepping DSA technology in lower extremity artey disease. Methods 12 cases performed with stepping DSA in lower extremity artery were examined with TOSHIBA Infinixi digital subtraction angiography system, of which, 8 ones were also examined with conventional DSA. Results Stepping DSA technology could decrease the dosage of contrast agent and the examination.

Objective To investigate the exercise self-regulatory efficacy and its influencing factors of chronic obstructive pulmonary disease(COPD) patients with acute exacerbation. Methods A total of 126 patients with acute exacerbation of COPD were selected by convenient sampling method and investigated with a demographic questionnaire, EXercise Self-Regulatory Efficacy Scale and Social Support Rating Scale. Results The mean score of exercise self-regulatory efficacy was (79.97 ± 19.78) points at medium level. Multiple-factor analysis showed that availability of social support, other diseases, age and dyssomnia were influencing factors of exercise self-regulatory efficacy of COPD patients with acute exacerbation(t=-3.082-5.351, P<0.01 or 0.05). Conclusions The level of exercise self-regulatory efficacy of COPD patients with acute exacerbation need to be improved, and we should pay more attention to the social support system and the control of other diseases.

20 IU/ml) as predictor was 40.0% and 75.4% respectively. Conclusions The incidence of malignant neoplasm among postmenopausal bleeding women aged 60 and over is 24.2%,ultrasonographic evaluation and CA125 value are reliable and important for diagnosis accurately. Large operation is safe and feasible in aged women.

Objective To investigate the changes and significance of early immune response in specific T cell with invasive pulmonary aspergillosis(IPA) patients. Methods Peripheral blood mononuclear cells (PBMCs) were separated from whole blood of 8 cases of healthy individuals (healthy group) and 24 cases of IPA patients (IPA group, including 6 cases of pathological diagnosis, 9 cases of clinical diagnosis and 9 cases of tentative diagnosis), and the heat-inactivated Aspergillus fumigatus spores (Conidia) was used as an antigen to stimulate PBMCs produce Aspergillus-specific T lymphocytes. Interferon-gamma (IFN-γ) secreation, type and ratio of cytokine synthesis was examined. Results In IPA group, dot enzyme linked immunosorbent assay(ELISPOT) showed that the positive rate of IFN-γin pathological diagnosis patients and clinical diagnosis patients (5/6,7/9) was higher than that in tentative diagnosis patients (3/9). The positive rate of IFN-γin IPA group was 62.5%(15/24), in healthy group was 0 (0/8), and there was significant difference (P<0.05). The levels of CD4+T and CD4+T/CD8+T in IPA group were 0.202 0±0.085 6 and 1.01±0.34, in healthy group were 0.3853±0.1265 and 1.55±0.41. The levels of CD4+T and CD4+T/CD8+T in IPA group were significantly lower than those in healthy group ( P<0.05 or<0.01). The level of CD 8+T in IPA group was 0.298 5±0.069 1, and in healthy group was 0.257 6±0.102 6. The level of CD8+T in IPA group was 05). Conclusion Conidia as antigen can induce the specific Th1-type immune response of IPA, and display the immune status of the IPA patients, and can provide new ideas and methods for the diagnosis and assessment of the disease.