1.Clinical application and evaluation of 4 kinds of siphilis serological detection method
Jing YIN ; Guihong XU ; Yan LIU
International Journal of Laboratory Medicine 2014;(23):3244-3245,3248
Objective To compare the value of four kinds of commonly used serological detection method for detecting Trepone-ma Pallidum,i.e.,Treponema Pallidum enzyme-linked immunosorbent assay(TP-ELISA),Treponema pallidum particle agglutina-tion test(TPPA),Treponema Pallidum rapid plasma reagin test(RPR)and Treponema Pallidum antibody detection(TP-AD,emul-sion method).Methods 5 870 specimens from outpatients and inpatients were screened by TP-ELISA.121 cases of detected posi-tive specimen were simultaneously detected by TP-AD,TPPA and RPR.Then the detection results were performed the comparative analysis.Results Among 5 870 specimens,121 cases were positive by ELISA,the detection rate was 2.06%.Among 121 positive cases,119 cases were positive by TPPA,the conformity degree was 98.34%,49 cases were positive by RPR,the conformity degree was 40.41%,113 cases were positive by TP-AD,the conformity degree was 93.38%.With the TPPA results as the standard,there was no statistically significant difference between TPPA and TP-AD(P >0.05),but there was statistically significant difference be-tween TPPA and RPR(P <0.01).Conclusion The four kinds of method have their applicability.ELISA d has good specificity and high sensitivity,and is simple to operate and suitable for the physical examination of large amount of pregnant women,parturients and normal people.TPPA has good specificity and high accuracy,is suitable for definite diagnosis.RPR is suitable for the monito-ring and the curative effect observation in the patients with the active stage of siphilis.Compared with ELISA,TP-AD has slightly less sensitivity,but good specificity and can be used for screening without specific instrument.
2.Investigation on post setting of mobile nurses in grade Ⅱ & Ⅲ hospitals
Yunyun WANG ; Zhenxian SHI ; Yan YANG ; Guihong YIN ; Feitong JIAO
Chinese Journal of Modern Nursing 2014;20(35):4504-4506
Objective To investigate the awareness , the nursing position demand and capacity requirements of mobile nurses , so as to provide the basis for setting and development of the mobile nurses . Methods Investigated 1 415 nursing staff from 120 hospitals in four provinces about mobile nurse awareness , willingness and ability requirements .Results The awareness of mobile nurses to nurses was 84.45%, only 20.83%of the hospital departments had mobile nurses .Position demand scores of tertiary hospitals , wards with bed utilization rate 90%or higher, and surgery departments respectively were (4.40 ±0.98), (4.46 ±0.87), and(4.35 ±1.04),more higher than that of second-class hospital, wards with bed utilization rate less than 90%, and medicine departments, and significant difference was statistically significant (t/F=20.17,207.49, 10.92,respectively;P<0.05).Conclusions The management and operation of mobile nurses in China is in the initial stage, mobile nurses are urgent needed in the surgical ward of tertiary hospitals .Mobile nurses should possess general nurses ability and emergency care skills .