Objective To evaluate the efficacy of hyperbaric oxygen in treatment of sudden deafness and to analyze related factors of deafness,in order to provide the basis for clinical treatment.Methods 72 cases with sudden deafness were admitted.41 cases (total of 43 ears) were given conventional treatment.31 cases (total of 32 ears) were given hyperbaric oxygen plus conventional treatment.The influencing factors of deafness in 32 cases were also analyzed.Results The total effective rate of hyperbaric oxygen therapy group was 81.25％,that of the conventional drug treatment group was 65.12％,the difference was statistically significant (P ＜ 0.05).In hyperbaric oxygen therapy group,the visiting time less than 24h,the effective rate was 100.00％,which was higher than visiting time 1 to 14 days (90.00％) and more than 15 days (37.50％) (P ＜ 0.05).The effective rate of visiting time between 10-20 days and more than 20 days was significant different (P ＜ 0.05).The effective rate from high to low was 15-34 years old,35-54 years old and ≥55 years old.The effective rate of 15-34 and 35-54 years old group was significantly higher than ≥55 group (P ＜ 0.05).The effective rate of mild deafness was 100.00 ％,which was significantly higher than moderate deafness 84.62％ and severe hearing loss 20.00％ (all P ＜ 0.05).Conclusion The effect of hyperbaric oxygen plus drug in treatment of sudden deafness was significant.The sudden deafness shoud be treated early in clinical by hyperbaric oxygen.

Objective To study the effects of different preservation methods on the isolated liver injury and regeneration.Methods Twelve Sprague-Dawley rats were randomly assigned to two groups:static cold storage (SCS) and hypothermic machine perfusion (HMP) (n =6 each).Histidinetryptophan-ketoglutarate solution (HTK) was used as preservation solution.The graft was preserved in HTK (4 C) for 6 h in SCS group,and that was perfused using HTK in HMP group.Liver tissue was obtained and fixed in 10％ neutral formalin for immunohistochemistry.The fresh liver tissue was collected and stored in-80℃ for RT-PCR and Western blotting analyses.Results Compared to SCS,HMP improved liver regeneration ability in terms of PCNA and ki67 detection and promoted cell proliferation in PCR levels (Cdc25A,cdk1,cdk6) and Western blotting levels (Cyelin D1,Cyclin E1).Conclusion HMP is superior to SCS in liver regeneration,and expected to be the ideal method for preservation of donor liver.