Objective; To study estrogen receptor gene Xbal and Pvu Ⅱ polymorphisms in patients with aggressive periodontitis (AgP). Methods; Xbal and Pvu Ⅱ DNA was extracted by Chelex-100 and amplified by PCR from buccal swabs of 48 cases of AgP patients and 60 normal controls. The PCR products were analyzed by polymerase chain reaction linked fragment length polymorphism (PCR-RFLP) assay. Results: There were significant differences of the distribution of Xba I genotype between AgP group and control group, female AgP group and female control group, male AgP group and male control group(P<0.05). There was no difference of Pvu D genotype distribution between patient group and control group (P>0.05). Multivariate Logistic Regression Analysis showed that male group was less susceptible to AgP than female group(OR =0. 352), the older was less susceptible to AgP than the younger(OR =0.950), and the xxXx genotype was less susceptible to AgP than XX genotype [OR(Xx) =0.224, OR(xx) = 0.678). Conclusion: Specific relationship is found between the susceptibility of AgP and the ER gene-Xbal polymorphism. People with XX genotype is more susceptive to AgP than xx, Xx genotypes.

Objective: To study the relationship between C-reactive protein (CRP) gene polymorphisms and the risk of chronic peri-odontitis and severe chronic periodontitis (CP) with type2 diabetes to confirm the effect of genetic factor in chronic periodontitis and chronic periodontitis with type2 diabetes. Methods; DNA was extracted by Chelex-100 from buccal swabs of patients who suffered from chronic periodontitis or chronic periodontitis with type2 diabetes and patients with healthy periodontium. PCR-RFLP was used to test the CRP genotype distribution. The correlationship between the incidence of chronic periodontitis and chronic periodontitis with type2 diabetes and CRP gene polymorphism was analyzed statistically. Results; There was no statistical difference in the distribution of CPR +1059 genotype and allele frequency between experiment group and control group (X~2 = 0. 223, P=0.994). The genotype and allele frequency distribution were in line with Hardy-Weinberg equilibrium. Conclusion; There is no correlation between CRP + 1059G/C single nucleotide polymorphisms and the susceptibility of chronic periodontitis as well as chronic periodontitis with type2 diabetes.

Objective To observe the influence of the short effect antihypertension drugs- nifedipine and medial effect antihypertension drugs- extended release nifedipine on the blood pressure variability (BPV) in essential hypertension(EH). Methods Twenty-five EH patients were underwent 24-hour noninvasive ambulatory blood pressure monitoring (ABPM) and observed their BPV respectively before taking drugs, after taking nifedipine and extended release nifedipine. Meantime,25 normotensive controls (NC) were observed. Results (1)BPV in EH group was higher than that in controlled group and the severer the rise of blood pressure, the more obvious the increase of BPV (P 0.05). Conclusions Nifedipine could increase BPV but extended release nifedipine did not change BPV while they decreased blood pressure. Effect of extended release nifedipine was better than nifedipine in decreasing blood pressure.

OBJECTIVETo study the relationship between endothelial nitric oxide synthase (eNOS) Glu298Asp gene polymorphism and chronic periodontitis (CP) with type 2 diabetes mellitus (T2DM).

METHODSDNA from patients' buccal swabs of CP, CP with T2DM, T2DM and health was isolated and extracted. The eNOS Glu298Asp gene polymorphism were assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) respectively.

RESULTSThe distribution of eNOS Glu298Asp genotype in CP group, T2DM group, CP with T2DM group and health group showed statistical differences (chi2 = 18.503, P = 0.005), and the gene frequency showed statistical differences (chi2 = 8.243, P = 0.041). Compared health group to CP with T2DM group, OR value of the genetype T was 0.962, 95% confidence interval lay in between 0.737 to 1.256, which showed T maybe a protective factor. While OR value of the genetype G was 1.043, 95% confidence interval lay in between 0.781 to 1.391, which showed G maybe a risk factor. However, neither T nor G was statistically significant.

CONCLUSIONBased on these findings, there are some association between eNOS Glu298Asp polymorphism and the risk of CP group, T2DM group, CP with T2DM group.

Chronic Periodontitis ; Diabetes Mellitus, Type 2 ; Gene Frequency ; Genotype ; Humans ; Male ; Middle Aged ; Nitric Oxide Synthase Type III ; Polymerase Chain Reaction ; Polymorphism, Genetic ; Risk Factors

Objective:To observe the effects of the periodontal subgingival scaling in different stages of standardized residency training of stomatology general medicine.Methods:Thirty residents in Affiliated Stomatology Hospital of Zhejiang University School of Medicine were recruited by stratified randomization and divided into three groups, including 1-month group, 3-month group and 6-month group. The residual ratio of clinical calculus was compared after periodontal subgingival scaling among groups. SPSS 23.0 was conducted for Kruskal-Wallis H test. Results:The mean residual ratio of clinical calculus in 3-month group and 6-month group were significantly lower than that in the 1-month group. In the 3-month group, the scaling could only achieve the effective results at the shallow pockets and anterior tooth area. Compared with the 1-month group, the 6-month group significantly decreased the residual ratio of clinical calculus for the sites with pocket depth of 4-6 mm, but there were still insufficiency to deal with the posterior tooth area.Conclusion:The 3-6 months residency training of periodontology could improve the effectiveness of the periodontal subgingival scaling. We should establish reasonable assessment and evaluation system according to different stages of residents, and the subgingival scaling training in the posterior areas and the distal interproximal sites should be reinforced in order to more effectively improve the quality of the standardized residency training.