Objective To develop a new-type animal dental stent for the experiment of canine pulp regeneration.Methods The stent was composed of an upper support frame,an oral limit bracket and a lower support frame,which were aligned from top to bottom.A sleeve joint was made for the connection structure of the bracket,and the height and the width both could be adjusted to adapt to different animals' mouths.The rubber dam could be spread into tension and concave form by rotating the upper and lower support frames' ends.Results By resizing the animal dental stent based on the structure of animal facies maxillaris,medical staffs could proceed the examination and treatment to animals effectively,including the throat examination,oral examination,oral care,dental treatment (such as root canal therapy and dental filling) and other medical behaviors.Conclusion The experimental result shows the feasibility of utilizing the stent in the process of animal experiment or diagnosis and treatment.Moreover,the stent proves the capacity in protecting the soft tissue,widening the vision of operation field,getting rid of the saliva contamination,which is worthy popularizing in animal experiments and animal medical care.

Objective:To investigate the improvement effect of acupuncture combined with early rehabilitation training on clinical efficacy, traditional Chinese medicine syndrome score, and neurological and motor functions of patients with acute cerebral infarction.Methods:A total of 76 patients with acute cerebral infarction who received treatment in the Department of Neurology at Jinhua Municipal Central Hospital from June 2019 to June 2022 were included in this prospective study. These patients were randomly divided into an observation group and a control group using the random number table method, with 38 patients in each group. The control group received early rehabilitation training, while the observation group received acupuncture combined with early rehabilitation training. The clinical efficacy was compared between the two groups. Pre- and post-treatment traditional Chinese medicine symptom scores and neurological and motor functions were compared between the two groups.Results:The overall response rate in the observation group was 89.47% (34/38), which was significantly higher than 67.59% (25/38) in the control group ( Z = 2.41, P = 0.016). After treatment, the scores of hemiplegia, mouth and eye deviation, and speech difficulty in both groups were significantly decreased compared with before treatment, and the observation group showed a greater degree of decline in these indices than the control group ( t = 4.73, P < 0.001; t = -10.58, P < 0.001; t = 6.42, P < 0.001). After treatment, the neurological deficit scale scores in each group were decreased compared with before treatment, and the observation group showed a greater degree of decline compared with the control group ( t = -7.33, P < 0.001). After treatment, the Fug-Meyer motor function scale and Barthel index scores in each group were significantly increased compared with before treatment, and the observation group showed a greater degree of increases compared with the control group ( t = 3.72, P < 0.001; t = -5.02, P < 0.001). After treatment, the bare hand muscle strength scores of the core muscles in both groups of patients were increased compared with before treatment, and the observation group showed a greater improvement compared with the control group ( t = 3.31, P < 0.001). Conclusion:Acupuncture combined with rehabilitation training can enhance clinical efficacy, improve traditional Chinese medicine syndrome scores, enhance neurological and motor functions, and strengthen core muscle strength in patients with acute cerebral infarction. This treatment approach is worthy of being further promoted in clinical practice.