one hundred and five Wistar rats were randomized into 3 groups and heterogenous decalcified bone matrix (HDBM) + CaCl_2+Na_2HPO_4+KH_2PO_4, HDBM + CaSO_4+Na_2HPO_4+KH_2PO_4, and HDBM + NaCl were implanted into the quadriceps muscle of both thighs of the animals respectively. They were monitored with ~3H-TdR and ~(45)Ca tracing to evaluate the efficacy of the 3 delivery systems to enhance osteogenesis. It was found that coadministration of CaSo_4, Na_2HPO_4, and KH_2PO_4 could significantly enhance the osteogenesis induced by HDBM. It is concluded that HDBM and the coadministration of Na_2HPO_4, KH_2-PO_4, and CaS0-4/CaCl_2 together will be much more effective to induce osteogenesis than HDBM is used singly.

OBJECTIVE: To investigate the application value of narrow band imaging (NBI) in early diagnosis of pharyngolaryngeal tumors. METHOD: A total of 106 patients received NBI endoscopy in the endoscopic diagnosis. Lesions found under the white-light endoscopy mode and NBI endoscopy mode were compared in the morphology of capillaries on lesiorn surface and the clarity of lesion rim. Biopsy was performed in suspected areas with those two endoscopies for the lesions found under white-light endoscopy and NBI endoscopy, the morphology of capillaries on the surface of lesion and the clarity of lesion boundary were compared between both. Biopsy was performed for suspected areas under two modes, and specimens were preserved in 10% formaldehyde for pathological examination. The characteristic, position and endoscopic diagnosis under two modes were recorded. All the patients underwent corresponding laryngeal tumor resection according to the histopathological result of biopsy, and the histopathological result of resected tissues was taken as the gold standard for diagnosis. The biopsy detection rate and biopsy correct detection rate of malignant lesions in two groups were calculated and statistical compared. RESULT: The diagnostic accuracy under white-light mode was 75.47%, while that under NBI mode was 96.23%, and the difference between them was statistically significant (χ² = 18.375, P < 0.01). The biopsy correct detection rate under white-light mode was 82.08%, while that under NBI mode was 95.28%, and the difference between them was statistically significant (χ² = 12.071, P < 0.01). The correct detection rate of malignant tumor under white-light mode was 48.15%, while that under NBI mode was 92.59%, and the difference between them was statistically significant (χ² = 10.083, P < 0.01). CONCLUSION Using NBI endoscopy to observe the morphological changes of capillaries on the pharyngolaryneal mucosa surface can increase the detection rate of early pharyngolaryngeal tumors, so it is worth to be widely applied.
Biopsy ; Early Detection of Cancer ; Endoscopy ; Humans ; Laryngeal Neoplasms ; diagnosis ; surgery ; Larynx ; surgery ; Narrow Band Imaging ; Pharyngeal Neoplasms ; diagnosis ; surgery