1.Experimental study of anti-metastasis effect of urokinase amino-terminal fragment gene on human breast cancer cells
Fuxiang ZHU ; Guichun XING ; Fuchu HE
Chinese Journal of Oncology 2001;23(2):115-117
Objective To explore the suppressive effects of urokinase amino-terminal fragment (ATF) gene on metastatic potential of human breast cancer cell line MCF-7. Methods A pcDNA3-ATF plasmid containing ATF cDNA under CMV promotor/enhancer control was constructed and transfected into MCF-7 cells by lipofectin. The expression of of uPA/uPAR and ATF in MCF-7 cells were analyzed by RT-PCR and Western blot. The effect of ATF expression on invasiveness in vitro, tumorigenesis and metastasis in vivo of MCF-7 cell was investigated. Results MCF-7 cells displayed an overexpression of uPA/uPAR. Expression of ATF was detected after ATF gene-transfection. The invasive capacity of ATF gene-transfected MCF-7 cells was decreased significantly. Although the tumorigenesis was not affected, the in vivo metastasis of ATF gene-transfected MCF-7 cells was remarkably inhibited. Conclusion Suppression of invasiveness and metastasis of ATF-transfected MCF-7 cells is perhaps due to a competitive inhibition of interaction with endogenous uPA/uPAR.
2.Experimental study of anti-metastasis effect of urokinase amino-terminal fragment gene on human breast cancer cells
Fuxiang ZHU ; Guichun XING ; Fuchu HE
Chinese Journal of Oncology 2001;23(2):115-117
Objective To explore the suppressive effects of urokinase amino-terminal fragment (ATF) gene on metastatic potential of human breast cancer cell line MCF-7. Methods A pcDNA3-ATF plasmid containing ATF cDNA under CMV promotor/enhancer control was constructed and transfected into MCF-7 cells by lipofectin. The expression of of uPA/uPAR and ATF in MCF-7 cells were analyzed by RT-PCR and Western blot. The effect of ATF expression on invasiveness in vitro, tumorigenesis and metastasis in vivo of MCF-7 cell was investigated. Results MCF-7 cells displayed an overexpression of uPA/uPAR. Expression of ATF was detected after ATF gene-transfection. The invasive capacity of ATF gene-transfected MCF-7 cells was decreased significantly. Although the tumorigenesis was not affected, the in vivo metastasis of ATF gene-transfected MCF-7 cells was remarkably inhibited. Conclusion Suppression of invasiveness and metastasis of ATF-transfected MCF-7 cells is perhaps due to a competitive inhibition of interaction with endogenous uPA/uPAR.
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