1.Effects and related mechanisms of preconditioning with puerarin on hippocampus CA1 region neuronal injury after focal cerebral ischemia-reperfusion
Acta Universitatis Medicinalis Anhui 2015;(6):723-726
Objective To observe the effect of puerarin preconditioning on hippocampal CA1 region neuronal injury and further explore its mechanisms. Methods 30 male Sprague-Dawley rats were randomly assigned into sham, model,and puerarin preconditioning ischemia-reperfusion groups. The middle cerebral artery was occluded for 60 min by an intraluminal filament before reperfusion to induce transient focal cerebral ischemia reperfusion rats. Puer-arin preconditioning groups rats received puerarin(100 mg / kg,i. p)1 h before ischemia. The histological changes of hippocampal CA1 region neurons were measured by HE staining on the 5th day after reperfusion. The expressions of Caspase-3 and Caspase-9 proteins in the CA1 region were examined by immunohistochemistry staining. Results The results of HE staining showed that,compared with the model group,puerarin preconditioning significantly re-duced the number of cerebral ischemia induced injury of hippocampal CA1 region neurons(P < 0. 05). Immunohis-tochemistry data showed that the expressions of Caspase-3 and Caspase-9 in the hippocampal CA1 region of puerarin preconditioning group were significantly lower than that of model group(P < 0. 05). Conclusion The results indi-cate that puerarin preconditioning 1 h before ischemia exerts neuroprotective effects on focal cerebral ischemia-reperfusion induced neuronal injury. The neuroprotective mechanisms of puerarin may attribute to inhibiting the ex-pressions of Caspase-3 and Caspase-9 protein.
2.Protective Effect of Active Principle of Glycyrrhiza Uralensis Fish(Lx)on Anaphylaxis in Mice
Gui-Zhen YANG ; Gui-Lan WANG ; Li WANG
Chinese Journal of Immunology 1985;0(01):-
The systemic anaphylaxis murine model was induced by the injection of oval albumin (OVA)in aluminum hydroxide gel adjuvant. The death rate varied with the mouse species: 40-90% in Swiss and 69% in average,no death in C57BL/6 and 615 but some symptoms. developed. Swiss mice was adopted in this experiment.90% of mice pretreated with Lx remained living after allergen challenge,but only 20% in normal control. For elucidation the possible mechanism of Lx action,the study was carried out on detection of anti-OVA IgE specific antibody and histamin concentration in lung tissues. Passive cutaneous anaphylaxis(PCA)titers for anti-OVA IgE antibody in serum ranged from undilution to 160 in control group and not detectable in group pretreated with Lx. In addition, after absorption of PCA positive sera with anti-IgG antibody.the PCA activity was not affected. Histamin content was 1.54?0.24?g/ml in Lx treated mice caopared to 2.69?0.67?g/ml in control group(p
3.The Effect of Ginsenoside(GS) on NKC-IFN-IL-2 Regulatory Network and It's Tumor Inhibiting Effect
Chinese Journal of Immunology 1985;0(01):-
The main results of the article are as follows:GS(10ug/ml,25ug/ml,50ug/ml)could augment NK activity of murine spleen cells in vitro(p
4.MYO minisatellite DNA probe and sub—cloning of MYO fragment
Chinese Journal of Immunology 1985;0(01):-
The initial study of the tumour DNA fingerprints using MYO minisatellite DNA probe wascarried out,and then,by means of DNA recombinant techniques,the fragment of MYO min-isatellite DNA probe obtained from plasmid pUC19-MYO was inserted into plasmid pGEM-4Z containing RNA polymerase promotor,thus a sub-clone refferred as pGEM-4Z-MYOwas constucted.That made an offer of the conditions of preparing RNA probe in order to in-cerase the sensitivities of DNA fingerprinting and laid a foundation for raised the efficiency of de-tecting the polymorphism of the minisatellite DNA.
5.Transport of PLGA nanoparticles across Caco-2/HT29-MTX co-cultured cells.
Zhen WEN ; Gang LI ; Dong-Hai LIN ; Jun-Teng WANG ; Li-Fang QIN ; Gui-Ping GUO
Acta Pharmaceutica Sinica 2013;48(12):1829-1835
The present study is to establish Caco-2/HT29-MTX co-cultured cells and investigate the transport capability of PLGA nanoparticles with different surface chemical properties across Caco-2/HT29-MTX co-cultured cells. PLGA-NPs, mPEG-PLGA-NPs and chitosan coated PLGA-NPs were prepared by nanoprecipitation method using poly(lactic-co-glycolic acid) as carrier material with surface modified by methoxy poly(ethylene glycol) and chitosan. The particle size and zeta potential of nanoparticles were measured by dynamic light scattering. Coumarin 6 was used as a fluorescent marker in the transport of nanoparticles investigated by confocal laser scanning microscopy. The transport of furanodiene (FDE) loaded nanoparticles was quantitively determined by high performance liquid chromatography. Colchicine and nocodazole were used in the transport study to explore the involved endocytosis mechanisms of nanoparticles. Distribution of the tight junction proteins ZO-1 was also analyzed by immunofluorescence staining. The results showed that the nanoparticles dispersed uniformly. The zeta potential of PLGA-NPs was negative, the mPEG-PLGA-NPs was close to neutral and the CS-PLGA-NPs was positive. The entrapment efficiency of FDE in all nanoparticles was higher than 75%. The transport capability of mPEG-PLGA-NPs across Caco-2/HT29-MTX co-cultured cells was higher than that of PLGA-NPs and CS-PLGA-NPs. Colchicine and nocodazole could significantly decrease the transport amount of nanoparticles. mPEG-PLGA-NPs could obviously reduce the distribution of ZO-1 protein than PLGA-NPs and CS-PLGA-NPs. The transport mechanism of PLGA-NPs and mPEG-PLGA-NPs were indicated to be a combination of endocytosis and paracellular way, while CS-PLGA-NPs mainly relied on the endocytosis way. PEG coating could shield the surface charge and enhance the hydrophilicity of PLGA nanoparticles, which leads mPEG-PLGA-NPs to possess higher anti-adhesion activity. As a result, mPEG-PLGA-NPs could penetrate the mucus layer rapidly and transport across Caco-2/HT29-MTX co-cultured cells.
Biological Transport
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Caco-2 Cells
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Chitosan
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chemistry
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Coated Materials, Biocompatible
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chemistry
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Coculture Techniques
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Drug Carriers
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Furans
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administration & dosage
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chemistry
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metabolism
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HT29 Cells
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Heterocyclic Compounds, 2-Ring
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administration & dosage
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chemistry
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metabolism
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Humans
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Lactic Acid
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chemistry
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Nanoparticles
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Particle Size
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Polyethylene Glycols
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chemistry
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Polyglycolic Acid
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chemistry
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Zonula Occludens-1 Protein
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metabolism
6.Study of effects of complex aerobatics on serum insulin, cortisol and angiotensin II.
Ming Gao LI ; She Zhen QIN ; Gui Xi MA ; Lang En XU ; Xin Hua ZHANG
Korean Journal of Aerospace and Environmental Medicine 1993;3(1):112-118
No abstract available.
Angiotensin II*
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Angiotensins*
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Hydrocortisone*
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Insulin*
7.Influence of complex aerobatics on serum gastrin level of pilots.
Gui Xi MA ; She Zhen QIN ; Ming Gao LI ; Lan Gen XU ; Xin Hua CHANG
Korean Journal of Aerospace and Environmental Medicine 1993;3(2):71-72
No abstract available.
Gastrins*
8.Expression of plasma miR-199a-5p and miR-200c-3p and its clinical relevance in gastric carcinoma
Jinli TANG ; Feng YAN ; Xiaoming WANG ; Mengyuan GE ; Zhen GUI ; Jinchang LI ; Mingchen ZHU
Chinese Journal of Laboratory Medicine 2015;(6):402-406
Objective To explore the relative expression of plasma miR-199a-5p and miR-200c-3p in gastric adenocarcinoma cancer(GAC) patients and its clinical value.Methods Case-control study was used in this research.The relative expression of plasma miR-199a-5p and miR-200c-3p from 47 GAC patients and 50 healthy controls were determined by RT-PCR ( TaqMan Probe method).Meanwhile, the association with age, gender, tumor location, size, degree of differentiation, TNM stage, lymph node metastasis and other clinical pathological parameters were analyzed.The expression of these two miRNAs in plasma of 30 GAC patients during preoperation was compared with their expression 6-8 days after radical surgery.The sensitivity and specificity of plasma miRNAs expression for the diagnosis of GAC were analyzed using the receiver operating characteristic ( ROC ) curve.SPSS20.0 statistical software was used for statistical analysis.T-test, paired t-test and one-factor ANOVA were used for normal distribution of quantitative data.Results The plasma level of miR-199a-5p in GAC patients was significantly lower(1.05 ±0.22) (t =3.058,P =0.003), while miR-200c-3p was significantly higher(15.15 ±3.02) (t =-2.854,P=0.006), when they were compared with those in controls(26.80 ±8.38, 3.39 ±0.87).Low miR-199a-5p expression in GAC patients were associated with lymph node metastasis ( F =4.725, P =0.029) and the differentiation degree of gastric cancer(F=3.854,P=0.032).The relative expression of miR-199a-5p in postoperative plasma was significantly increased(t=-3.814,P=0.001), but the relative expression of miR-200c-3p was significantly reduced when compared to the preoperative samples(t=2.978, P=0.006).Area under the ROC curve of miR-199a-5p, miR-200c-3p and combined miR-199a-5p and miR-200c-3p were 0.692, 0.792 and 0.798, the sensitivity and specificity were 87%,97%,92.5% and 43%,54%, 65%, respectively.Conclusion Combined detection of miR-199a-5p and miR-200c-3p in plasma has a higher sensitivity and specificity than the conventional tumor marker CEA and CA19-9, and may be a useful combination for gastric cancer diagnosis.
9.Combined effect of heat and noise on plasma angiotension II and adreomedullin content in pilots.
Yu LIU ; Gui-xi MA ; Shi-zhen QIN ; Wen-bin LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(2):134-135
Adult
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Aircraft
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Angiotensinogen
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analogs & derivatives
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blood
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Hot Temperature
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adverse effects
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Humans
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Male
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Neuropeptides
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blood
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Noise
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adverse effects
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Occupational Exposure
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adverse effects
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Stress, Psychological
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blood
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etiology
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Time Factors
10.Mechanism of hepatitis B virus infection of trophoblast cells and hepatitis B virus intrauterine infection
Han BAI ; Yang DING ; Lin ZHANG ; Li MA ; Gui-Zhen ZHAO ; Xiao-Guang DOU ;
Chinese Journal of Infectious Diseases 2007;0(11):-
1.0?10~7 copies/mL. The HBsAg IHC staining positive cells could be observed in 6 placental tissues and 3 fetus' liver tissues,and HBcAg was also positive in 1 case of fetus' liver tissue.After co-incubating the tropho- blastic cells and HBV DNA positive serum in vitro,HBsAg expression and HBV DNA could be detected.Apoptosis of HBV-infected trophoblastic cells increased,which was demonstrated by in vivo and in vitro experiments and the apoptosis of placental cells was correlated with the cord blood HBV DNA level.The results of in vitro experiments showed that the apoptosis of trophoblastic cells increased with the elongation of infection time.After 6 months,1 of 12 newborns was positive for HBsAg,HBeAg and anti-HBc,6 was positive for anti-HBs.Conclusions The mechanism of HBV intra-uterine infection may be that HBV breaches the placental barrier and infects the fetus.The localization and replication of HBV in fetal tissues and organs are probably the important factors of chronic HBV infections in neonates.The apoptosis of trophoblastic cells may be the protective mecha- nism for the placental barrier to block the HBV intra-uterine transmission.