To understand the adverse drug reaction (ADR) induced by Mailuoning injection. 162 ADRs due to the drug were retrieved from national medical journals of 1988-2007 for statistics. It was shown that there was no relationship between ADR and dosage, but ADR appeared mostly in middle-aged and old groups, and more in male than in female. The occurrence of ADR was commonly within 30 min after injection. It involved injuries of various systems and organs. As for the 123 cases of allergy, 38 were anaphylactic shock (accounting for 23.46%), two people died. ADR characterized by immediate type on initial use and tachy type. It should be paid more attention to the Mailuoning injection for the immediate hypersensitivity reaction (such as anaphylactic shock).
Adolescent ; Adult ; Age Distribution ; Aged ; Aged, 80 and over ; Anaphylaxis ; chemically induced ; epidemiology ; pathology ; Child ; Dose-Response Relationship, Drug ; Drug-Related Side Effects and Adverse Reactions ; epidemiology ; mortality ; Drugs, Chinese Herbal ; administration & dosage ; adverse effects ; Female ; Humans ; Injections ; Male ; Middle Aged ; Sex Distribution ; Time Factors

OBJECTIVETo explore the association of the glycophorin A(GPA) gene mutation in peripheral erythrocytes and chronic benzene poisoning.

METHODSSixty-three patients with chronic benzene poisonings and 45 benzene-exposed workers who were engaged in the same job title were investigated. Fluorescence immunolabeling technique and flow cytometry were used to detect GPA mutation frequency in peripheral read blood cell.

RESULTSA significant decrease in WBC count and neutrophil count was found in patients with chronic benzene poisoning compared with control individuals (P<0.01). The WBC count and neutrophil count both decreased along with the GPA-NN frequency, and the trends were significant(P<0.05).Both WBC counts and neutrophil counts decreased as the frequency, and trends were significant(P<0.05). GPA-NN frequency increased along with the accumulative exposure score, and the trend was significant (P = 0.0026). There was no significant trend between the GPA-Nphi frequency and the accumulative exposure score (P = 0.2037).

CONCLUSIONA decrease in WBC count and neutrophil count is found in patients with chronic benzene poisoning, which can arise from genetic damage in bone marrow stem cells, namely gene-duplicating mutations (NN) at the GPA locus in bone marrow cells of MN-heterozygous subjects, GPA-NN mutagens contributed to the pathogenesis of chronic benzene poisoning.

Adult ; Benzene ; poisoning ; Bone Marrow Cells ; pathology ; Case-Control Studies ; Erythrocytes ; pathology ; Female ; Genetic Variation ; Glycophorin ; genetics ; Humans ; Leukocyte Count ; Male ; Middle Aged ; Mutation Rate ; Neutrophils ; pathology

OBJECTIVETo prepare the cross-linked chitosan/poly(acrylic acid)/poly (ethylene oxide) nanofibrous membrance loaded with pseudo-ginseng and to determine its characteristics.

METHODSPseudo-ginseng entrapped in chitosan, poly (acrylic acid), poly (ethylene oxide) nanofibrous membrane loaded with pseudo-ginseng was prepared by electrospinning and thermal treatment method. The surface morphology of fiber membrane was observed by scanning electron microscopy and the chemical structures were characterized by infrared spectroscopy; the thermal decomposition temperature was analyzed by the thermogravimetric analysis. UV-Vis spectra were used to evaluate the in vitro release properties.

RESULTSThe average diameter of the prepared nanofibrous particles was (181 ± 71) nm. The tensile strength of fiber membrane increased by 35.3% and the decomposition temperature increased from 197℃ to 208℃ after crosslinking. Compared with casting film, the structure of fiber membrane increased the release rate and the overall amount of active components from pseudo-ginseng.

CONCLUSIONThe preparation of chitosan fiber loaded with pseudo-ginseng is simple and the dispersion of pseudo-ginseng is homogeneous. This fibrous pseudo-ginseng exhibited good release performance, providing a new Chinese medicine formulation.

Acrylic Resins ; chemistry ; Chitosan ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Microscopy, Electron, Scanning ; Panax ; chemistry ; Polyethylene Glycols ; chemistry ; Temperature

Objective To discuss the influence of pomegranate polyphenols in cognitive impairment of chronic cerebral hypo-perfused rats.Methods Healthy male Wistar rats were selected in our study and randomly divided into sham-operated group,bilateral common carotid artery occlusion/two-vessel occlusion group (2VO control group) and 2VO+PJ (pomegranate Juice) group.The chronic cerebral hypo-perfused rat models were established by permanent occlusion of bilateral common carotid arteries of rats; one week after surgery,the rats in the first two groups were fed with sugar water and those in the last group were fed with pomegranate juice at a dosage of 2 mL/d by gavage for 7 weeks.Then,spatial learning and memory abilities were assessed using Morris water maze test.After that,the levels of superoxide dismutase (SOD) and malondialdehyde (MDA) in the cortex and hippocampus were assessed by biochemical determinations.The protein expression level of 4-hydroxynonenal (4-HNE) was detected by Western blotting.Results The time of escape latencies in 2VO control group was longer while the time spent in object square was shorter than those of sham-operated group (P＜0.05); the time spent in the object square of 2VO+PJ group ([6.18±1.32] s) was significantly longer as compared with that of 2VO control group ([3.96±1.65] s,P＜0.05),and that of the escape latencies was shorter as compared with that of 2VO control group (P＜0.05).The SOD levels in the 2VO+PJ group (the cortex:[19.79±3.34] U/mg prot,the hippocampus:[20.87±0.77] U/mg prot) were significantly higher than those of 2VO control group (the cortex [15.79±0.39],the hippocampus [18.90±0.81],P＜0.05).The MDA levels in the 2VO+PJ group (the cortex:[1.21±0.29] nmol/mg prot,the hippocampus [2.31±0.35] nmol/mg prot)were obviously lower than those of 2VO control group (the cortex [1.74±0.42],the hippocampus [2.86±0.51],P＜0.05).The 4-HNE level in the 2VO+PJ was significantly lower than that of 2VO control group (P＜0.05).Conclusion Pomegranate polyphenol can improve the cognitive impairment in rats after chronic cerebral hypoperfusion,which may be related to its anti-oxidative effect.

Study on features of acupoints with resistance test in the past half century is reviewed in this article. Mechanism and technology of the method are introduced as well as its shortcomings. The determination method of signal transmission along meridians with the combination of electrical network theories and practice is advanced. And the result of a series experiments on one meridian at the superficial part of the body are given as well. Thus, it is concluded that the signals of the point-in/point-out and the signals along a non-meridian path with the same distance are significantly different, which gives a verification of the feasibility of the method by using electrical network theories to set out characteristics of signal transmission along meridians dynamically.
Acupuncture Points ; Electrophysiological Phenomena ; Electrophysiology ; instrumentation ; methods ; Humans ; Meridians ; Signal Transduction

Objective To identify the difference in the central venous pressure measured via two different approaches. Methods We had searched the databases including PubMed, Cochrane Library, Embase, China National Knowledge Infrastructure ( CNKI) , VIP Database and Wanfang Database, and Google Scholar was used for supplement search. All the experimental studies with self-control design that investigated the difference in the central venous pressure measured via two different approaches from the database foundation of until 2014 were included. The literature exclusion and data collection were performed independently by two investigators. The literature quality was evaluated by the 2nd edition of quality assessment of diagnostic accuracy studies tool (QUADAS-2). All the studies enrolled were assigned to high-quality and low-quality groups for subsequent stratified meta-analysis. Results A total of 8 studies involving 273 samples were entered in the final analysis. Pooled analyses from all studies revealed a statistically significant difference between the two approaches ( P=0. 002), and the pooled deviation is 0. 436 (95% CI:0. 166-0. 707). Conclusions Consideration of minor difference between the two approaches showed by meta-analysis, peripherally-inserted central catheter ( PICC) remains feasible to measure central venous pressure. The consistency of the two approaches used for measuring central venous pressure requires further validation by large-scale clinical trials.

OBJECTIVETo investigate the clinical manifestations and risk factors of the patients from developmental dysplasia of the hip(DDH) family.

METHODSDetailed epidemiology investigation, physical examination, functional movement assessment, lab test and X-ray examination were applied to the whole members of a DDH family.

RESULTSIn the family with 9 generations and 218 persons, the incidence of DDH was 31.03% in 145 survivors. Patients mainly manifested bilateral knee and hip joint pain, flexion contracture of hip, limitation in internal and external rotation of hip; a few had arthritic functional disorder, deformation, and limp. The radiography illustrated shallow acetabulum with increased inclination, which encompassed the femoral head badly. Deformation of the femoral head, narrow joint space and osteophyte were also found by X-ray examination. The main risk factors of DDH were genetic factors, gender, birth season etc. The son or daughter with one or two DDH parents had a higher risk for developing DDH than those with no DDH parents. Furthermore, first-degree relatives of the DDH patients also had a greater chance to develop DDH than second-degree relatives and third-degree relatives. The incidence among females was higher than males, and the family member who was given birth in winter had a highest risk for developing DDH. However, there was no difference between incidence of DDH in children and youths and in adults; the incidence of DDH in the immigrants with no blood relationship also did not differ from the incidence of DDH in the family member.

CONCLUSIONThe genetic factors play an important role in the development of DDH, so do the environmental factors.

Adolescent ; Adult ; Age Factors ; Aged ; Aged, 80 and over ; Child ; Family Health ; Female ; Hip Dislocation, Congenital ; diagnosis ; genetics ; Humans ; Male ; Middle Aged ; Pedigree ; Risk Factors ; Sex Factors ; Young Adult

Chromosome 13q14 deletion is one of the most common cytogenetic abnormalities in multiple myeloma (MM). LSI (locus-specific identification)-RB1 (13q14.1-14.2 region) and LSI-D13S319 (13q14.3 region) probes are usually used to detect 13q14 deletion. The aims of this study was to compare the incidence of chromosome 13q14.1-14.2 and 13q14.3 deletion and to detect 13q14 deletion size and number of involved cells in MM patients. The chromosome 13q14 region was detected by fluorescence in situ hybridization using probes LSI-RB1 and LSI-D13S319 in plasma cells of 112 MM patients. The results showed that 47.3% (53 out of 112) MM patients had both LSI-RB1 and LSI-D13S319 13q14 deletion (cut-off value: 7%), and the deletion rates detected by probes LSI-RB1 and LSI-D13S319 were accordant. The positive rates of 13q14 deletion were 46.4% and 47.3% respectively when the cut-off level was increased to 20%, and the corresponding rate was 98%. MM patients carrying 13q14 deletion showed 18% - 98% (median value: 72.5%) and 22% - 98.5% (median value: 76.5%) of deleted nuclei involving the RB1 and the D13S319 locus (P = 0.38). There were 67.9% (36 out of 53) and 66% (35 out of 53) cases carrying > 65% of 13q14.1-14.2 and 13q14.3 deleted nuclei as high proportion deletion patients, respectively (P = 0.188). The positive rate of the high proportion deletion patients had still no difference between LSI-RB1 and LSI-D13S319 groups when the cut-off value was defined as 85% (P = 0.439). In conclusion, in this cohort of 112 MM patients, there was no significant difference between the LSI-RB1and LSI-D13S319 probes to detect 13q14 deletion. Both LSI-RB1 and LSI-D13S319 probes can be selected to detect 13q14 deletion in MM patients. All the 53 MM patients with 13q14 deletion had deletions of 13q14.1-14.2 and 13q14.3 regions, which is a large deletion as one of the important characters in MM patients with 13q14 deletion.
Chromosome Deletion ; Chromosomes, Human, Pair 13 ; Female ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Male ; Middle Aged ; Multiple Myeloma ; genetics

Angioimmunoblastic T-cell lymphoma (AITL) is a rare, distinct subtype of peripheral T-cell lymphoma, possessing an aggressive course and poor prognosis with no standard therapy. Twelve patients who have failed at least two initial CHOP or CHOP-like regimens were enrolled in this study and treated with individualized cyclosporine (CsA), prednisone (PDN), and monthly, high-dose intravenous immunoglobulin (HDIVIG). The dose of CsA was adjusted individually based on the blood trough concentration of CsA and renal function. All patients were examined for response, toxicity and survival. The most significant toxicities (≥ grade 2) were infection (16.7%), renal insufficiency (8.3%), hypertension (8.3%), diabetes (8.3%) and insomnia (16.7%). Discontinuation of treatment occurred in one patient (8.3%) due to grade 3 renal toxicity and subsequent grade 4 pulmonary infection. Treatment-related death was not observed. The overall response rate was 75.0% (complete response, 33.3%; partial response, 41.7%). With a median follow-up of 25.5 months, the median duration of response was 20 months (range, 12 to 49 months) and the median progression-free survival (PFS) was 25.5 months (range, 10 to 56 months). The 2-year PFS rate was 81.5%. Our findings indicate the combination of CsA, PDN and HDIVIG is an effective salvage regimen for refractory or relapsed AITL with predictable and manageable toxicity.
Aged ; Antineoplastic Combined Chemotherapy Protocols ; therapeutic use ; Combined Modality Therapy ; Cyclophosphamide ; therapeutic use ; Disease-Free Survival ; Doxorubicin ; therapeutic use ; Female ; Follow-Up Studies ; Humans ; Immunoglobulins ; administration & dosage ; therapeutic use ; Infusions, Intravenous ; Lymphoma, T-Cell, Peripheral ; drug therapy ; therapy ; Male ; Middle Aged ; Neoplasm Recurrence, Local ; Prednisolone ; therapeutic use ; Remission Induction ; Salvage Therapy ; Vincristine ; therapeutic use

This study was aimed to investigate the effect of vcam-1 gene-modified human umbilical cord blood derived stromal cells (CBDSCs) on hematopoietic regulation so as to establish the experimental foundation for further study. The target gene vcam-1 was cloned into the shuttle plasmid with the report gene GFP. The recombinant shuttle plasmid was transformed into BJ5183 bacteria to recombine with backbone vector pAdeasy-l, and the recombinant adenoviral vector ad-vcam-1-gfp was confirmed after transfection with CBDSCs. The results indicated that two fragments of about 9 kb and 2 kb were obtained after digestion of recombinant plasmid pAdTrack-vcam-1 with NotIand XhoI, and single fragment of 600 bp was obtained after amplification with PCR; two fragments of about 31 kb and 4 kb were obtained after digestion of recombinant plasmid pad-vcam-1-gfp with PacI, which suggested a successful homologous recombination. The expression of vcam-1 gene in ad-vcam-1-gfp transfected CBDSCs could be detected by immunocytochemistry, RT-PCR and fluorescent microscopy. It is concluded that the recombinant adenoviral vector ad-vcam-1-gfp has been constructed successfully, and the expression of vcam-1 is up-regulated in CBDSCs transfected by gene ad-vcam-1-gfp.
Adenoviridae ; genetics ; metabolism ; Fetal Blood ; cytology ; Genetic Vectors ; genetics ; Green Fluorescent Proteins ; genetics ; metabolism ; Humans ; RNA, Messenger ; genetics ; metabolism ; Recombinant Proteins ; genetics ; metabolism ; Stromal Cells ; cytology ; Transfection ; Vascular Cell Adhesion Molecule-1 ; genetics ; metabolism