1.Role of acute alcohol poisoning and craniocerebral trauma in the mechanism of death caused by subarachnoid hemorrhage.
Yi-kun TANG ; Meng SHI ; Gui-sheng OU ; Hu ZHAO
Journal of Forensic Medicine 2014;30(5):325-328
OBJECTIVE:
To investigate the relation between the expression of tPA, MMP-2, MMP-9 and AEG-1 in the human brain tissue and the ethanol concentration under the acute alcohol poison, and to analyze the role of alcohol and trauma in the mechanism of death of subarachnoid hemorrhage.
METHODS:
Fifteen real cases were collected in this study. The brain tissues were researched by histological examination and the concentration of ethanol in heart blood were detected. The tPA, MMP-2, MMP-9 and AEG-1 in brainstem, brain and cerebellum were observed respectively by immunohistochemistry.
RESULTS:
In alcohol poisoning groups with or without trauma, the acute alcohol toxicity resulted in the swelling of brain tissues. The tPA, MMP-2, MMP-9 and AEG-1 of brainstem, brain and cerebellum showed high expression in alcohol victims, and the tPA in cerebellum showed no difference. The expression of the MMP-2, MMP-9 and AEG-1 showed good relation with the ethanol concentration in blood (P < 0.05, r > 0.6).
CONCLUSION
The expressions of tPA, MMP-2, MMP-9 and AEG-1 are significant higher in alcohol victims, and expressions of MMP-2 and MMP-9 and AEG-1 have positive correlation with the alcohol concentration. The alcohol has acute toxicity to brain cells.
Alcohol Drinking/adverse effects*
;
Brain
;
Cell Adhesion Molecules/metabolism*
;
Craniocerebral Trauma/etiology*
;
Death
;
Ethanol/poisoning*
;
Heart/drug effects*
;
Humans
;
Matrix Metalloproteinase 2/metabolism*
;
Matrix Metalloproteinase 9/metabolism*
;
Membrane Proteins
;
RNA-Binding Proteins
;
Subarachnoid Hemorrhage/complications*
2.Application of a self-made steel wire guide in the treatment of avulsion fractures of tibial posterior cruciate ligament.
Jing-xiong GUI ; Ju-lun OU ; Xiao-ping WANG ; Xiao-hua ZHU ; Sheng GUO ; Guo-tai XU ; Zhi-cheng DENG
China Journal of Orthopaedics and Traumatology 2016;29(5):468-471
OBJECTIVETo explore the effect of a self-made guiding needle of steel wire in guiding the wire through the tibial tunnel for the treatment of avulsion fractures of tibial posterior cruciate ligament with open reduction and wire fixation.
METHODSFrom February 2011 to June 2014, a total of 22 patients with avulsion fractures of tibial posterior cruciate ligament underwent surgical treatments were analyzed, including 14 males and 8 females with an average age of 35.6 years old (ranged, 17 to 63 years old). According to Meyers classification, 9 patients were classified as type II, 13 patients were classified as type III. All the patients underwent open reduction and wire fixation with medial knee "L" shape approach. A wire guiding needle was used to guide the wire through the tibial tunnel during operation.
RESULTSWith the assistance of wire guidance needles, wires passed through the tibial tunnel rapidly during the operation in all the 22 patients. All the patients were followed up, X-ray imagings 6 months after operation showed the fractures healed well. The average follow-up time in all patients was 6 months (ranged, 6 to 12 months). The averaged Lysholm knee score in 22 knee was 92.7 +/- 3.4. All patients' posterior drawer test were negative.
CONCLUSIONSelf-made wire guiding needle can simplify the operation procedures in which the wires pass through the tibial tunnel, shorten the operation time, reduce the surgical trauma and complications, and be worthy of clinical application.
Adolescent ; Adult ; Bone Wires ; Female ; Follow-Up Studies ; Fracture Fixation, Internal ; Humans ; Male ; Middle Aged ; Posterior Cruciate Ligament ; injuries ; surgery ; Tibia ; injuries ; surgery ; Tibial Fractures ; surgery ; Young Adult
3.Induction of dendritic cells with multidrug resistance from K562/MDR1 cells.
Li-xia SHENG ; Xiao-bao XIE ; Gui-fang OU-YANG ; Yi WANG ; Hui-ling ZHU ; He HUANG
Journal of Zhejiang University. Medical sciences 2011;40(5):489-494
OBJECTIVETo induce the differentiation of K562/MDR1 cells into dendritic cells (DC) with multidrug resistance property.
METHODSK562/MDR1 cells and K562 cells were cultured in the presence of GM-CSF and IL-4 to generate DC and matured by TNF-α. On d14 K562/MDR1-DC and K562-DC cells were harvested and the expressions of CD1a, CD83, CD80, CD86, HLA-ABC and HLA-DR were assessed by flow cytometry (FCM). The antigen presentation function of K562/MDR1-DC and K562-DC was determined by allogenic mixed lymphocyte reaction (Allo-MLR). The expression of P-glycoprotein and the intracellular accumulation of daunorubicin (DNR) were detected by FCM. The sensitivity of K562/MDR1-DC and K562-DC cell to vincristine, adriamycin was measured using MTT assay.
RESULTSBoth K562/MDR1 and K562 cells were differentiated into dendritic cells in the presence of cytokine cocktails, showing the morphologic and immunophenotypic characteristics of DC. K562/MDR1-DC more markedly enhanced proliferation of allogeneic lymphocytes in MLR than K562-DC. High level expression of P-glycoprotein and efflux of DNR were demonstrated in K562/MDR1-DC. K562/MDR1-DC showed multidrug resistance property, with higher IC(50) to VCR and ADM than that of K562-DCs.
CONCLUSIONK562/MDR1 cells can be differentiated into DC with the presence of cytokines, the induced K562/MDR1-DC cells express high level of P-glycoprotein and acquire the multidrug resistance property.
ATP-Binding Cassette, Sub-Family B, Member 1 ; genetics ; Cell Differentiation ; drug effects ; Dendritic Cells ; cytology ; Drug Resistance, Multiple ; Granulocyte-Macrophage Colony-Stimulating Factor ; pharmacology ; Humans ; Interleukin-4 ; pharmacology ; K562 Cells ; cytology ; Transfection ; Tumor Necrosis Factor-alpha ; pharmacology
4.Effects of clotrimazole on the growth of oral squamous cell carcinoma.
Hui LIU ; Yue-Hong WANG ; Zhan-Gui TANG ; Ou-Sheng LIU ; Jie ZHANG ; Gui-Yuan LI
Chinese Journal of Stomatology 2010;45(9):545-548
OBJECTIVEto investigate the effects of clotrimazole on the growth of oral squamous cell carcinoma (OSCC).
METHODSOSCC-25 cells growing in log phase were treated with various doses of clotrimazole. The concentration of IC(50), cell cycle and cell cycle related protein were examined.
RESULTSthe concentration of clotrimazole for inhibiting OSCC was IC(50) 8.51 µmol/L. Clotrimazole induced cell cycle arrest in the G(0)-G(1) cell cycle phase, with a concomitant decrease of cells in the G(2)-M and S-phase. Furthermore, clotrimazole significantly decreased the levels of cyclin D, cyclin E and CDK-4.
CONCLUSIONSclotrimazole inhibits the growth of OSCC.
Carcinoma, Squamous Cell ; pathology ; Cell Cycle ; Cell Cycle Checkpoints ; Cell Division ; Cell Line, Tumor ; Clotrimazole ; pharmacology ; Cyclin E ; Humans ; Mouth Neoplasms ; pathology ; Oncogene Proteins
5.The role of diaphgram fatigue in the death from hanging with bound upper limbs of rabbits.
Fu-Xue JIANG ; Han-Lan JING ; Gui-Sheng OU ; Xin-Biao LIAO ; Yi-Jun ZHANG ; Yan-Geng YU ; Jian-Ding CHENG ; Zhao-Hui LI ; Xiao-Ran GUO
Journal of Forensic Medicine 2006;22(3):165-167
OBJECTIVE:
To explore the role of diaphgram fatigue in the death from hanging with bound upper limbs of rabbits.
METHODS:
Rabbits were hanged with upper limbs bound, then the data of EMGdi were gathered
RESULTS:
By analyzing power spectral of EMGdi in experiment, we compare the ratio change of H/L between pre-experiment and post-experiment. There is a significance decrease of the ratio of H/L, so it indicates that diaphgram fatigue does exist.
CONCLUSION
Diaphgram fatigue plays an important role in the death from hanging with limbs of rabbits bound.
Animals
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Asphyxia/physiopathology*
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Cause of Death
;
Diaphragm/physiopathology*
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Electromyography
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Female
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Forensic Medicine
;
Male
;
Muscle Fatigue
;
Rabbits
6.Evaluation of limited open reduction in the treatment of femoral shaft fractures with intramedullary nail
Jingxiong GUI ; Zhicheng DENG ; Xiaohua ZHU ; Julun OU ; Guotai XU ; Sheng GUO ; Jianzhong XIE ; Jiehao ZHENG
Chinese Journal of Postgraduates of Medicine 2020;43(3):265-270
Objective To evaluate the effect of limited open reduction in the treatment of femoral shaft fractures with intramedullary nail.Methods The clinical data of 39 patients with femoral shaft fractures in the Department of Orthopedic Surgery,Southern Medical University Xiaolan Affiliated Hospital from January 2016 to December 2017 were retrospectively analyzed.All patients were treated with intramedullary nailing.Among them,25 cases were treated with limited open reduction (observation group) and 14 cases were treated with closed reduction (control group).The operation time,fluoroscopy frequency,blood loss volume,infection rate and curative effect were compared between 2 groups.Results The 39 patients were followed up for 7 to 25 (10.3 ± 2.8) months.The operation time and fluoroscopy frequency in observation group were significantly lower than those in control group:(111.4 ± 20.3) min vs.(129.3 ± 21.4) min and (7.0 ± 2.6) times vs.(22.6 ± 7.8) times,but the blood loss volume was significantly higher than that in control group:(454.0 ± 131.4) ml vs.(342.9 ± 120.7) ml,and there were statistical differences (P<0.05 or <0.01);there was no statistical difference in the infection rate and excellent/ good rate between 2 groups (P>0.05).Conclusions Limited open reduction simplifies the operation steps,shortens the operation time,and has fewer fluoroscopy times and less X-ray time.It can achieve the same effect as closed reduction,and can be used as an effective supplementary means when closed reduction equipment and technology are deficient.
7.Effects of parent-child painting and creative crafting therapy on preschool children with autism spectrum disorder and their mothers: a prospective randomized controlled trial.
Gui-Hua LIU ; Ping OU ; Long-Sheng HUANG ; Na-Mei XIE ; Jin-Ling LIN ; Ying-Shuang HE ; Rong-Fang HU
Chinese Journal of Contemporary Pediatrics 2021;23(8):779-785
OBJECTIVES:
To study the effects of parent-child painting and creative crafting therapy on the core symptoms of preschool children with mild-to-moderate autism spectrum disorder (ASD) and the parenting stress and hope level of their mothers.
METHODS:
A total of 56 preschool children with mild-to-moderate ASD and their mothers were divided into an experimental group and a control group using the block randomization method, with 28 pairs in each group. The subjects in the control group received an applied behavior analytic intervention and those in the experimental group received parent-child painting and creative crafting therapy in addition to the intervention in the control group. The intervention time was 20 weeks for both groups. Autism Behavior Checklist (ABC), Social Responsiveness Scale (SRS), Parenting Stress Index-Short Form (PSI-SF), and Herth Hope Index (HHI) were used to evaluate the core symptoms of children and the parenting stress and hope level of their mothers before and after 20 weeks of intervention.
RESULTS:
Forty-nine child-mother pairs completed the study (25 pairs in the intervention group and 24 pairs in the control group). The children in the experimental group had significantly lower scores of social interaction, language, social communication, and social motivation and total scores of ABC and SRS compared with those in the control group (
CONCLUSIONS
The combination of applied behavior analytic intervention with parent-child painting and creative crafting therapy can more effectively improve the core symptoms and social interaction of preschool children with mild-to-moderate ASD, reduce the parenting stress of mothers and improve their hope level.
Autism Spectrum Disorder/therapy*
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Child, Preschool
;
Female
;
Humans
;
Mothers
;
Parent-Child Relations
;
Parenting
;
Prospective Studies
8.Application of pueumatic tourniquet in the operation of lower tibiofibular fracture.
Jing-Xiong GUI ; Guo-Tai XU ; Ju-Lun OU ; Sheng GUO ; Jian-Zhong XIE ; Jie-Hao ZHENG
China Journal of Orthopaedics and Traumatology 2021;34(10):953-958
OBJECTIVE:
To investigate the complications of tourniquet in the clinical application of lower tibiofibular fracture.
METHODS:
From June 2018 to September 2019, 33 cases of closed lower tibiofibular fractures (AO type 43A) were treated with plates and screws and were divided into two groups according to whether pueumatic tourniquet was used:16 cases in the observation group, 13 males and 3 females, aged 18 to 69 (38.8±17.0) years, the operation time after injury was (6.9±1.7) days, and tourniquet was not used during operation. There were 17 cases in the control group, 13 males and 4 females, aged from 21 to 71 (43.8±12.4) years, the operation time after injury was (6.5±1.0) days, automatic pneumatic tourniquetwas routinely used in the operation. The operation time, blood loss, postoperative swelling, pain and other complications were compared between two groups.
RESULTS:
Total of 33 patients were followed up for an average of 15 months. There was no significant difference in operation time and blood loss between two groups (
CONCLUSION
The fracture of lower tibiofibular segment is superficial and easy to be exposed and fixed during operation. In order to avoid tourniquet complications, it is not recommended to use air bag tourniquet routinely or minimize the application time of tourniquet.
Adolescent
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Adult
;
Aged
;
Female
;
Fracture Fixation, Internal
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Fractures, Bone/surgery*
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Humans
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Male
;
Middle Aged
;
Operative Time
;
Retrospective Studies
;
Tourniquets
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Treatment Outcome
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Young Adult