2.Detection of RNA of SARS coronavirus in hospital sewage.
Xin-Wei WANG ; Jin-Song LI ; Ting-Kai GUO ; Bei ZHEN ; Qing-Xin KONG ; Bang YI ; Zhong LI ; Nong SONG ; Min JIN ; Wen-Jun XIAO ; Xiu-Mei ZHU ; Chang-Qing GU ; Jing YIN ; Wei WEI ; Wei YAO ; Chao LIU ; Jian-Feng LI ; Guo-Rong OU ; Min-Nian WANG ; Tong-Yu FANG ; Gui-Jie WANG ; Yao-Hui QIU ; Huai-Huan WU ; Fu-Huan CHAO ; Jun-Wen LI
Chinese Journal of Preventive Medicine 2004;38(4):257-260
OBJECTIVEIn order to explore the existence of SARS coronavirus (Co-V) and/or its RNA in sewage of hospitals administered SARS patients.
METHODSA novel electropositive filter was used to concentrate the SARS-CoV from the sewage of two hospitals administered SARS patients in Beijing, including twelve 2,500 ml sewage samples from the hospitals before disinfection, and ten 25,000 ml samples after disinfection; as well as cell culture, RT-PCR and sequencing of gene to detect and identify the viruses from sewage.
RESULTSThere was no live SARS-CoV detected in the sewage in this study. The nucleic acid of SARS-CoV had been found in the 12 sewage samples before disinfection from both hospitals by semi-nested PCR. After disinfection, SARS-CoV RNA could only be detected from the samples from the 309th Hospital, and the others were negative.
CONCLUSIONIt provides evidence that there is no live SARS-Cov in the sewage from hospitals with SARS patients though SARS-CoV RNA can be detected.
Hospitals ; Humans ; Nucleocapsid ; analysis ; RNA, Viral ; analysis ; Reverse Transcriptase Polymerase Chain Reaction ; SARS Virus ; genetics ; isolation & purification ; Severe Acute Respiratory Syndrome ; virology ; Sewage ; virology
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