1.Flavonoids from Selaginella uncinata.
Mei-ling YI ; Xi-feng SHENG ; Kang-ping XU ; Gui-shan TAN ; Hui ZOU
China Journal of Chinese Materia Medica 2015;40(15):3005-3008
In the current study, nine flavonoids were isolated and purified from 75% ethanol extract of Selaginella uncinata (Desv.) Spring by column chromatographic techniques over macroporous resin, polyamide, silica gel, Sephadex LH-20 and pre-HPLC. On the basis of their physico-chemical properties and spectroscopic data analyses, these compounds were elucidated as cirsimarin (1), nepitrin (2), apigenin-6-C-α-L-arabinopyranosyl-8-C-β-D-glucopyranoside (3), apigenin-6-C-β-D-glucopyranosyl-8-C-α-L-arabinopyranoside (4), apigenin-7-O-β-D-glucopyranoside (5), 2,3-dihydroamentoflavone (6), 4'-O-methylamentoflavone (7), 2,3-dihydro-4'-O-methyl-amentoflavone (8), and 2,3,2",3"-tetrahydron-4'-O-methyl-robustaflavone (9). Compounds 1-5 belong to flavonoid glycosides and were isolated from the genus Selaginella for the first time.
Flavonoids
;
analysis
;
Selaginellaceae
;
chemistry
2.Analysis of heart rate variability in coal miners with pneumoconiosis.
Ling LI ; Ting ZHAO ; You-gui YU ; Chang-hui XU ; Yi-xi WANG ; Shang-jun LIU
Chinese Journal of Industrial Hygiene and Occupational Diseases 2004;22(3):222-223
Aged
;
Coal Mining
;
Electrocardiography
;
Heart Rate
;
Humans
;
Male
;
Middle Aged
;
Pneumoconiosis
;
complications
;
physiopathology
;
Tachycardia
;
etiology
3.Influence of Glucocorticoid on Bone Metabolism in Children with Tubercular Meningitis
feng-lian, ZHU ; gui-ling, FU ; jing, LI ; hui-yin, QIAN ; xue-peng, GUO
Journal of Applied Clinical Pediatrics 2004;0(08):-
0.05).The serum PICP of tubercular meningitis children after 4 neeks glucocorticoid therapy (108.85?46.13) ?g/L was significantly lower than that in control group((154.38)?47.98) ?g/L and glucocorticoid- pretreatment (152.99?44.78) ?g/L (P
4.STUDY ON THE ANTIFUNGAL ACTIVITIES OF ENDOPHYTIC FUNGI ISOLATED FROM SEVERAL PHARMACEUTICAL PLANTS
Gui-Ling LI ; Jian-Feng WANG ; Yao-Jian HUANG ; Zhong-Hui ZHENG ; Wen-Jin SU ;
Microbiology 1992;0(06):-
One hundred and seventy-two strains of endophytic fungi were isolated from Taxus mairei,Cephalotaxus fortunei and Torreya grandis cv.merrillia.The result of the antifungal assay shows that ninety strains of the fungi have antagonism against one or more botanical pathogenic fungi,such as Neurospora sp.,Trichoderma sp.,Fusarium sp.etc.The percentage of antifungal strains to tested strains are as follows:40% Cephalotaxus fortunei,54.2% Taxus mairei,57.1% Torreya grandis cv.merrillia.Thirty-five strains have high antifungal activities,and their inhibition zone diameter is at least 15mm.The active endophytic fungi were identified as 18 genera,most of which belong to Paecilomyces and Fusarium etc.
5.Reduced Incidence and Severity of Collagen-induced Arthritis in Mice Lacking LFA-1
Yi-Nan WANG ; Shi-Yao WANG ; Zhong-Hui LIU ; Xue-Ling CUI ; Gui-Xiang TAI ;
China Biotechnology 2006;0(02):-
Lymphocyte function associated antigen-1 (LFA-1) is a member of integrin family, that plays an important role in the adhesion of lymphocytes with other cells and matrix. To investigate the role of LFA-1 in collagen-induced arthritis (CIA), the incidence of CIA, histological and radiological assessments in the LFA-1 deficient (LFA-1~ -/- ) mice and control mice were examined. LFA-1~ -/- mice and control mice were immunized with 100?g collagen type II(CII) emulsified with an equal volume of Freund’s complete adjuvant (CFA), followed by the booster injection of the same amount of CII in CFA on day 21. Then, clinical, histological and radiological assessments were done. It showed that 57% control mice developed arthritis and apparently changed in the histological and radiological assessment, whereas the all of LFA-1~ -/- mice had the normal histological and radiographic response and none developed arthritis. These results suggeste that LFA-1 is indispensable for the onset of CIA.
6.Analysis of the county-level evaluation results after reaching the goal of eliminating iodine deficiency disorders in Longyan city, Fujian province in 2009
Jian-an, CHEN ; Yong-gui, LAN ; Hui-qin, CHEN ; Xue-ling, RUAN ; Zhi-peng, ZHOU
Chinese Journal of Endemiology 2011;30(2):165-168
Objective To understand the current situation of iodine deficient disorder(IDD) 10 years after achieving the stage goal of eliminating IDD in Longyan city and to evaluate the effect of prevention and treatment measures, and to provide the basis for the development of control strategies. Methods There were 7 counties in the city, and each county(city, district) was as a unit to carry out the inspection for organization and leadership,iodine salt management, monitoring and control, health education (referred to as the four management indicators)according to "The County-Level Assessment and Evaluation Implementation Detailed Rules of Realizing the Goal to Eliminate IDD in Fujian Province". According to the east, west, south, north and middle positions in each county,a village and a primary school were selected. Forty 8 to 10 year-old students in each school were randomly selected to check thyroid and among them 20 students were collected urine samples to determine urinary iodine. Nine townships were selected in the 7 counties of the city and among which 4 administrative villages were selected in each township. Eight edible salt samples from each household in each administrative village were collected to test salt iodine. Goiter was examined by palpation, the level of urinary iodine was examined by arsenic and cerium spectrophotometry, salt iodine was detected by direct titration. Results The average score of the four management indicators was 94.1 in Longyan city. The adjusted goiter rate of children aged 8 - 10 years old was 1.9%. The median of urinary iodine was 278.6 μg/L, among which less than 100 μg/L accounted for 4.57%(32/700), 100 -< 200 μg/L accounted for 24.00%(168/700), 200 - < 300 μg/L accounted for 25.29%(177/700), and higher than 300 μg/L accounted for 46.14%(323/700). The using rate of qualified iodized salt was 98.86%. The coverage rate of iodized salt was 99.50%, the qualified rate of iodized salt was 99.35%, and the rate of non-iodized salt was 0.50%. All the indicators had reached the national standard to eliminate IDD. Conclusions After achieving the stage goal of eliminating IDD, the disease is stable and the effect of control measures are significantly. But the iodine provided has a trend of more than suitable. Therefore, it is reasonable to reduce the current salt iodine content.
7.Outcome analysis of surveillance on iodine deficiency disorders in Longyan City in Fujian Province in the year 2006 and 2007
Hui-juan, CHEN ; Yong-gui, LAN ; Mei-zhu, CHEN ; Jian-an, CHEN ; Hui-qin, CHEN ; Xue-ling, RUAN ; Zhi-peng, ZHOU
Chinese Journal of Endemiology 2009;28(3):319-321
Objective To understand the current situation of iodine deficiency diserders(IDD) in Longyan City and to evaluate the effect of prevention and control measures of IDD in order to provide evidence for formulating prevention and control tactics. Methods During the year of 2006 and 2007, the 30 primary schools were screened by population proportion survey(PPS) from the 7 counties of Longyan City. Forty children aged 8-10 years in each school were randomly selected as a group to examine thyroid, and 7 children in each group were selected to measure the urine iodine and the salt iodine at the same time. The goiter rote, the median urinary iodine, the consumption rate of qualified iodized salt, the iodine salt coverage rate, the rate of qualified iodized salt and the non-iodized rate were detected. Results The goiter rate of children aged 8-10 years old in Longyan City was 0.94%(79/8438). The median urinary iodine was 259.12 μg/L. The consumption rate of qualified iodized salt was 97.86% (1462/1494). The iodine salt coverage rate was 99.46%(1486/1494). The rate of qualified iodized salt was 98.38 (1462/1486), and the non-iodized rate was 0.54% (8/1494). Conclusions All indicators have reached the national standard of eliminating IDD in Longyan City.
8.The solubilization effect of 2-hydroxypropyl-beta-cyclodextrin on paeonolum.
Ling-Yan JIAN ; Yue-Hui YANG ; Ying-Hong XU ; Zhong-Gui HE
China Journal of Chinese Materia Medica 2005;30(15):1154-1156
OBJECTIVETo study the solubilization effect of 2-hydroxypropyl-beta-cyclodextrin(HPCD) on paeonolum at various pH value.
METHODPhase-solubility method was adopted to study the solubilization effect at 25 degrees C and UV spectrohotometer was used to determine paeonolum content.
RESULTSThe apparent solubility of paeonolum was significantly enhanced by increased HPCD concentration. The apparent stability constant of paeonolum compounds was calculated up to 1 425 in which pH was 3 and HPCD concentration was 133.33 mmol x L(-1). The solubility of paeonolum came up to 10 mg x mL(-1).
CONCLUSIONHPCD is an ideal solubilizer for paeonolum.
2-Hydroxypropyl-beta-cyclodextrin ; Acetophenones ; administration & dosage ; chemistry ; isolation & purification ; Drug Stability ; Excipients ; Hydrogen-Ion Concentration ; Paeonia ; chemistry ; Plants, Medicinal ; chemistry ; Solubility ; beta-Cyclodextrins
9.The relationship of neural protection with expression of p-CREB and c-Fos in the hippocampus of neonatal rate after hypoxic-ischemia brain damage and reperfusion.
Guang-li REN ; Ling WANG ; Ying LIU ; Bao-xi WANG ; Zi-wei GAN ; Mao-gui WANG ; Yan-ping HUI
Chinese Journal of Pediatrics 2003;41(5):367-369
Animals
;
Animals, Newborn
;
Cyclic AMP Response Element-Binding Protein
;
analysis
;
Hippocampus
;
blood supply
;
chemistry
;
pathology
;
Hypoxia-Ischemia, Brain
;
physiopathology
;
Immunohistochemistry
;
Proto-Oncogene Proteins c-fos
;
analysis
;
Rats
;
Rats, Sprague-Dawley
;
Reperfusion Injury
;
physiopathology
;
Time Factors
10.The effect of prolactin on the expression of matrix metalloproteinase-9 in the synovium of adjuvant arthritis rats.
Yong-Feng GONG ; Gui-Lan WANG ; Hui-Ling DIAO ; Bao-Yui LI ; Hui ZHANG
Chinese Journal of Applied Physiology 2008;24(1):95-98
AIMTo determine the exact roles of prolactin (PRL) in the pathogenesis of rheumatoid arthritis (RA) and supply experimental basis for clinical treatment of RA, and to investigate the expression of matrix metalloproteinase-9 (MMP-9) in the synovium of adjuvant arthritis rats.
METHODSForty rats were divided into four groups (n = 10): (1) Normal control group (group A); (2) Adjuvant arthritis control group (group B); (3) Hyperprolactinemic adjuvant arthritis group (group C); (4) Hypoprolactinemic adjuvant arthritis group (group D). The content of PRL in the serum was detected by radio-immunoassay method. The activity of MMP-9 was analyzed by gelatin zymography. The alteration of MMP-9 immunoreactivity were investigated by means of immunohistochemistry in the synovium of all groups. The expressions of MMP-9 were investigated by Western blot in the synovium of all groups.
RESULTSCompared with group A, the activity and expression of MMP-9 of group B in the synovium were highly increased. The activity and expression of MMP-9 in the synovium were the most distinctive in group C. Compared with group B, the activity and expression of MMP-9 in the synovium were decreased in group D, but still higher than group A.
CONCLUSIONThe present results indicated that PRL might involved in the pathogenesis of RA by regulating the secretion of MMP-9 in the synovium.
Animals ; Arthritis, Experimental ; metabolism ; Arthritis, Rheumatoid ; physiopathology ; Male ; Matrix Metalloproteinase 9 ; genetics ; metabolism ; Prolactin ; blood ; physiology ; Random Allocation ; Rats ; Rats, Wistar ; Synovial Membrane ; metabolism