OBJECTIVEThis study was designed to investigate the influence of journals indexed by Science Citation Index (SCI) on Chinese medical journals.

METHODArticles on medicine written by Chinese and the journals that published these articles from 2000 to 2009 were searched using Science Citation Index Expanded (SCI-E) database, and the status and variation tendency of the impact factors (IF) of these journals were analyzed. Data of articles on medicine included Chinese Scientific and Technical Paper and Citations Data (CSTPCD) from 2000 to 2008 were searched (the data of 2009 have not been released). The included articles and the time-dependent changing profile were studied. These outcomes were evaluated as the fixed base relative or link relative when compared with the data of 2000 or those of last year, respectively. Geometric mean was used when mean increase was calculated and IF distribution was described with median.

RESULTTotally 3774 articles from China were published by journals indexed by SCI-E in 2000, and the number of articles published by Chinese authors increased every year. In 2008, 16 714 articles were indexed by SCI-E, 442.87% higher than those of 2000. The increment was 161.54% higher than that of articles published in the journals indexed by CSTPCD (281.33%) during the same period. From 2000 to 2009, the geometric mean of increase in the number of published articles from China in journals indexed by SCI-E was 20.87% but it was 18.21% in CSTPCD. From 2000 to 2009, the median of IF of SCI-E indexed journals that published Chinese medical articles was 1.866, 2.073, 2.390, 2.702, 2.409, 2.496, 2.380, 2.218, 2.280 and 2.331, respectively, and they did not increase or even decreased.

CONCLUSIONThe number of the articles indexed by SCI-E increased year by year, much faster than that of CSTPCD. However, it does not necessarily mean the increase in impact.

Bibliometrics ; China ; Journalism, Medical ; Periodicals as Topic ; statistics & numerical data

OBJECTIVETo compare the contents of iridoid in the different processed products from Gardenia jasminoides.

METHODContents of geniposide and genipin gentiobioside in products were determined simultaneously by HPLC. A kromasil C18 column at 35 degrees C was used with the acetonitrile-0.3% formic acid anhydrous (12:88) as the mobile phrase. The detection wavelength was set at 238 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the two components was good with that the relative coefficients (r) were over 0.9994 and the average recoveries were 101.8%, 99.1%, respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. The results showed contents of two components are difference in the different processed products.

Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; Gardenia ; chemistry ; Iridoids ; chemistry

OBJECTIVETo compare the contents of diterpenoid pigments in the different processed products of Gardenia jasminoides.

METHODThe separation of Crocin 1, Crocin 2, Crocin 3, Crocetin were determined simultaneously by HPLC on a kromasil C18 column at 35 degrees C with the m methanol-acetonitrile-0.3% formic acid anhydrous in gradient elution as the mobile phrase. The detection wavelength was set at 440 nm and the flow rate was 1.0 mL x min(-1).

RESULTThe obtained linearity of the four components was better over 0.9995 and the average recoveries were 97.77%, 100.05% , 98.40%, 101.02%, respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. The results showed that the remarkable variation regulations appear among the different processed products.

Carotenoids ; chemistry ; Chromatography, High Pressure Liquid ; Diterpenes ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Gardenia ; chemistry ; Reproducibility of Results

OBJECTIVETo compare the contents of chromones in the different isolation and purification procedures from Saposhnikovia divaricata.

METHODThe separation of six chromones was determined simultaneously by HPLC on a Kromasil C18 column at 35 degrees C with the methanol-0.1% phosphoric acid in gradient elution as the mobile phrase. The detection wavelength was set at 254 nm and the flow rate was 1.0 mL x min(-1).

RESULTAll the obtained correlation coefficient of the six components was over 0.9995 and the average recoveries were not less than 97% (RSD <5%), respectively.

CONCLUSIONThe method is simple, accurate with good reproducibility. Results showed the better preparation procedure is water-decoction and ethanol-precipitation with resin separation and silica gel purification.

Apiaceae ; chemistry ; Chromones ; analysis ; isolation & purification ; Plants, Medicinal ; chemistry

OBJECTIVEThe present study was designed to explore the cited status and its correlated factors of articles published in Chinese Journal of Pediatrics.

METHODArticles published in Chinese Journal of Pediatrics from 2001 to 2010 were searched using Wanfang Medical Online database, and the relationship between cited number and column and funding status were analyzed.

RESULTSTotally 3209 articles were published by Chinese Journal of Pediatrics from 2001 to 2010. Two thousand and seventy-three articles (64.60%) were cited. The total cited number was 18 546 (mean rate: 5.78 per paper). Standard/protocol/guideline was the most often cited column (mean rate: 62.92 per paper). Featured articles including editorials (mean rate: 7.12 per paper), special articles (mean rate: 6.50 per paper) and original articles (mean rate: 7.90 per paper) had higher cited rate. Cited rate of original papers in featured articles was higher than other original articles (mean rate: 6.03 per paper). Those with academic perspectives, such as Opinion/Debate/Discussion, were well cited (mean rate: 7.09 per paper). All of original articles in Rapid Pathway were well cited (mean rate: 16.20 per paper). Mean cited number of grant-supported articles was 4.81 per paper, lower than that of all kinds of papers (mean rate: 5.78 per paper) and non-grant-supported articles (mean rate: 6.06 per paper). However, it was slightly higher than that of articles except Standard/protocol/guideline (mean rate: 4.36 per paper).

CONCLUSIONCited status varies among columns. The invited papers should be increased in number to raise commentary papers and original articles with high quality.Grant-supported or non-grant-supported papers should be reviewed based on the same standard after submission.

Bibliometrics ; Pediatrics ; Periodicals as Topic ; statistics & numerical data

Objective To explore the method of reducing the phenomenon of wet bag in the no-woven packing after sterilization in the central sterile supply .Methods The phenomenon of wet bag in the no-woven operation packing after sterilization was due to the bad absorption of water molecules in the non -woven packaging, movement the non-woven packaging without the given time and poor temperature and humidity in the storage.The improvements were carried out according to the above problems , and the effective method of reducing the phenomenon of wet bagwas summarized .The no-woven packing in the experimental group was packed by no-woven, and filled up a piece of the crepe paper between the instruments and the non-woven and laid the single deck cotton cloth over the sterilization frame in the packaging process .Results The incidence rate of wet bag was decreased from 7.64%to 1.04%three months after improvement.Conclusions Filling up a piece of the crepe paper between the instruments and the non-woven and laying the single deck cotton cloth over the sterilization frame in the packaging process can effectivelyprevent the phenomenon of wet bag in the no -woven operation packing after sterilization .

OBJECTIVETo establish a quick method to detect drug resistance of Mycoplasma pneumoniae (MP) and study the condition of drug resistance in MP infection.

METHODSMP 23S rRNA target gene in throat swab specimens from 200 patients with suspected MP infection was detected by using nested PCR and DNA sequencing. The result of 23S rRNA gene detection was confirmed by MP isolation and drug susceptibility test in vitro for reliability.

RESULTSOf the 200 clinical specimens, 64 were proved to be positive for MP through MP-IgM antibody, MP specific 16S rRNA nested PCR and MP isolation . The 23S rRNA gene was amplified and the gene sequence was compared with MP reference strain in Genbank, 26 were identical to the reference strain, 38 had a point mutation in 23S rRNA. Among them, 35 had A to G mutation at position 2063, 1 had A to C mutation at position 2063 and 2 had A to G mutation at position 2064, the percentage of drug resistance was 59.4%. The sensitivity of the gene detection method was 10(2) ccu/ml and it was confirmed to be reliable by MP isolation and drug susceptibility test.

CONCLUSIONSThe gene detection method could detect MP drug resistant gene directly from clinical specimen, which has the advantages of high specificity, high sensitivity and quickness. It is of great significance for diagnosis of MP infection because MP isolation is difficult and time-consuming.

Adolescent ; Child ; Child, Preschool ; Drug Resistance, Bacterial ; Female ; Genes, rRNA ; Humans ; Infant ; Male ; Microbial Sensitivity Tests ; Mycoplasma pneumoniae ; genetics ; isolation & purification ; Point Mutation ; Polymerase Chain Reaction ; RNA, Bacterial ; genetics ; RNA, Ribosomal, 23S ; genetics

Objective To compare the changes of HPLC peaks of different kinds of Dahuang(Radix et Rhizoma Rhei)decoction pieces，and discuss the change laws of chemical constituents of these decoction pieces in the processing course.Method The procedure of HPLC fingerprint chromatogram was performed on the column of Zorbax Eclipse XDB-C_(18)(4.6 mm ×250 mm，5μm)，using methanol-glacial acetic acid(1.0%)as gradient elution at the temperature of 30℃，and the mobile phase wag at flow rate of 1.0 mL/min.The detection wavelength was at 280 nm.The changes of peak areag of the same constituent in different decoction pieces were compared in 10 batches of 5 kinds of Dahuang decoction pieces.Result Among 24 identified peaks the content'of gallic acid(1)was the highest，and the content of 5 kinds of free anthraquinone including aloe-emodin(20)，rhein(21)，emodin(22)，chrysophanol(23)and physcion(24)reached the highest in prepared Dahuang.The content of 4'hydroxy-phenyl-2-butanone-4'-O-B-D-(6"-galloyl)一glucopyranoside(6)，emodin 8-O-glucoside(17)and aloe-emodin-3-CH_2-O-β-D-glueosidage(18)were the highest in rude Dahuang and the content of other constituents were the highest in wine-treated Dahuang.Conclusion The main factors influencing the chemical constituents of Dahuang decoction pieces before and after processing include the kinds of excipients，temperature and heating time.The higher temperature or longer heating time will reduce the content of anthraquinone glycosides and increase the content of free anthraquinones in varying degrees.

OBJECTIVEIdentify the significance of variants in papillary thyroid carcinoma (PTC), the role of extracellular matrix (ECM), MMPs, cell adhesion molecular (CAM) and cytokine in lymphatic metastasis in PTC and the value of PET-CT in diagnosis of thyroid carcinoma.

METHODSFive hundred and five cases of PTC which had complete medical records and followed up surveys were selected from the files. Clinical biological characteristic of the histological variants was investigated. Sixty cases of PTC were selected. As the important parts of micro ecosystems, ECM, MMPs, CAMs and cytokine were investigated in use of tissue chip and method of immunohistochemistry. In addition, a group of cases of thyroid carcinoma including PTC was analyzed, follicular thyroid carcinoma and medulla thyroid carcinoma (MTC) and their reports of PET-CT as the initiation.

RESULTSThe variants could be divided into three groups in terms of the rate of cervical lymph node metastasis. The high-metastases group included diffuse sclerosing variant, tall cell variant, column cell variant and diffuse follicular variant. The low-metastases group included macrofollicular variant and papillary microcarcinomas. Others are included in the moderate-metastases group. The rate of cervical lymph node metastasis of each group were 83.0% , 55.5% and 34.1% (P < 0. 05), respectively. The 10-year-survival were 75.3% , 95.8% and 100.0% , respectively. The 20-year-survival were 31.2%, 80.3% and 87.5%, respectively. The positive rate of LN, FN, MMP-2, MMP-9, TIMP-2, CD, Integrinbeta-1, ICAM-1, EGFR, TGFR-beta, VEGF-C and E-Cad in metastasis ranged from 51.6% (Integrinbeta-1) to 98.3% (CD), and that in primartumor ranged from 46.7% (FN) to 98.3% (ICAM-1). The expression of E-cad in primartumor was lower than that in normal tissues (P < 0.05). The sensitivity of PET-CT was 100% and the specificity was 85.7%.

CONCLUSIONSThe characteristic has significant difference among the variants. Individual treatment should be performed in terms of different variants. Furthermore, some molecules play an important role in the lymph node metastasis of PTC and may be considered as the focus of future study. In addition, compared with CT and Bus, PET-CT is more sensitive and has its unquestionable advantage as a whole body examination, especially for staging and detecting micro metastases, though it requires a high expense.

Adolescent ; Adult ; Aged ; Carcinoma, Papillary ; diagnostic imaging ; pathology ; Child ; Female ; Humans ; Lymphatic Metastasis ; Male ; Middle Aged ; Positron-Emission Tomography ; Sensitivity and Specificity ; Thyroid Neoplasms ; diagnostic imaging ; pathology ; Tomography, X-Ray Computed ; Young Adult

Adenine ; analogs & derivatives ; therapeutic use ; Adult ; Bone Density ; drug effects ; Creatinine ; blood ; Hepatitis B, Chronic ; complications ; drug therapy ; metabolism ; Humans ; Male ; Organophosphonates ; therapeutic use ; Pain ; etiology ; Phosphorus ; blood