Adult ; Alopecia ; therapy ; Female ; Humans ; Male ; Middle Aged ; Moxibustion ; methods

Adjuvants, Immunologic ; chemistry ; pharmacology ; Anti-Inflammatory Agents, Non-Steroidal ; chemistry ; pharmacology ; Clerodendrum ; chemistry ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Humans ; Phytotherapy ; Urinary Tract Infections ; drug therapy

OBJECTIVETo observe the effects of Bushen Qianggu decoction proliferation and PCNA and Bcl-2 expression.

METHODSSerum containing BQD was made and synovial fibroblasts were separated and cultured and passaged in vitro. Four groups were divided as 20% blank control group, serum containing 20% Tripterygium wilfordii multi-glycosides drug (TWMD), 20% of serum containing high and low of BQD, respectively. Serum containing drugs of different concentration were added into the synovial fibroblasts of the third generation, and then the synovial fibroblasts were cultured continued. The effects of different drugs on synovial fibroblasts and PCNA and Bcl-2 expression were observed.

RESULTSCompared with the control serum, BQD-containing serum promoted the apoptosis of synovial fibroblasts (P < 0.000 1); especially, high dose could inhibit proliferation. The expression of PCNA and Bcl-2 was significantly lower in BQD-containing serum (P < 0.000 1 vs control group).

CONCLUSIONBQD can promote the apoptosis of synovial fibroblasts by improving of expression of PCNA and Bcl-2, which may be one of the mechanisms of BQD in preventing and treating osteoporosis of rheumatoid arthritis.

Animals ; Apoptosis ; drug effects ; Cell Proliferation ; drug effects ; Drugs, Chinese Herbal ; pharmacology ; Female ; Fibroblasts ; drug effects ; physiology ; Proliferating Cell Nuclear Antigen ; analysis ; Proto-Oncogene Proteins c-bcl-2 ; analysis ; Rats ; Rats, Wistar ; Synovial Membrane ; chemistry ; cytology ; drug effects

Objective:To assess the degree of labor pain at the time of intraspinal labor analgesia in women influencing the incidence of cesarean delivery.Methods:Five hundred and ninety patients were enrolled in the study and divided into 3 groups in the light of numeric rating scale (NRS).The woman was with singleton pregnancies, with cervical dilatation of less than 4 cm and vertex fetal presentations.Namely, L group:1-4;M group:5-7;H group:8-10, respectively.The EA procedure was performed at the lumbar spine between 2-3 or 3-4.We inserted a catheter into the epidural space started with 1.0% carbonated Lidocaine to make the volume 5 mL.After the block plane arising, injected a mixture of 0.1% Ropivacaine co-administered with 5 μg of Sufentanil to make the total volume 10 mL in all cases, and connected the patient controlled epidueal analgesia (PCEA) thirty minutes later.The CSEA procedure was started with an intrathecal standard mixture of 0.1% Ropivacaine co-administered with 3 μg of Sufentanil to make the total volume 3 mL in all cases, then connected the PCEA ninety minutes later.To observe and record the NRS score when analgesia was performed.The time of the first stage of labor, the time of the second stage of labor and the way of delivery were recorded in our study respectively.Results:There was no statistically significant differences among the three pain groups in the NRS score after 30 min, dilatation, duration of first stage of labor and duration of second stage of labor, respectively.There was no statistically significant differences among the three pain groups in the incidence of instrumental delivery and cesarean delivery.Conclusions:There is no effect of the degree of labor pain at the time of intraspinal labor analgesia in women on the incidence of cesarean delivery.

OBJECTIVE To observe the effects of Bushen Huoxue Jiangu Decoction drug serum on osteoblast proliferation, differentiation,maturation and mineralization in vitro ,which will provide the theoretical basis for osteoporosis treatment. METHODS Cancellous bone specimens from the femoral head were obtained from patients with femoral neck fracture merger osteoporosis,and the osteoblast cells were seeded and cultured in vitro to establish the osteoblast differentiation model.High, middle and low doses of Bushen Huoxue Jiangu Decoction drug serum were prepared.MTT method,ALP activity assay and a-lizarin red staining were used to observe the proliferation,activity of ALP and the number of mineral node of cultured osteo-blasts in vitro .RESULTS MTT assay displayed different degrees of osteoblast proliferation in high and middle dose groups and showed some dose dependence after 48 h of drug intervention.The activities of ALP in high,middle and low dose groups obviously increased after 72 h of drug intervention.The deposition of calcium salt crystal in alizarin red staining obviously in-creased in high dose group after 72 h of drug intervention.CONCLUSION Bushen Huoxue Jiangu Decoction drug serum can promote osteoblast proliferation,differentiation,maturation and mineralization in vitro .

Objective: To observe the effects of acupuncture plus spinal manipulations on the physical functioning and levels of alkaline phosphatase (ALP), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP) and osteoprotegerin (OPG) in patients with ankylosing spondylitis (AS). Methods: A total of 128 AS cases were allocated into a control group and an observation group using random number table method, with 64 cases in each group. Patients in both groups took sulfasalazine and meloxicam. Patients in the observation group received additional acupuncture plus spinal manipulations. The efficacy, Bath AS functional index (BASFI), Bath AS disease activity index (BASDAI), and the levels of ALP, ESR, CRP and OPG were compared between the two groups after eight weeks of treatment. Results: After treatment, the symptom scores of traditional Chinese medicine in both groups were decreased (all P<0.05), and these scores in the observation group were significantly lower than in the control group (all P<0.05); the VAS, BASFI and BASDAI scores in both groups were decreased (all P<0.05), and these scores in the observation group were significantly lower than in the control group (all P<0.05); and the ALP, ESR, CRP and OPG levels in both groups were decreased (all P<0.05), and these levels in the observation group were significantly lower than in the control group (all P<0.05). The total efficacy rate was 92.2％ in the observation group, versus 78.1％ in the control group, presenting a statistical significance (P<0.05). Conclusion: Conventional medication combined with acupuncture and spinal manipulations can improve clinical symptoms, accelerate the recovery of physical functioning, and reduce the ALP, ESR, CRP and OPG levels.

Objective To investigate the application effect of the integration model of doctors and nurses on high-quality nursing service in the department of radiation oncology.Methods From May 2012 to October 2012,the nursing model,which included the integration of doctors and nurses,the optimization of human resources and the classification management according to nurses'abilities,were implemented in the Second People's Hospital of Sichuan.And the patient satisfaction and the improvement degree of nursing service were are compared before and after carrying out the nursing model.Results The nursing service were evaluated according to doctors,nurses and patients.There were statistically significant different between before and after carrying out the integration model of doctors and nurses at the following contents:nursing abilities [(7.86 ±0.61) vs (6.12 ± 0.47)],communication and coordination skills [(8.42 ± 0.65) vs (6.51 ±0.49)],teamwork abilities [(9.03 ±0.78) vs (6.78 ±0.52)],professional skill and care [(9.11 ±0.80) vs (7.21 ± 0.58) ; (8.15 ± 0.63) vs (6.32 ± 0.45)],and nurse-patient relationship [(7.91 ± 0.62) vs (5.78 ±0.43)],the differences were statistical significant (t =-11.235,-9.987,-8.538,-16.637,-7.651,-6.876;P ＜ 0.05).Compared with before carrying out the integration model of doctors and nurses,patient satisfaction after carrying out the integration model of doctors and nurses improved significantly (x2 =24.335,P ＜ 0.01).Conclusions The implementation of the high-quality nursing service,including the integration of doctors and nurses,the optimization of human resources and the classification management according to nurses'abihties,can help to improve the current status of nursing service and to increase the patient satisfaction and the nursing quality.

OBJECTIVECDH1, encoding E-cadherin, is an important tumor suppressor gene. The present study aims to investigate the association of -160(C-->A) polymorphism in CDH1 gene with susceptibility to gastric cancer in Fujian province.

METHODSOne hundred and two patients from independent families and 101 healthy control subjects were analyzed. Genotype analysis was performed through polymerase chain reaction-based denaturing high performance liquid chromatography. The odds ratios (ORs) and 95% confidence intervals (CIs) were calculated by unconditional logistic regression model.

RESULTSThe frequencies of -160(C-->A) polymorphism CC, CA and AA genotype in case group and controls were 58(56.9%), 38(37.3%)ì6(5.9%) and 55(54.5%), 41(40.6%), 5(5%), respectively. AA genotype did not present a significantly increased risk for gastric cancer (OR=1.12; 95% CI:0.32-3.95). No association was found between A allele and clinicopathological characteristics of gastric cancer.

CONCLUSION-160(C-->A) polymorphism in CDH1 gene promoter region may not be in association with genetic susceptibility to gastric cancer in Chinese population from Fujian.

Adult ; Aged ; Aged, 80 and over ; Asian Continental Ancestry Group ; genetics ; Base Sequence ; Cadherins ; genetics ; China ; Chromatography, High Pressure Liquid ; Female ; Gene Frequency ; Genetic Predisposition to Disease ; genetics ; Genotype ; Humans ; Male ; Middle Aged ; Polymerase Chain Reaction ; Polymorphism, Single Nucleotide ; Stomach Neoplasms ; ethnology ; genetics ; pathology

Study on features of acupoints with resistance test in the past half century is reviewed in this article. Mechanism and technology of the method are introduced as well as its shortcomings. The determination method of signal transmission along meridians with the combination of electrical network theories and practice is advanced. And the result of a series experiments on one meridian at the superficial part of the body are given as well. Thus, it is concluded that the signals of the point-in/point-out and the signals along a non-meridian path with the same distance are significantly different, which gives a verification of the feasibility of the method by using electrical network theories to set out characteristics of signal transmission along meridians dynamically.
Acupuncture Points ; Electrophysiological Phenomena ; Electrophysiology ; instrumentation ; methods ; Humans ; Meridians ; Signal Transduction

OBJECTIVETo evaluate the biomechanical stability of Shen's instrumentation in the management of thoracolumbar fractures.

METHODSThe ranges of movement (ROM) in three dimensions of twelve T(12) - S(3) specimen of fresh adult cadavers were measured sequentially in the states of normal, injured, GSS and SOCON fixation, fatigued GSS and SOCON fixation. Data were then compared.

RESULTSFor the L(2 - 3) segment, the average normal ROM in flexion was 8.3 degrees. In the established spondylolisthesis models, the ROM was 14.7 degrees. After fixation with SOCON, the ROM was decreased to 3.0 degrees, indicating that SOCON fixation can produce good stability. The ROM after fatigue test was 3.6 degrees, significantly lower than that of the normal state, indicating that SOCON still produces good stability after fatigue. For the L(4 - 5) segment, the average normal ROM in flexion was 8.6 degrees. In the established spondylolisthesis models, the ROM was 13.7 degrees. After GSS fixation, the ROM was decreased to 3.2 degrees, significantly lower than that in the normal state, indicating that GSS fixation can produce good stability. ROM after fatigue test was 3.7 degrees, significantly lower than that of the normal state, indicating that GSS can produce good stability after fatigue. The ROM measurements in the other five directions were similar to those for flexion described above.

CONCLUSIONGSS produces good stability for spondylolisthesis, good fatigue resistance, and can be used in pedicle screw revision surgery. There were no significant differences between GSS and SOCON.

Adult ; Biomechanical Phenomena ; Cadaver ; Humans ; Internal Fixators ; Joint Dislocations ; surgery ; Lumbar Vertebrae ; injuries ; Male ; Range of Motion, Articular ; Spinal Fusion ; instrumentation ; methods ; Spinal Injuries ; physiopathology ; surgery ; Thoracic Vertebrae ; injuries ; Young Adult