OBJECTIVETo investigate the relationship between lamivudine-resistant mutants of hepatitis B virus (HBV) and serum HBV DNA loading before antiviral therapy.

METHODSThis study involved 106 patients with hepatitis B receiving lamivudine treatment for an average of 32 months (rang 12-48 months). Serum HBV DNA loadings were measured with PCR before and every 4 to 6 months during lamivudine therapy. HBV YMDD mutants were detected using mismatched PCR-restriction fragment length polymorphism (PCR-RFLP) during lamivudine treatment.

RESULTSHBV DNA loading was significantly higher in patients infected with HBV YMDD mutants during lamivudine therapy than those infected with HBV without YMDD mutation.

CONCLUSIONHigh viral loading in hepatitis B patients before treatment is associated with high likeliness of HBV YMDD mutation during lamivudine treatment. HBV DNA loading may be indicative for the occurrence of YMDD mutation during lamivudine therapy.

Antiviral Agents ; pharmacology ; DNA, Viral ; blood ; Drug Resistance, Viral ; genetics ; Female ; Hepatitis B ; blood ; Hepatitis B virus ; drug effects ; genetics ; physiology ; Humans ; Lamivudine ; pharmacology ; Male ; Middle Aged ; Mutation ; Viral Load ; genetics

OBJECTIVETo study the regulation of luteolin on spleen cells and sarcoma S180 cells in normal ICR mice.

METHODSSpleen cells and S180 cells were incubated with different concentrations of luteolin (50, 100, 200, and 400 μmol/L). The effect of luteolin on spleen cells and sarcoma S180 cells was determined by MTT assay. The apoptosis was detected using propidium iodide staining flow cytometry. Intracellular reactive oxygen species (ROS) was determined by flow cytometric analysis. Activities of free radicals scavenging were determined by hydroxyl radical and DPPH tests.

RESULTSCompared with the solvent control group, 200 and 400 μmol/L luteolin increased the spleen cells viability (P < 0.05). Luteolin at 100, 200, and 400 μmol/L decreased activities of S180 cells (P < 0.01). The proportion of sub-G1 phase spleen cells was reduced after treated with 200 and 400 μmol/L luteolin (P < 0.05). The proportion of sub-G1 phase S180 cells was elevated after treated with 200 and 400 μmol/L luteolin (P < 0.05). Compared with the solvent control group, levels of intracellular ROS in spleen cells of ICR mice all increased; levels of intracellular ROS in S180 cells all decreased after treated with 50, 100, 200, and 400 μmol/L luteolin (P < 0.05). Luteolin scavenged hydroxyl radical and DPPH in a dose dependent manner.

CONCLUSIONLuteolin had bilateral regulation on viability and apoptosis of spleen cells and S180 cells (promoting the viability of spleen cells, inhibiting apoptosis of spleen cells, inhibiting the viability of S180 cells, and promoting apoptosis of S180 cells), which was worth further study and exploration.

Animals ; Apoptosis ; Apoptosis Regulatory Proteins ; metabolism ; Cell Survival ; Luteolin ; metabolism ; Mice ; Mice, Inbred ICR ; Reactive Oxygen Species ; Sarcoma ; Spleen ; metabolism

Objective To explore the efficacy and the clinical practice value of rehabilitation nursing to hepatolenticular degeneration patients with dysarthria. Methods 136 hepatolenticular degeneration patients with dysarthria in hospital were enrolled in this study, and they were randomly assigned to rehabilitation nursing group and usual nursing group. Both groups were treated formally by sodium dimercaptosulphonate, the 68 patients of usual nursing group were given general nursing, the 68 patients of rehabilitation nursing group were given general nursing and rehabilitation nursing of dysarthria. Frenchay dysarthria measurement, Phonolaryngeal graph,Microspiro, Activity of Daily Living Scale and WHOQOL-BREF were used to evaluate and compare the therapeutic effect after 8 weeks. Results The total effect rate of rehabilitation nursing group was 95.6％, and usual nursing group was 72. 1％, and the difference was significant (X2 = 13.88, P ＜ 0. 01). MPT in rehabilitation nursing group was different before and after treatment [(6. 12 ± 5.36) s vs (8.94 ± 6.43) s, t =2. 78, P ＜0. 01] and FVC was also different (t =2.46, P ＜0.05). The MPT and FVC in the two group were different (P ＜ 0. 01). WHOQOL-BREF results have reach the statistically significance in the two group after treatment (P ＜ 0. 01 or P ＜0. 05). Conclusions Dysarthria is a common language barrier of hepatolenticular degeneration; it seriously affects the physical and mental health of patients. Early speech rehabilitation training,with the treatment of hepatolenticular degeneration patients by sodium dimercaptosulphonate, can greatly promote sound organ function recovery, can obviously improve the speech function of patients and can improve daily life ability and the life quality of them, it has high value of clinical applications.

Objective To explore the effects of psychological intervention on the mental status and life quality of hepatolenticular degeneration patients during the perioperative period of splenectomy.Methods 64 cases hepatolenticular degeneration patients during the perioperative period of splenectomy were randomly divided into two groups,the intervention group and the control group,each with 32 cases.Both groups received conventional comfortable nursing,and the intervention group had psychological intervention for eight weeks in addition.Symptom checklist (SCL-90) was used to assess their changes of mental health,while the World Health Organization Quality of Life Scale (WHOQOL-100) was used to assess their quality of life before and after psychological intervention.Results Before psychological intervention,there was no statistically siguificant difference between two groups' SCL-90 scores (P ＞ 0.05).After eight weeks of psychological intervention,the sores of somatization,force,interpersonal relationship,depression,anxiety,hostility,paranoid and psychotic factors in SCL-90 of the intervention group were respectively (1.26 ± 0.43),(1.72 ± 0.44),(1.74 ± 0.45),(1.59±0.43),(1.58±0.52),(1.66±0.74),(1.44±0.57),(1.61±0.68),(1.51±0.43),all of which were lower than those of the control group [(1.60 ± 0.49),(2.06 ± 0.50),(1.74 ± 0.45),(1.85 ±0.49),(1.90±0.55),(2.07±0.66),(1.71 ±0.57),(1.95 ±0.64),(1.82 ±0.56)],and the differences were statistically significant (t =2.950,2.888,2.466,2.256,2.386,2.339,2.060,2.484,respectively; P ＞ 0.05).Before psychological intervention,there was also no statistically significant difference between two groups' WHOQOL-100 scores (P ＞ 0.05).And after eight weeks of psychological intervention,the score of every dimension was higher in the intervention group than in the control group,and the differences were statistically significant (t =4.401,4.694,3.242,5.410,4.576,4.847,3.834,respectively;P ＜0.01).Conclusions Psychological intervention can effectively improve the mental health and quality of life of hepatolenticular degeneration patients during the perioperative period of splenectomy,and thus should be strengthened.

This study was aimed to investigate the expression of SDF-1 mRNA in SDF-1 cDNA-modified human bone marrow mesenchymal stem cells (hBMSCs) before and after transfection. The hBMSCs were isolated, cultured and identified, the SDF-1-pIRES2-EGFP eukaryotic expressing vector was constructed, and then the hBMSCs were transfected with the vector encapsulated by lipofectamine 2000. The transfection efficiency was measured by observing the expression of green fluorescence protein and detecting the mRNA by RT-PCR. The results indicated that the expression of SDF-1 mRNA increased by about 20% after hBMSCs were transfected instantaneously by SDF-1-pIRES2-EGFP. It is concluded that SDF-1 cDNA eukaryotic expression vector can be instantly transfected into hBMSCs by lipofectamine 2000, but the efficiency was too low to obtain enough steady transferred hBMSCs. Other procedures should be trialed to improve the transfection efficiency.
Bone Marrow Cells ; cytology ; Chemokine CXCL12 ; genetics ; metabolism ; DNA, Complementary ; genetics ; Genetic Vectors ; Humans ; Mesenchymal Stromal Cells ; cytology ; RNA, Messenger ; genetics ; metabolism ; Transfection

OBJECTIVETo investigate the chemical constituents of Oldenlandia diffusa.

METHODThe column chromatography with polyamide Sephadex LH -20, silica gel as packing materials and HPLC, were used to separate and purify the chemical components. The structures were elucidated on the basis of physicochemical properties and spectral data.

RESULTNine compounds were isolated and identified as 2, 6-dihydroxy-1-methoxy-3-methylanthraquinone (1), 2-hydroxy-1-methoxy-3-methylanthraquinone (2), 2-hydroxy-3-methylanthraquinone (3), quercetin-3-O-[2-O-(6-O-E-sinapoyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (4), quercetin-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-glucopyranoside (5), kaempferol-3-O-[2-O-(6-O-E-feruloyl)-beta-D-glucopyranosyl]-beta-galactopyranoside (6), quercetin-3-O-(2-O-beta-D-glucop-yranosyl)-beta-D-glucopyranoside (7), rutin (8) and quercertin (9).

CONCLUSIONCompounds 1 and 8 were obtained from this plant for the first time, and compound 1 was a new compound.

Anthraquinones ; chemistry ; isolation & purification ; Molecular Conformation ; Molecular Structure ; Oldenlandia ; chemistry ; Plants, Medicinal ; chemistry ; Quercetin ; chemistry ; isolation & purification ; Rutin ; chemistry ; isolation & purification

OBJECTIVETo sum up the clinical experience of the diagnosis and treatment of intracerebral infiltration by monoclonal plasmacytoid cells in Waldenstrom's macroglobulinemia(Bing-Neel syndrome).

METHODSThe clinical data of the diagnosis and treatment of a case of Bing-Neel syndrome was analyzed.

RESULTSA 56-year-old male was diagnosed as Waldenstrom's macroglobulinemia one year ago, and presented with persistent headache during the treatment period. Magnetic resonance imaging showed a high intensity area on T2-weighed images in the right frontal lobe which was well enhanced by gadolinium-diethylenetriaminepenta-acetic acid. Infiltration of neoplastic cells was confirmed by biopsy. Immunohistochemical examination showed that mature plasmacytoid cells in the cerebral parenchyma were immunoglobulin M positive.

CONCLUSIONInfiltration in CNS (Bing-Neel syndrome) is uncommon in Waldenstrom's macroglobulinemia. As there is no effective therapy for this Bing-Neel syndrome, combination of radiation and chemotherapy should be considered for this situation.

Brain ; pathology ; Humans ; Male ; Middle Aged ; Neoplasm Invasiveness ; Waldenstrom Macroglobulinemia ; pathology

Blood Coagulation Disorders ; drug therapy ; Child ; Child, Preschool ; Drug Therapy, Combination ; Female ; Heparin ; administration & dosage ; Humans ; Male ; Nephrotic Syndrome ; blood ; complications ; urine ; Urokinase-Type Plasminogen Activator ; administration & dosage

The gene encoding amorpha-4, 11-diene synthase was cloned from Artemisia annua L. Other two genes encoding the FPP synthase (FPPS) and HMG-CoA reductase (HMGR) were cloned from Saccharomyces cerevisiae. The cloned cDNAs were confirmed by DNA sequencing. Two expression vectors were constructed, one is named pGBT9/A/HMG/FPP harboring genes for HMG-CoA reductase and FPP synthase and the other is pYeDP60/G/AS, containing the gene encoding amorpha-4,11-diene synthase. Two kinds of engineered yeast were constructed: the first was named WHT [AS], which contained the plasmid pYeDP60/G/AS; the second was WHT [HMG + FPP + AS], in which the vectors pGBT9/A/ HMG/FPP and pYeDP60/G/AS were introduced by cotransformation mediated with LiOAc and PEG4000. The positive clones were identified for further fermentations. The samples from fermentations were analyzed by GC-MS for amorpha-4,11-diene. The results show that engineered yeasts could produce amorpha-4,11-diene. Moreover, the amorpha-4,11-diene production of WHT[ HMG + FPP + AS] and WHT[ AS] were 23.6 mg x L(-1) and 10 microg x L(-1), respectively. Its concentrations were reported as equivalents of valencene. The results showed the copy number increase of HMGR and FPPS genes can improve the production of amorpha-4, 11-diene in the fermentation of engineered yeasts.
Alkyl and Aryl Transferases ; biosynthesis ; genetics ; Artemisia annua ; enzymology ; genetics ; Fermentation ; Gene Dosage ; Genes, Plant ; Genetic Engineering ; methods ; Genetic Vectors ; Geranyltranstransferase ; genetics ; metabolism ; Hydroxymethylglutaryl CoA Reductases ; genetics ; metabolism ; Saccharomyces cerevisiae ; genetics ; metabolism ; Sesquiterpenes ; metabolism

To study the role of hematopoietic microenvironment abnormality in development of minimal residual disease and its mechanism, the viability of HL-60 cells was investigated by means of bone marrow stromal cell culture system or co-culture system of bone marrow stromal cell with HL-60 cells and idarubicin (IDA), flow cytometry and ELISA. The results showed that viability of HL-60 cells gradually decreased along with the increase of IDA dose and prolongation of culture time. Amount of HL-60 cells co-cultured with leukemia bone marrow stramal cells was significantly increased as compared with that of the control (P < 0.05). Bone marrow stromal cells or stromal cell conditioned medium reduced the effect of IDA on HL-60 cells in culture. In conclusion, leukemia bone marrow stromal cells contribute to increasing resistance of HL-60 cells to chemotherapeutic agents, and play some role in developing minimal residual disease.
Bone Marrow Cells ; physiology ; Cell Survival ; drug effects ; Coculture Techniques ; Dose-Response Relationship, Drug ; Drug Resistance, Neoplasm ; HL-60 Cells ; drug effects ; Humans ; Idarubicin ; pharmacology ; Stromal Cells ; physiology