1.Neonatal ulcerative colitis in a case.
Hui ZHANG ; Gui-nan LI ; Xin-hui LIU
Chinese Journal of Pediatrics 2009;47(5):393-394
3.Bacteriology and Clinical Analysis of Severe Pneumonia in Infants and Young Children in Pediatric Intensive Care Unit
hai-yan, LUO ; yi-min, ZHU ; gui-nan, LI ; zheng-hui, XIAO ; xian-bin, LI
Journal of Applied Clinical Pediatrics 2006;0(16):-
0.05);Compared to Gram-negative bacilli group of sputum culture,Gram-positive cocci group had significant diffe-rence in the incidence of gastrointestinal dysfunction and microcirculatory disorders(Pa
4.An analysis on influencing factors of job burnout for nurses from infectious disease hospital
Wen-Li SHEN ; Li-Ping ZHENG ; Gui-Lan DONG ; Rui-Nan LAI
Journal of Preventive Medicine 2016;28(12):1218-1220,1225
Objective To learn the current status of job burnout and influencing factors for nurses from infectious disease hospital.Methods The Chinese version MBI -HSS was used to survey 218 nurses from infectious disease hospital,and linear regression was used to analyze the influencing facters of job burnout.Results A total of 210 questionairs were cocleted.The incidence of severe occupational job burnout was 22.38%,and the score of emotional exhaustion (EE), depersonalization (DP),personal accomplishment (PA)was(22.82 ±9.98),(6.48 ±5.20),and (35.20 ±8.82), respectively.Regression analysis demonstrated that the main influencing factors were entering the isolation ward, opportunity for infectious diseases ,disinfection damage,fear of occupational exposure and concern on family infection(P <0.05).Conclusion The status of job burnout of nurses in infectious disease hospital is not optimistic.There is a positive relationship with the working environment,occupational exposure.Managers need to explore an effective way to ease the job burnout of nurses,and to stabilize the nursing team.
5.Advances in studies on chemical compositions and pharmacological activities of Arnebiae Radix.
Zhi-lai ZHAN ; Jun HU ; Tan LIU ; Li-ping KANG ; Tie-gui NAN ; Lan-ping GUO
China Journal of Chinese Materia Medica 2015;40(21):4127-4135
This article mainly summarise the results of the chemical compositions and their pharmacological activities of Arnebiae Radix since 1966. The chemistry components isolated from Arnebiae Radix are mainly naphthoquinone, monoterpene phenol and quinone, phenolic acids and their salts, alkaloids, aliphatic and esters. Pharmacological results showed that the chemical compositions and the extracts of Arnebiae Radix have antibacterial, anti-inflammatory, anti-viral, hepatoprotection, antioxidant, anti-tumor and immune function and other activities. This article hopefully to provide a reference for further research, development and utilization of Arnebiae Radix.
Animals
;
Boraginaceae
;
chemistry
;
Drugs, Chinese Herbal
;
chemistry
;
pharmacology
;
Humans
;
Molecular Structure
;
Plant Roots
;
chemistry
6.Influence of isoliensinine on activity of CYP3A in rats.
Journal of Zhejiang University. Medical sciences 2012;41(2):178-182
OBJECTIVETo investigate the influence of isoliensinine (IL) on CYP3A enzyme activity.
METHODSA mixture metabolic system of liver microsome enzymes in vitro was developed. A HPLC method to test the metabolic activity of CYP3A was established with testosterone as a probe. The activities of CYP3A enzymes were measured with different IL concentrations and incubation time with testosterone.
RESULTSIn mixture metabolic system of liver microsome enzymes, the best incubation concentration of testosterone was 200 μmol/L, the best incubation time was 210 min, in this condition the IC₅₀ of IL for CYP3A inhibition was >1 000 μmol/L.
CONCLUSIONNo significant interaction between IL and CYP3A is detected, which indicates that IL might be used with CYP 3A enzyme substrates.
Animals ; Cytochrome P-450 CYP3A ; metabolism ; In Vitro Techniques ; Isoquinolines ; pharmacology ; Male ; Microsomes, Liver ; drug effects ; enzymology ; Rats ; Rats, Sprague-Dawley ; Testosterone ; pharmacology
7.Effects of Stainless Steel and Titanium Oxide in Stimulation of Endothelial Cells Releasing NO and MCP-1
Gui-Cai LI ; An-Sha ZHAO ; Ping YANG ; Nan HUANG
Progress in Modern Biomedicine 2008;8(5):836-838
To investigate the influence of stainless steel(SS)and titanium oxide(Ti-O)thin films on the cytokine released by en-dothelial cells.Ti-O films were prepared by unbalanced magnetron sputtering deposition system(UBMS).Human umbilical vein en-dothelial cells(HUVECS)were isolated and seeded on SS and Ti-O materials.Nitride oxide (NO)and monocytc chemotactic protein one(MCP-1)concentrations were determined in the supematants with enzyme linked immunosorbent assay(ELISA)at 1,3 and 5 days after culture,and the morphology of the cells was observed using scanning electron microscopy(SEM).The results showed that the similar amount of HUVECs was presented in SS and Ti-O culture.But HUVEC had better morphology on Ti-O film than SS.The NO and MCP.1 test also showed that HUVECs on Ti-O film had a better bio-function than SS.It is concluded that Ti-O film as a biomaterial may have a better endothlieal compatibility,which can be used to enhance the endothelialization.
8.Analysis of iodine nutritional status and thyroid function of pregnant and lactating women in urban areas of Wuwei City of Gansu Province
Hong-bo, LI ; Yan-ling, WANG ; Xiao-nan, ZHU ; Yu-gui, DOU ; Li-ping, LIANG ; Xu-dong, REN ; Jing, ZHENG
Chinese Journal of Endemiology 2013;(3):273-275
Objective To find out the iodine nutritional status and thyroid function of pregnant and lactating women in the urban areas of Wuwei City of Gansu Province,and to provide a scientific basis for iodine supplementation.Methods Blood and urine samples of pregnant (52 persons) and lactating women (59 persons) were collected in 2009.Urinary iodine content was measured with arsenic cerium catalytic spectrophotometric.Serum free three triiodothyronine(FT3),free thyroid hormone(FT4),triiodo thyronine(TT3),serum total thyroxine (TT4) and thyroid-stimulating hormone (TSH) were detected with direct chemiluminescence immunoassay.Results The medians urinary iodine of pregnant and lactating women were 274.68,217.88 μg/L.The rates of low urinary iodine (pregnant women below 150 μg/L,lactating women lower than 100 μg/L) were 9.62% (5/52) and 6.78% (4/59).Serum TT3,TT4 levels in pregnant women[(2.48 ± 0.59),(132.18 ± 33.36)nmoL/L] were higher than that in the lactating women[(2.16 ± 0.49),(108.79 ± 28.36)nmol/L,t =-3.123,-3.971,all P < 0.05].Thyroid dysfunction incidence rates of pregnant and lactating women were 17.31% (9/52) and 8.47% (5/59).Thyroid dysfunction was mostly subclinical hypothyroidism.Conclusions Overall iodine nutrition of pregnant and lactating women is in good condition,some individuals have the trend of hypothyroidism.It is necessary to carry out routine monitoring of urinary iodine and thyroid function.
9.Effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property.
Hong-zhi SHI ; Nan-nan GAO ; Yong-zhi LI ; Jing-guang YU ; Quan-chun FAN ; Gui-e BAI ; Bing-mu XIN
Chinese journal of integrative medicine 2005;11(2):132-135
OBJECTIVETo study the effects of L.F04, the active fraction of Lycopus lucidus, on erythrocytes rheological property so as to investigate its mechanism in promoting blood circulation and removing blood stasis.
METHODThe effects of L.F04 (used for treatment for 10 days in different dosages) on deformability, aggregation and membrane liquidity of erythrocytes (MLE) as well as whole blood apparent viscosity (eta(b)) were examined on the basis of rat model of blood-stasis syndrome induced by venous injection of high molecular weight dextran.
RESULTAs compared with the normal control group, the model group's RBC deformability and MLE were lower, and the aggregation of erythrocytes and eta(b) were higher. Compared with the model group, both L.F04 0.612 g/kg and 0.306 g/kg showed significant effect in improving deformability and inhibiting aggregation of red blood cells (RBC) and reducing blood viscosity. The trend of improving MLE was also shown.
CONCLUSIONL.F04 could significantly improve the abnormal rheological property of erythrocytes.
Animals ; Blood Viscosity ; drug effects ; Dextrans ; pharmacology ; Drugs, Chinese Herbal ; administration & dosage ; Erythrocyte Aggregation ; drug effects ; Erythrocyte Deformability ; drug effects ; Hemorheology ; Hemostasis ; drug effects ; Lycopus ; Male ; Rats ; Rats, Wistar ; Space Flight
10.Preparation and immunogenicity identification of artificial antigen for luteoloside.
Bo ZHANG ; Zhi-lai ZHAN ; Li-ping KANG ; Yuan YUAN ; Lu-qi HUANG ; Feng-wei DING ; Tie-gui NAN
China Journal of Chinese Materia Medica 2015;40(22):4359-4363
Lonicerae Japonicae Flos was one of the most widely used traditional Chinese medicine for its special biological activities. The content of luteoloside, one of its major compounds, was an important standard for the quantity control of Lonicerae Japonicae Flos. The major method used for the detection of luteoloside was instrumental analysis. Compared with the ELISA method, instrumental analysis was time-consuming, complex pretreatment and low-throughout. Thus, it was significantly important to develop an enzyme-linked immunosorbent assay (ELISA) for luteoloside analysis. Here, the conjugates of luteoloside-bovine (LG-BSA) and luteoloside-ovalbumin (LG-OVA) were produced as the immunogen and coating antigen by the carbodiimide ( CDI) method, respectively. The conjugation ratio of carrier protein and the hapten in the conjugate were determined by UV-Vis spectrophotometry (UV). LG-BSA conjugate was used to immunize Bal b/c mice to produce antiserum. The titer and specificity of antiserum were detected by ELISA. The conjugation ratio of hapten and carries protein were 3. 7: 1 (LG-BSA) and 1. 0: 1 (LG-OVA). The antiserum titer was higher than 2 000 with the linear range of 18.4-4 852.4 μg x L(-1), R2 = 0.988 4 and IC50 = 298.7 μg x L(-1). The result showed that the conjugate antigen LG-BSA was synthesized successfully and the mice can produce specific antiserum injected with artificial antigen.
Animals
;
Antibodies
;
analysis
;
immunology
;
Antigens
;
chemistry
;
immunology
;
Drugs, Chinese Herbal
;
analysis
;
Enzyme-Linked Immunosorbent Assay
;
methods
;
Female
;
Immunization
;
Lonicera
;
chemistry
;
immunology
;
Mice
;
Mice, Inbred BALB C