Due to the irregular of diet and overfeeding greasy and surfeit flavor closely associated with hyperuricemia disease, the lipid emulsion containing high cholesterol was used to model. To obtain a more stable and sustained animal model for the efficacy evaluation of traditional Chinese herbs, we observed the influence on the serum uric acid of rat induced by the lipid emulsion compared with high purine diet. 36 SD male rats were randomized to the normal control group, high purine diet group and lipid emulsion group respectively. The general behavior, body weight and daily food intake of rats were observed. The orbital blood was taken to separate into the serum and 24 hours urine was collected. The serum indexes such as UA, BUN, Cr, ALT, AST, TC, TG, LDL-c were determined every 2 weeks, and XOD, ADA enzyme activity were determined at the 4th week. The urine indexes such as UA, Cr and Cua/Ccr were determined at the 4th week. After stopping modeling, the serum UA were determined two weeks and four weeks later respectively. At the 2nd week, the body weight and daily food intake of rats in the lipid emulsion group reduced significantly, and the level of serum UA, BUN, Cr, TC, LDL-c, ATL, AST raised significantly meanwhile TG reduced. At the 4th week, the serum UA in high purine diet group did not raise, and the serum XOD raised obviously while ADA did not; the serum UA in lipid emulsion group was higher significantly, and the serum XOD and ADA raised while Cua/Ccr reduced obviously. At the 6th weeks, the serum UA in both the high purine diet group and lipid emulsion group raised obviously. After stopping modeling, the serum UA in lipid emulsion group still maintained a high level at the 2nd week and back to the normal level at the 4th week. Compared with high purine diet, the hyperuricemia model induced by lipid emulsion forms earlierand more stable. It maybe has great value to study the pharmacodynamics of traditional Chinese medicine treatment to hyperuricemia disease. Its mechanism may be related to increasing XOD and ADA enzyme activity which can promote uric acid synthesis, meanwhile inhibiting of uric acid excretion.
Animals ; Diet ; adverse effects ; Disease Models, Animal ; Emulsions ; adverse effects ; metabolism ; Humans ; Hyperuricemia ; metabolism ; Lipid Metabolism ; Lipids ; chemistry ; Male ; Rats ; Rats, Sprague-Dawley

Objective To evaluate the clinical features of cardiovascular disease before dialysis in patients with end-stnge renal disease and receiving peritoneal dialysis (PD), in order to provide evidence for judging the prognosis and mastering the emphasis of nursing observation. Methods The informations of 165 patients with end-stage renal disease who underwent PD were analyzed retrospectively. The patients were divided into the diabetic nephrosis group (Group DN) and the non-diabetic nephrosis group (Group non-DN) according to whether the patients had diabetes or not. The patients were divided into the left ventricular hypertrophy group (Group LVH) and the non-left ventricular hypertrophy group (Group non-LVH) according to whether the left ventricular was hypertrophic or not. The ultrasonic examination of the heart and the examination of related laboratory indexes were conducted one week before the treatment. Results The groups had obvious cardiac disease before dialysis. The major exhibitions included left atrial hypertrophy, left ventricular hypertrophy and interventricular septum hypertrophy. Wherein the injury of heart in Group DN was more serious, and the cardiothoracic ratio and the left atrial dimension obviously enlarged. The proportion of hypertension in Group DN was higher than that in Group non-DN, and the levels of hemoglobin and blood albumin were significantly lower than those in Group non-DN (P<0.05). Conclusions The index changes of the cardiovascular system of PD patients should be inspected, especially those elderly patients with the primary disease of DN. The nursing intervention measures should be adopted early so as to prevent the occurrence of cardiovascular complications in good time.

Objective To investigate the effect of hospitalization reformation for patients with renal biopsy. Methods Patients with renal biopsy were divided into clinical pathway group(n = 200) and traditional group (n =200). The differences in the mean hospitalization days, hospitalization charges, complication rate,understanding of renal disease and satisfaction of the two groups were observed and compared. Results The hospitalization days and hospitalization charges of the clinical pathway group were obviously less than the traditional group. The understanding of renal disease and satisfaction were obviously better than those in the traditional group. There was no significant difference in complication rate between the two groups.Conclusions Reformation of the hospitalization by the application of the clinical pathway can reduce the the mean hospitalization days and hospitalization charges obviously and improve the understanding of renal disease and satisfaction of patients significantly.

Objective To analyze the results of detection on influenza A (H1N1) 2009 virus in Beijing from May 2009 to December 2009 and to understand the epidemiologic characteristics during the pandemic period. Methods The study was conducted from the May 1 to December 27,2009. A total of 101 852 throat swab samples were detected with the real-time RT-PCR assay by the Beijing Network Laboratory. Data was statistically analyzed. Results 9843 samples showed influenza A (H1N1) 2009 positive, with an overall positive rate as 9.66%. In terms of the positive rates, they were 2.85% from May to June, 3.32% from July to August and 8.35% from September to October. The peak month fell in November (29.67%) and December (24.33%). The positive rates among the following subpopulations were: 8.40% among the suspected cases, 4.75% among close contact cases, 11.46% among the influenza-like illness cases and 7.33% among the cluster cases with fever. Positive cases mainly fell in age groups 5-14 and 15-24. The ratio of male to female was 1.5:1.Conclusion During the pandemic period of influenza A (H1N1) 2009, positive cases gradually increased during May to November but slowly decreasing in December.