OBJECTIVE:To determine the contents of secnidazole in secnidazol e tablets by RP-HPLC.METHODS:The chromatographic column was VP-ODS C 18 with acetonirile-0.05mol/L potassium dihydrogen phosphate-triethylamine(15∶85∶0.4)as the mobile phase,the detection wavelength was311nm and the flow rate was0.8ml/min.RESULTS:There was a good linear relationship when the sample size was within the range of0.166?g～0.832?g(r=0.9999),the average re?covery was99.84%(RSD=1.05%,n=15).CONCLUSION:The method is simple and accurate,sensible and reproducible,which can be used for the assaying of both the raw materials and preparations and the quality control of secnidazole tablets.

Objective To construct and identify the transgenic plant vector recombinant pBI-Eg95 plasmid of Echinococcus granulosus. Methods Total RNA was extracted from hydatid cyst protoscoleces of Echinococcus granulosus after sonication. A couple of specific primers were designed on the basis of known sequences of Eg95 gene. The desired gene was amplified by PCR technique from the cDNA, and then was cloned into the plant expression vector pBI121 to construct the recombinant pBI-Eg95 plasmid. The recombinant plasmid was electroporated into Agrobocterium tumefaciens (At) LBA4404 strain. The positive recombinant clones were confirmed by restriction endonuclease digestion and characterized by PCR. Results For RT-PCR, a specific band around 471 bp was amplified. The result of DNA sequencing of Eg95 showed the identity with the published sequence. The same band was obtained by restriction endonuclease digestion and PER from the plasmids of positive recombinant At(rAt). Conclusions The recombinant pBI-Eg95 plasmid was successfully constructed, and it provides the basis to further research of the transgenic plant vaccine of Echinococcus granulosus.

Objective To Investigate the preparation method, the release profile and structure of the polyelectrolyte layer- by-layer coated chitosan-alginate microgels. Methods The cores of the microgels were prepared by a high voltage electrostatic system, and the semipermeable membrane outside the microgel was polyelectrolyte deposits on the core surface through electrostatic attraction. The influences of different ratios of materials on the expansion property and the in vitro cumulative release of the coated microgels were evaluated by a single factor experiment. Results The prepared polyelectrolyte-coated microgels were well-shaped, with a narrow range of diameter distribution. The lag time of in vitro release was 2. 67 h; the release was rapid after lagging, with the cumulative in vitro release being 72% within 3 h. Conclusion Polyelectrolyte layer-by- layer coated chitosan-alginate microgels can release payload in a pulsed fashion in vitro.

Objective To Investigate the in vitro and in vivo release of chitosan-alginate microgels coated layer-by-layer by polyelectrolyte self-assembly. Methods The cores of the microgels were made by gelatinization using electrostatic microencapsulated and coated by polyelectrolytes using electrostatic attraction. The effects of different layers and ratios of polymer on the in vitro lease of FITC-dextran were evaluated. Histrological examination was carried out to observe the in vivo release process by injecting the coated microgels into mice. Results The results showed that alginate and calcium chloride concentrations and polyelectrolyte layers markedly affected the lag time of pulsed release and the relasing speed after lagging. Conclusion The release of microgels coated layer-by-layer by polyllectrolyte can be controlled in vitro and can be observed in vivo; meanwhile, the microgels are safe and have good biocompatibility.

Pulsed drug delivery (PDD), which can be released at well-defined time points as the therapy needs, can decrease the frequency and avoid taking drug at night, thus improving patient compliance. Here we introduce three kinds of programmed PDD systems independent of external chemical triggering; they are divided according to the triggering mechanisms, degradation-triggered PDD, osmotic pressure-triggered PDD, and both degradationi and osmotic pressure-triggered PDD. This paper reviews preparing technique, release mechanisms and influencing factors of the three PDD systems. The release profiles of pulsatile PDD can be regulated for different therapeutic needs, requiring no external triggers; especially that the PDD system triggered by both degradation and osmotic pressure has a bright future.

Nicotinamide N-methyltransferase (NNMT) is a S-adenosyl-L-methionine (SAM) dependent cytoplasm enzyme,which plays a vital role in the biotransformation and detoxification of many drugs and xenobiotic compounds.Recent studies have revealed abnormal expression of NNMT in many tumors,which may contribute to tumorigenesis and tumor development and radiotherapy or chemotherapy resistance.

Gastroparesis is a clinical syndrome characterized by delayed gastric emptying of meal in the absence of mechanical obstruction of gastric outlet. In this article,the pathogenesis,etiology,epidemiology,clinical features, diagnosis and treatment of gastroparesis were reviewed.

Adult ; Asymptomatic Diseases ; China ; Chondromatosis, Synovial ; diagnostic imaging ; pathology ; physiopathology ; surgery ; Hip Joint ; diagnostic imaging ; pathology ; physiopathology ; surgery ; Hospitals ; Humans ; Male ; Tomography, X-Ray Computed

Under the socialist market-oriented economic system, state-owned hospitals need to overcome many problems in their traditional management system. The authors discuss a series of issues, including the property right involved in the reform of the management system of state-owned hospitals; the relationships between nonprofit hospitals run by the government and ways of handling them; the conditions of using the corporation management structure in hospitals; the responsibilities of the government in the framework of medical services and the form in which the government supervises state-owned hospitals. Lastly, the authors put forward suggestions from the perspective of policy-making on the reform of the management system of state-owned hospitals.

The recombinant plasmid pPIC-gpd-bgl-hyg was constructed, which contained GPD2 promotor and terminator from industrial yeasts Saccharomyces cerevisiae, ?-glucosidase gene (BGL1) from Sac-charomycopsis fibuligera and hyg from hygromycin as the selected marker. With the yeast’s high efficiency of homologous integrated, the BGL1 gene was successfully integrated into industrial yeasts S. cerevisiae. The recombined yeast could grow on the cultures with the cellobiose as a sole carbon source, and the ?-glucosidase activity achieved 0.764 U/mL after 48 hours’ cultivation. In the experiments of VHG ethanol fermentation, the cellobiose concentration in broth of recombined yeast was 80% lower than that of indus-trial yeast.