Objective To investigate the clinical efficacy of ICARE rebounder tonometer (RBT) by comparing the measurement results of ICARE RBT,applanation tonometer (AT) and non-contact tonometer (NCT).Methods This study included 100 glaucoma suspect cases (100 eyes) with their day and night intraocular pressure (IOP) measurement in Beijing Tongren Hospital from May to November,2014.IOP of each eye was measured by two experienced examiners using RBT,AT and NCT for three times respectively and the mean values were recorded as the final result.Results IOP was measured by RBT,AT,NCT,the values were (19.1s5.7),(18.9±5.0),(15.2±5.0) mmHg (1 mmHg=0.133 kPa),respectively.The difference between RBT and AT was (0.2±1.8) mmHg,and the difference between NCT and AT was-(3.8±2.6) mmHg.There was no significant difference in the values between RBT and AT,t=-1.136,P＞0.05.There was significant difference in the values between NCT and AT,t=14.714,P＜0.01.The results measured by RBT,NCT and AT had correlation,r=0.948,0.822;P＜0.01.The relevant value was 0.973 in two examiners with RBT,which consisted with the result with AT.Conclusion RBT meets the clinical needs and can be widely used in clinical work.