Objective: To study the expression of long noncoding RNA (lncRNA) H19 in human prostate cancer tissue and its effect on the glycometabolism and growth of human prostate cancer cells. METHODS: Realtime quantitative RTPCR (qRTPCR) was employed to detect the expression of lncRNA H19 in human prostate tissues from 20 patients with prostate cancer (10 cases of highGleason score prostate cancer ［HGPC］ and 10 cases of lowGleason score prostate cancer ［LGPC］) and another 5 with benign prostatic hyperplasia (BPH). After transfection of H19 siRNA into the DU145 and PC3 prostate cancer cells, the growth of the cells and the H19 expression in the cells were determined by MTT and qRTPCR respectively, and the changes in the glycometabolism of the prostate cancer cells were analyzed by measuring the contents of glucose and lactate in the culture medium. Nontransfected and transfected negative vectors were used as blank and negative controls respectively. RESULTS The relative expression of H19 was significantly increased in both the HGPC and LGPC tissues (0.725±0.385 and 2.086±0.542) as compared with that in the BPH tissue (0.210±0.068) (P< 0.01), even higher in the HGPC than in the LGPC tissue (P< 0.01). After transfection of H19 siRNA, the expressions of H19 were remarkably decreased in the DU145 and PC3 prostate cancer cells in comparison with those in the blank control and negative control groups (P< 0.01), and so were the proliferation of and the glucose and lactate levels in the DU145 and PC3 cells (P< 0.01).
Cell Line, Tumor ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Glucose ; metabolism ; Humans ; Lactic Acid ; metabolism ; Male ; Prostate ; metabolism ; Prostatic Hyperplasia ; metabolism ; Prostatic Neoplasms ; metabolism ; pathology ; RNA, Long Noncoding ; genetics ; metabolism ; RNA, Small Interfering ; Transfection

Objective To explore the clinical effects of different types of transurethral resection of prostate in the treatment of benign prostatic hyperplasia(BPH)complicated with diabetes mellitus.Methods Ninety-four patients with BPH complicated with type 2 diabetes mellitus admitted to Taizhou Municipal Hospital from January to December 2021 were randomly divided into control group and observation group,with 47 cases in each group.The control group underwent transurethral vaporization resection of prostate,and the observation group underwent transurethral plasmakinetic resection of prostate.The blood sugar,nerve growth factor(NGF),urodynamic indexes and complications of two groups were compared.Results During the operation,the blood sugar in observation group was significantly lower than that in control group(P<0.05).The rate of NGF positive cells in observation group was significantly lower than that in control group(P<0.05).One month after operation,the maximum urine flow rate and bladder compliance in observation group were significantly higher than those in control group,and residual urine volume was significantly lower than that in control group(P<0.05).Within one month after operation,the incidence of complications in observation group was significantly lower than that in control group(P<0.05).Conclusion Transurethral plasmakinetic resection of prostate for BPH patients complicated with diabetes mellitus can reduce the influence of operation on blood sugar,improve detrusor function and urodynamics,improve the therapeutic effect and reduce the incidence of complications.