Objective To examine the effect of somatostatin in the treatment of adhesive intestinal obstruction by meta-analysis. Methods Researchers extensively searched CNKI, Wanfang, and Pubmed and included articles which met the inclusion criteria.For each included article, researchers extracted the research methodology and the related indicators including gastrointestinal decompression, abdominal distension time, exhaust time and hospitalization time.We selected fixed effect models or random effect models to combine the effect of each variable according to the heterogeneity test results.Results Somatostatin could significantly reduce the amount of gastrointestinal decompression -213.19 mL/d (-252.97,-173.40), shorten the abdominal distension time-1.42 d( -1.71,-1.14), exhaust time-1.39 d( -1.79,-0.98), and the hospitalization time-3.46 d( -4.10,-2.83).Conclusion Somatostatin is effective in the treatment of adhesive intestinal obstruction.

Objective: To study the expression of long noncoding RNA (lncRNA) H19 in human prostate cancer tissue and its effect on the glycometabolism and growth of human prostate cancer cells. METHODS: Realtime quantitative RTPCR (qRTPCR) was employed to detect the expression of lncRNA H19 in human prostate tissues from 20 patients with prostate cancer (10 cases of highGleason score prostate cancer ［HGPC］ and 10 cases of lowGleason score prostate cancer ［LGPC］) and another 5 with benign prostatic hyperplasia (BPH). After transfection of H19 siRNA into the DU145 and PC3 prostate cancer cells, the growth of the cells and the H19 expression in the cells were determined by MTT and qRTPCR respectively, and the changes in the glycometabolism of the prostate cancer cells were analyzed by measuring the contents of glucose and lactate in the culture medium. Nontransfected and transfected negative vectors were used as blank and negative controls respectively. RESULTS The relative expression of H19 was significantly increased in both the HGPC and LGPC tissues (0.725±0.385 and 2.086±0.542) as compared with that in the BPH tissue (0.210±0.068) (P< 0.01), even higher in the HGPC than in the LGPC tissue (P< 0.01). After transfection of H19 siRNA, the expressions of H19 were remarkably decreased in the DU145 and PC3 prostate cancer cells in comparison with those in the blank control and negative control groups (P< 0.01), and so were the proliferation of and the glucose and lactate levels in the DU145 and PC3 cells (P< 0.01).
Cell Line, Tumor ; Cell Proliferation ; Gene Expression Regulation, Neoplastic ; Glucose ; metabolism ; Humans ; Lactic Acid ; metabolism ; Male ; Prostate ; metabolism ; Prostatic Hyperplasia ; metabolism ; Prostatic Neoplasms ; metabolism ; pathology ; RNA, Long Noncoding ; genetics ; metabolism ; RNA, Small Interfering ; Transfection

Objective To analyze the epidemic characteristics of brucellosis in Yilan County, Heilongjiang Province, and to provide theoretical reference and scientific basis for prevention and control of brucellosis. Methods Descriptive epidemiology methods were used to analyze the cases of brucellosis in Yilan County in 2012 - 2016. The epidemiological characteristics of brucellosis in Yilan County were analyzed in time distribution, regional distribution and population distribution. Results The total of reported brucellosis was 385, the average annual incidence rate was 18.33/100 thousand, among them, most cases occurred in 2013, which was 92 cases, the incidence rate was 21.90/100 thousand, and a total of 64 cases were diagnosed in 2014, with the lowest morbidity, the incidence rate was 15.24/100 thousand. The peak of incidence happened from February to July, the number of cases was 269, accounting for 69.87％. The number of cases in other months was 116 cases, which occupied 30.13％of the total cases. In 2012-2016, the disease was spreading to every township and village of Yilan. Yugong village had the highest morbidity, it was 43.50/100 thousand, and there were 87 villagers suffered from this disease in total. Yilan township had the lowest morbidity, it was 5.66/100 thousand, and there were 30 patients. Male had the highest incidence, male and female incidence ratio was 3.64:1.00 (302:83). The peak age of onset was 31-60 years old, accounting for 72.47％ (279/385). After analyzing the incidence of occupational groups, farmer was the highest, accounting for 90.13％ (347/385). Most of brucellosis patients contacted with pigs, cows and sheep, accounted for 56.36％ (217/385). Conclusions There are many cases of brucellosis in Yilan County, and the occupation is mainly farmers. The infection route is mostly in contact with livestock. In the future, in response to the epidemiological characteristics of brucellosis in Yilan County, appropriate preventive measures should be taken to control the local outbreak.

Objective:To investigate the value of multiple parameters and the combined model based on 18F-fluorodeoxyglucose (FDG) PET/MR in the differential diagnosis of benign and malignant pancreatic tumors. Methods:A total of 76 patients (50 males, 26 females, age (45.2±18.0) years) with clinically suspected pancreatic tumor who underwent PET/MR between December 2012 and September 2020 in First Medical Center of Chinese PLA General Hospital were retrospectively selected. All patients had definitive diagnoses. PET/MR sequences included T 1 weighted imaging (WI; convention and contrast enhancement), T 2WI, diffusion weighted imaging (DWI) and 18F-FDG PET sequences. Morphological characteristics and scores of lesions in MRI were evaluated. Parameters including parameters of PET and DWI, as well as parameters derived from histogram analysis of apparent diffusion coefficient (ADC) and standardized uptake value (SUV) (maximum (max), minimum (min), mean, median, standard deviation (SD), skewness, kurtosis, entropy) were measured. Independent-sample t test or Mann-Whitney U test were used for data analysis. PET/MR combined model was measured based on logistic regression analysis. Receiver operating characteristic (ROC) curve analysis was used to calculate the differential diagnosis efficacy of PET/MR multiparameters and combined model for benign and malignant lesions. Results:Among 76 patients, 55 were malignant and 21 were benign. (1) Visual evaluation. The main manifestations of pancreatic cancer were unclear margin, abnormal internal MR signal and enhancement, decreased ADC signal and increased radioactive uptake. The main manifestations of benign tumor lesions of pancreas were clear margin, even signal and enhancement, no reduction of ADC, decreased radioactive uptake. (2) Diagnostic efficacy. Multiparameter model established based on logistic regression analysis included SUV max, SUV SD, ADC entropy and ADC skewness. The efficiency of differential diagnosis for benign and malignant pancreatic tumors were shown as follows: multiparametric diagnostic model>ADC entropy>MR score>SUV max>SUV SD>ADC skewness. The multiparametric diagnostic model had the best diagnosis efficiency with the area under curve (AUC) of 0.86, the sensitivity of 69.1%(38/55), and the specificity of 100%(21/21) ( z=-8.73, P<0.001). Conclusions:MR score and multiple quantitative parameters obtained from 18F-FDG PET/MR can be used to differentiate benign and malignant pancreatic tumors. Compared with independent parameter of PET/MR, multiparametric model can further improve the diagnostic efficiency.

Defining and visualizing the three-dimensional (3D) structures of pharmaceuticals provides a new and important tool to elucidate the phenomenal behavior and underlying mechanisms of drug delivery systems. The mechanism of drug release from complex structured dosage forms, such as bilayer osmotic pump tablets, has not been investigated widely for most solid 3D structures. In this study, bilayer osmotic pump tablets undergoing dissolution, as well as after dissolution in a desiccated solid state were examined, and visualized by synchrotron radiation micro-computed tomography (SR-μCT). In situ formed 3D structures at different in vitro drug release states were characterized comprehensively. A distinct movement pattern of NaCl crystals from the push layer to the drug layer was observed, beneath the semi-permeable coating in the desiccated tablet samples. The 3D structures at different dissolution time revealed that the pushing upsurge in the bilayer osmotic pump tablet was directed via peripheral "roadways". Typically, different regions of the osmotic front, infiltration region, and dormant region were classified in the push layer during the dissolution of drug from tablet samples. According to the observed 3D microstructures, a "subterranean river model" for the drug release mechanism has been defined to explain the drug release mechanism.