Objective To investigate non gonococcal urethritis mycoplasma and chlamydia infection and drug sensitivity status.Methods From June 2014 to December 2014,120 cases of non gonococcal urethritis patients in our hospital were given chlamydia trachomatis,ureaplasma,mycoplasma detection and mycoplasma culture and drug sensitivity test of mycoplasma.Mycoplasma and chlamydia test results were compared between male and female patients.Positive drug sensitivity test results of ureaplasma urealyticum (Uu) and mycoplasma hominis (Mh) were observed.Results Among the 120 patients with positive detection,the proportion of Uu was the highest,up to 41.67％ (50/120);the second was Mh,accounting for 31.67％ (38/120).The detection rate of Uu in women (57.14％)was significantly higher than that in males (28.13％),and the difference was statistically significant (x2 =10.35,P ＜ 0.05).The susceptibility of mycoplasma to pristinamycin susceptible rate highest,Uu and type mycoplasma reached 100.00％;followed by doxycycline,the susceptibility of Uu reached 98.00％ and the susceptibility of Mh to 100.00％;sensitive rates of Uu and Mh to josamycin were 96.00％ and 90.48％.The sensitive rates of Uu and Mh to tetracycline were 92.00％ and 90.48％ respectively.Conclusion The mycoplasma infection was mainly caused by Uu.Clinical treatment of mycoplasma infection can be based on the drug sensitivity test results to reasonably choose antibiotics,and sensitive rates of pristinamycin,doxycycline were higher.