Objective The aim of this study was to investigate the expression of WEE1 in gastric cancer and its influence on prognosis of patients. Methods Seventy-eight patients with gastric cancer were enrolled. According to the presence or absence of lymph node(LN)metastasis,the patients were divided into LN( -)group and LN( +)group. The expression of WEE1 in gastric canc-er tissues was detected by immunohistochemistry and RT-qRCR techniques. The influence of WEE1 expression on LN metastasis and prognosis of gastric cancer were analyzed statistically. Results (1)The positive rate of WEE1 in gastric cancer was 43. 6% ,of which 13 cases were a low expression and 21 cases with high expression. The positive rates of WEE1 in LN( +) group and LN( -) group were 53. 8% and 23. 1% ,respectively. (2)The results of RT-qPCR showed that the mRNA expression of WEE1 in LN( -)and LN ( +)groups was(1. 32 ± 0. 21) and(3. 64 ± 0. 41),respectively,and the difference was statistically significant(P<0. 01). (3) The results of ROC curve showed that the area under curve(AUC)of WEE1 mRNA expression level for LN metastasis of gastric cancer was 0. 806,the sensitivity was 84. 8% ,the specificity was 79. 6% ,and the diagnostic efficacy was good. T stage and LN metastasis were independent risk factors for the expression of WEE1(P<0. 05). (4)Among 78 patients,7 patients were lost to follow-up,the rate of loss of follow-up was 8. 9% ,and 41 patients died within 5 years. Among them,13,10 and 18 died in the WEE1( -)group,low-ex-pression of WEE1 group and high-expression of WEE1 group,respectively. The 5-year survival rates of the three groups were signif-icantly different(χ2 =25. 67,P<0. 001). The survival rate in the low expression of WEE1 group was not significantly different from the high expression of WEE1 group(P>0. 05),but both of them were significantly low the WEE1( -)group(P<0. 05). Conclusion WEE1 has a high positive rate in gastric cancer,and is closely related to patient stage and LN metastasis. The positive expression of WEE1 is a strong signal for poor prognosis.

Objective:To investigate the efficacy and safety of modified endoscopic submucosal multi-tunnel dissection (ESMTD) for superficial circumferential esophageal cancer with an axial length of more than 8 cm.Methods:Data of 79 patients with superficial circumferential esophageal cancer with lesion length of more than 8 cm who were treated in the First Affiliated Hospital of Nanjing Medical University from January 2018 to December 2021 were retrospectively analyzed. Patients were divided into modified ESMTD group (32 cases) and surgery group (47 cases) according to the treatment. The en bloc resection rate, complete resection rate, operation time, hospitalization time, medical expenses, incidence of procedure-related complications of the two groups were compared.Results:The en bloc resection rate in the modified ESMTD group and the surgery group were both 100.0% ( χ2=0.000, P=1.000), and the complete resection rate were 96.9% (31/32) and 97.9% (46/47) ( χ2=0.000, P=1.000), respectively. The operation time in the modified ESMTD group was shorter than that in the surgery group (150.5±17.2 min VS 185.8±15.2 min, t=9.527, P<0.001). The incidence of delayed bleeding [3.1% (1/32) VS 10.6% (5/47), χ2=0.648, P=0.421] and delayed perforation [3.1% (1/32) VS 4.3% (2/47), χ2=0.000, P=1.000] in the two groups were not statistically different. Postoperative C-reactive protein (64.3±6.9 mg/L VS 89.2±7.4 mg/L, t=15.634, P<0.001) and neutrophil levels [(10.1±1.4)×10 9/L VS (13.1±1.2)×10 9/L, t=15.083, P<0.001] were lower in the modified ESMTD group than those in the surgery group. The hospital stay of the modified ESMTD group was shorter than that of the other group (9.2±1.2 d VS 11.5±1.2 d, t=8.363, P<0.001), and the medical expense was less than that of the surgery group (32±3 thousand yuan VS 59±6 thousand yuan, t=26.384, P<0.001). Conclusion:Compared with traditional surgery, modified ESMTD for the treatment of superficial circumferential esophageal cancer with an axial length >8 cm has definite curative effect, safety, short hospital stay, and low medical costs, and can preserve the integrity of the esophagus and improve the quality of life of patients. It has good clinical application value.