Objective In order to study the etiology and the hearing status of the deaf students in Hubei province, a survey was carried out from April 1999 to June 2000.Methods A total number of 813 deaf students in Hubei province were examined with audiometer and investigated through questionnaire. The pedigress analysis was conducted in deaf students with family history.Results The pedigrees of 227 familes with deafness were obtained, the inheritance pattern of 167 families could be ascertained. 232(28.5%) deaf students were diagnosed congenital deafness, 581 (71.5%) students were diagnosed acquired deafness. The degrees of deafness could be ascertained with 359(44.1%) students of profound deafness, 323(39.7%) students of severe deafness, 111(13.7%) students with moderate to severe deafness, 11(1.4%) students of moderate deafness, and 9(1.1%) students of mild deafness.Conclusion The hearing loss of deaf students is very serious, and genetic factor and ototoxic antibiotics were a principal causation in the occurrence of deafness.

Aneurysm, False ; therapy ; Carotid Artery Diseases ; therapy ; Carotid Artery, Internal ; pathology ; Humans

Objective To summarizes the experiences and technique of complex transradial percutaneous coronary intervention (PCI) using sheathless guide catheter (Sheathless Eaucath, ASAHI) for 60 patients with coronary heart disease. Methods Retrospectively analyzed the data from 60 patients received PCI using sheathless guide catheter. Results PCI were performed on a total of 60 patients with complex lesions. Angiographic success was achieved in 95.0%and failed in three patients with CTO. 7 patients were with left main lesions, and 32 patients were with bifurcation lesions(including 7 patients with left main lesions and 2 patients with CTO), and 13 patients were with CTO, and the other 17 patients were with tortuous and calcified lesions. Sheathless guide catheters of 7.5Fr were used for PCI in all patients, and all catheters successfully passed through the radial artery and were put in place. No other vascular complications associated with the use of the catheter occurred. Sheathless guide catheters were respectively JL(8 patients), PB(2 patients), AL(2 patients), and JR(1 patient) in 13 patients with CTO, and were respectively JL(8 patients), JR(6 patients), SPB(2 patients), AL(1 patient) in 17 patients with tortuous and calcified lesions. JL or JR were used in all 32 patients with bifurcation. Conclusions Use of the Sheathless of 7.5 Fr is safe and feasible, and allows complex interventions to be undertaken transradially with a high success rate.

Objective To analyze the clinical characteristics and risk factors of multiple drug -resistant bacteria infection in patients with diabetic foot .Methods The clinical data of 60 patients with diabetic foot were retrospectively analyzed , including diabetic foot infected ( positive group ) and uninfected multiple drug -resistant bacteria(negative group),30 cases in each group.Application of SPSS 17.0 statistical software for infection and clinical characteristics of the single factor analysis , through the Logistics regression analyzed the risk factors of infection . Results A total of 130 strains bacteria were isolated in the positive group ,of which more than 64 strains of drug-resistant bacteria,the detection rate was 49.23％;The top three for staphylococcus aureus (60.00％),enterobacter (53.33％) and verdigris pseudomonas (31.57％).Single factor analysis results showed that age , hypertension between the two groups had no statistically significant differences (all P>0.05);Positive group in the use of antibiotics prior to admission time,ulcerated area,etc.,compared with the negative group the differences were statistically significant (t=1.812, -7.268,all P <0.05).Multiple factors analysis showed that admission ,antibiotics,ulcerated area, osteomyelitis were risk factors of positive infection in patients .Conclusion The risk factor resulted in diabetic foot patients infected multiple drug -resistant bacteria is more ,and the amputation rate of patients with infection group is obviously higher than that of patients who are not infected ,to ensure that the prognosis of patients with diabetic foot level,should strengthen the control of multiple drug -resistant bacteria infection ,thus to better achieve treatment goals .

Objective To evaluate the clinical outcomes of partial lateral patellar facetectomy ( PLPF ) combined with lateral retinaculum release ( LRR ) for treatment of patellofemoral osteoarthritis ( PFOA ). Methods From June 2017 to March 2018, 30 PFOA patients underwent PLPF combined with LRR at Department of Orthopedics and Traumatology, Foshan Hospital of Traditional Chinese Medicine. They were 7 men and 23 women with an average age of 56.4 ± 9.7 years. Their patellar position, patellofemoral joint function, overall knee function, and quality of life were assessed by comparing preoperation and last follow-up in patellofemoral congruence angle ( PFCA ) , lateral patellofemoral angle ( LPFA ) , modified Kujala score, The Western Ontario and Mcmaster Universities Osteoarthritis Index ( WOMAC ) , and SF-12 quality of life scale. Results All the patients were followed up for an average of 7.6 ± 3.4 months ( from 4 to 13 months ). The PFCA was improved from preoperative 22.9°± 7.6°to 12.4°± 4.2°at the last follow-up, the LPFA from preoperative 3.2°± 3.7° to 12.9°± 6.0° at the last follow-up, the modified Kujala score from preoperative 17.1 ± 9.8 to 34.3 ± 5.7 at the last follow-up, the WOMAC from preoperative 14.1 ± 5.2 to 5.9 ± 1.7 at the last follow-up, the stiffness index from preoperative 5.5 ± 3.2 to 2.7 ± 1.2 at the last fol-low-up, daily functional index from preoperative 43.9 ± 9.0 to 25.2 ± 5.4 at the last follow-up, and the SF-12 scores from preoperative 31.3 ± 5.2 to 55.7 ± 6.0 at the last follow-up. All the above comparisons showed a significant difference ( P ＜0.05 ) . Conclusion PLPF combined with LRR is a minimally invasive, easy-to-master and effective knee joint preserving procedure for PFOA as it can significantly relieve joint pain and maximally keep patellar functions.

Colorectal cancer (CRC) is one of the leading causes of death worldwide. Thus, the development of new therapeutic targets for CRC treatment is urgently needed. SGK1 is involved in various cellular activities, and its dysregulation can result in multiple cancers. However, little is known about its roles and associated molecular mechanisms in CRC. In present study, we found that SGK1 was highly expressed in tumor tissues compared with peri-tumor samples from CRC patients. In vitro experiments revealed that SGK1 overexpression promoted colonic tumor cell proliferation and migration and inhibited cell apoptosis induced by 5-fluorouracil (5-FU), while SGK1 shRNA and inhibitors showed the inverse effects. Using CRC xenograft mice models, we demonstrated that knockdown or therapeutic inhibition of SGK1 repressed tumor cell proliferation and tumor growth. Moreover, SGK1 inhibitors increased p27 expression and promoted p27 nuclear accumulation in colorectal cancer cells, and p27 siRNAs could attenuate the repression of CRC cell proliferation induced by SGK1 inhibitors. Collectively, SGK1 promotes colorectal cancer development via regulation of CRC cell proliferation, migration and survival. Inhibition of SGK1 represents a novel strategy for the treatment of CRC.
Animals ; Apoptosis ; Cause of Death ; Cell Proliferation ; Colon ; Colorectal Neoplasms* ; Fluorouracil ; Heterografts ; Humans ; In Vitro Techniques ; Mice ; Repression, Psychology ; RNA, Small Interfering

Objective To understand the demographic characteristics of HIV infected injecting drug users (IDUs) with access to antiretroviral therapy (ART) and to identify the factors influencing their access to methadone maintenance treatment (MMT).Methods Outpatients infected with HIV through injecting drug use were selected from the ART clinics in 2 counties in Yunnan province.They were divided into 2 groups,MMT group and non MMT group.Descriptive epidemiologic analysis was conducted on their demographic characteristics,disease history and high risk behaviors and logistic regression analysis was done to identify the factors associated with the access to MMT.Results Among 635 IDUs (536 males,99 females) surveyed,247 received MMT (38.9％),388 received no MMT(61.1％).The median age was 40.33 years and the median diagnosed HIV infection time was 6.08 years.Multivariate logistic regression indicated that being female (OR=2.40,95％CI:1.00-5.74),educational level of junior high school (OR =3.28,95％CI:1.75-6.14),educational level of senior high school or above (OR =7.10,95 ％CI:1.90-26.62),more than 6 years of diagnosed HIV infection history (OR=3.84,95％CI:2.11-6.98) and HCV positive (OR=6.21,95％CI:3.06-12.58) were the positive factors influencing IDUs' access to MMT.However,being married (OR=0.38,95％CI:0.20-0.72) or being employed (OR=0.01,95％CI:0.00-0.02) were the negative factors influencing IDUs' access to MMT.Conclusion The proportion of HIV infected IDUs who received MMT in ART clinics was still very low in the two counties,targeted intervention measures should be taken to increase the MMT coverage,especially among those who are married or employed.