Obiective To test quality control of anesthesia respirator. Methods Based on Technology Criterion for Anesthesia Respirator's Quality Inspection of headquarters, VT-Plus-HF gas analyzer that made in FLUK Co. As a regulator was detected thoroughly from the equipment appearance and accessories, alarm and safety system, aerate parameter.Conclusion According to equipment quality, usual operation and preventive maintenance, related solutions and suggestions are introduced.

Objective To investigate the inhibitory effect of sodium phenylbutyrate(SPB) combined with gefitinib on NB4 cells.Methods The NB4 cells were divided into 8 groups randomly:control group,PMA group,H-7 group,SPB+PMA group,gefitinib group,gefitinib+PMA group,SPB+gefitinib group.The inhibitory rate of medicine was detected by MTT,the apoptosis of NB4 cells was detected by flow cytometry,the expressions of PKC,CDK,and P53 were detected with Western blotting method.Results The NB4 cells were suppressed obviously.The rate of growth inhibition and proliferation index in SPB+gefitinib group were 92.8% and 5.96%,those in SPB group were 45.1% and 29.6%,and gefitinib group were 61.0% and 35.9%,the differences were significant(P

Objective:To observe the effects of sodium butyrate on the expression of CD86 molecule on acute leukemia cells and explore the mechanisms of action.Methods:The expression of CD86 in NB4,HL-60 and U937 treated by SB or not was assayed by flow cytometric analysis.The alteration of CD86 mRNA was examined by semiquantitative RT-PCR.AUT gel electrophoresis was applied to check the state of histone acetylation.The content of phospho-CREB was assayed by pCREB kit.Results:Up-regulation of CD86 was observed on those cells treated by SB.The levels of CD86 mRNA in SB treated cells were statistically enhanced.The acetylation degrees of SB treated cells were higher than control groups.The contents of phospho-CREB were also raised in SB treated cells.Conclusion:SB can improve the acetylation states of acute leukemia cells,remodel the chromatin which contributes to the binding on DNA of transcription factors,such as CREB and then promote the transcription and expression of CD86.

Objective To investigate the clinical efficacy of unilateral small incision Quadrant channel assisted MIS?TLIF unilateral pedicle screw fixation system in the treatment of degenerative lumbar disease. Methods From January 2011 to December 2013,a total of 56 cases with low back and leg pain were selected in the People′s Hospital of Dongguan,including 25 cases with lumbar disc herniation,18 cases with lumbar tube stenosis,10 cases with discogenic low back pain,2 cases of recurrence after posterior lumbar spine surgery,1 case of recurrence after transforaminal endoscopic surgery. Unilateral pedicle screw fixation was performed in the treatment of MIS?TLIF with expandable pipeline system. VAS and Oswestry dysfunction index scoring system( ODI) were used to evaluate of pain and functional recovery in patients with preoperative and postoperative pain and functional recovery,the Suk method was used to observe the bone graft fusion. Results There were 5 cases of non operative side waist back pain after operation,and the waist circumference and anti?inflammatory pain relief were improved after treatment. One case of postoperative subcutaneous fat liquefaction, was cured by dressing change. One patient with recurrence of MED intraoperatie cerebrospinal fluid leakage,was cured after treatment by the bed,dehydration and others. Other complications such as infection,screw loosening, nerve root injury and other complications had no found. After 1 month,the VAS score from preoperative ( 6. 82 ±0. 92) points fell to (1. 95±0. 55) points,ODI score from preoperative (35. 21±2. 73) points fell to (10. 05 ±1. 72) points, significantly improved compared with the preoperative, the differences were statistically significant( t=36. 775,65. 858,P<0. 05) ,based on the fusion of Suk judgment method,2 cases of patients with possible fusion,the rest were fusion. Conclusion Unilateral small incision under the quadrant assisted MIS?TILF unilateral pedicle nail stick system has obvious advantages in treatment of degenerative lumbar spine disease,as long as we choose to suitable cases and most patients can obtain satisfactory results.

Objective To explore the correlation between miR-25 and FBXO33 in renal cell carcinoma (RCC),and to analyze the relationship with apoptosis and prognosis of renal cell carcinoma.Methods The 511 RCC chip results,from 1998 to 2013,were downloaded from the Cancer Genome Atlas (TCGA)database,and were analyzed for the correlation between miR-25 and FBXO33 by Pearson test.The expression of fluorescein were detected with the FBXO33 3’UTR wild-type,mu-tant and blank control luciferase reporter gene treated by miR-25.The viability of cells transient translated by the miR-25 mimic,siRNA and the controls were detected by CCK8 method.The apoptosis of cells transient translated by the miR-25 mimic,siRNA and the controls were detected by flow cytometry.58 cases with follow-up data were screened from TCGA by expression of FBXO33 negative correlation miR-25.The survival was analyzed between low expression of miR-25 combined with FBXO33 high expression group (n=34)with high expression of miR-25 combined with FBXO33 low expression group (n=24),using Log-rank test and Gehan-Breslow-Wilcoxon test.Results FBXO33 was negatively correlated with miR-25 in RCC tissue (r=-0.161 1,Pearson test).Compared with the control group,miR-25 could reduce the RLU of wild type group to 80.2%±2.6%,the difference was statistically significant (t=6.539,P=0.006).The RLU of mutation group was 103.5%±8.4% compared with that of blank control group,the difference was not statistically significant (t=0.041 3,P=0.968 4),compared with the blank group in 72h for the cell varibility,miR-25 siRNA group were elevated by 32.7%± 3.5%,the difference was statistically significant (P<0.05).The miR-25 mimic group were reduced by 23.3%±1.7%,the difference was statistically significant (P<0.05),and compared with the control group,the early apoptosis rate was de-creased in mimic-miR-25-3p group (8.83 ± 0.09 vs 12.83 ± 0.14),while the difference was statistically significant (t=42.17,P=0.005).The late apoptosis rate was slightly escalated (0.41±0.10 vs 0.33±0.15),while the difference was not statistically significant (t=0.75,P=0.639).Compared with the control group,the early apoptosis rate was increased in siR-NA-miR-25-3p group (19.05 ± 1.64 vs 13.68 ± 0.78),while the difference was statistically significant (t=5.12,P=0.006).But the late apoptosis rate was reduced (0.56±0.10 vs 0.62±0.08),while the difference was not statistically sig-nificant (t=0.83,P=0.376).The survival rate was higher in patients with low expression of miR-25 combined with high expression of FBXO33 (n=34)than that of miR-25 high expression combined with low expression of FBXO33 (n=24),the difference was statistically significant (Log-rank test P=0.025 2,Gehan-Breslow-Wilcoxon test P=0.004 9).Conclusion MiR-25 can inhibite FBXO33 in renal cell carcinoma,improve the cell activity,inhibit apoptosis and reduce the prognosis.

Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell.Methods:The cDNA encoding soluble human Flt3 ligand(FL) was obtained by RT PCR from the TF 1 cell lines and was inserted into eukaryotic expressing vector pIRES1neo between EcoR I and BamH I sites after sequencing,and then COS 7 cells were transfected with the recombinant by liposome.The transcription and expression of hFL in the transfected COS 7 cells were assayed by RT PCR,ELISA and the experiment of the human umbilical blood CD34 + cell multiplication,respectively,at 72 hours after transfection.Results:It showed that hFL cDNA cloned in our laboratory was 546 bp in length encoding soluble human FL which was in accordance with the report previously.FL gene was transcripted in transfectants,and FL protein with obvious biological activity was highly expressed with 251 ng/(10 6 cell?d) in the supernatant of the transfectants.Conclusion:Human FL in the recombinant vector is proved to be expressed in COS 7 cells and obvious biological activity of hFL in the supernatant of the transfectants was detected.

BACKGROUND:Diameter and length of mini-implant have effects on its stability, which has been reported mostly in I and II osteoid, but less in IV osteoid. OBJECTIVE:To optimize the design of mini-implant diameter and length in IV osteoid by a three-dimensional finite element analysis. METHODS:Implant-mandible solid model was established. A 2 N orthodontic force that was perpendicular to the long axis of the implant and at a 30° angle with the distal central axis was applied onto the top of the implant. The implant was designed for different diameters (1.2-2.0 mm) and lengths (6-10 mm). Peak stress and peak displacement of the mandible were mechanicaly assessed, and stress sensitivity variables were analyzed. RESULTS AND CONCLUSION:The stress and displacement of the implant were mainly concentrated in the neck of the implant. The stress of implant-bone interface mainly focused on the contact area of the implant-cortical bone interface, and the stress of the cancelous bone was relatively smal, but the stress of the cortical bone was weakened faster. When the implant length was constant, the implant diameter had a great effect on stress changes, and the stress of bone tissue was reduced with the increase of implant diameter. When the implant diameter was constant, the implant length had no significant effect on the stress of bone tissue. To sum up, the stress of bone tissue and displacement were sensitive to the change of implant diameter rather than the change of implant length. These findings indicate that implant diameter has a greater effect on stress distribution of bone tissue than the implant length, and the implants with > 1.5 mm in diameter are suitable for IV osteoid.

Objective To construct short hairpin RNA (shRNA) expression plasmids against the inhibitor of differentiation 2 (Id2) gene and establish a suitable cell model for the role of Id2 in proliferation and differentiation of human Ewing sarcoma cell.Methods Three shRNA sequences targeting Id2 gene were designed and inserted into the pGPU6/GFP/Neo (-shRNA-Id2) expression vectors.The recombinant pGPU6/GFP/Neo-shRNA-Id2 plasmids were introduced into Ewing sarcoma RD-ES cells by liposome-mediated transfection.The knock-down efficiency of Id2 in infected RD-ES cells was verified by Western blot assay.The cell growth and cell cycle changes were evaluated by cell counting and flow cytometry between transfected cells and control cells.Results The Id2 expression decreased 54 ％ and 57 ％,respectively,in RD-ES cell line which were transfected with the shRNA-Id2-543 and shRNA-Id2-593 plasmids compared with the control group cells by Western blot analysis.The cell growth assay demonstrated that the cell number in transfected cells was significantly decreased during 6-7 d compared with the control group (P ＜ 0.05).The cells at the S-phase of cell cycle were increased [(36.60±1.53) ％ and (44.89E2.46) ％ vs (29.73±2.03) ％,P ＜ 0.05],and no significant changes at the G2 phase or even reduction in the transfected cells.Conclusions Id2 stable knock-down cell lines are successfully established.The reduced expression of Id2 is related with decreased cell growth and cell cycle arrest in the S-phase.Id2 maybe plays an important role in proliferation of Ewing sarcoma cell.

Objective To investigate the effect of As_2O_3 on normal mouse bone marrow stromal cells(BMSCs).Methods BMSCs and colony-forming unit-granulocyte macrophage (CFU-GM) were cultured in vitro to observe the quantitative changes of colony-forming unit-fibroblast(CFU-F) and CFU-GM after BMSCs were treated with different concentrations of As_2O_3 respectively;the quantitative changes of CFU-GM supported by BMSCs and granulocyte colony stimulating factor(G-CSF) and IL-11 secreted by BMSCs were also observed after the progenitor cells supported by BMSCs were treated with As_2O_3 for 3,5 and 7 d,respectively.Results The productions of CFU-F and CFU-GM supported by BMSCs were decreased obviously when treated with 7 ?mol?L~(-1) As_2O_3,also the levels of G-CSF and IL-11 secreted by BMSC(P

Objective To evaluate the effect of basic fibroblast growth factor(bFGF) on sheep bone marrow mesenchymal stem cells(BMSCs) proliferation and differentiation in vitro.Methods The sheep BMSCs were isolated and expanded in vitro and divided into experimental group and control group.bFGF(2 ?g?L-1) was added into cell culture medium of experimental group.In control group,no bFGF was added.The morphological differences of BMSCs between two group were observed.Growth curves and alkaline phosphatase(ALP) activity were assessed.In order to observe the influence of bFGF on differentiation potential of BMSCs,osteogenic and adipogenic inductions were conducted on BMSCs in two groups.Results Fibroblast colonies appeared 1 week after seeding of the mononuclear cells.2 weeks later,more colonies formed and each colony became larger.The cell number was 2.5 times of the control when the first passage cells grew to confluence and the ALP activity in experimental group was lower than that in control group(P