BACKGROUND: Previous research has proved that olfactory ensheathing cells can promote neuronal survival and axonal regeneration. OBJECTIVE: To investigate the effect of olfactory ensheathing cell transplantation on the treatment of spinal cord injury of rats. METHODS: A total of 40 healthy adult female SD rats were randomly divided into control and cell transplantation groups, with 20 rats for each group. Ten additional SD rats were used for separation and culture of olfactory ensheathing cells. Spinal cord injury was induced in both control and cell transplantation groups. 2-cm bilateral 8th-10th intercostal nerves were crossly implanted into spinal cord defect region, i.e., proximal white matter and distal gray matter, distal white matter and proximal gray matter. Olfactory ensheathing cells at density of 2×10~6 were locally injected into cell transplantation group, while an equal amount saline was locally injected into control group. Somatosensory evoked potential and motion evoked potential were detected to observe neuro-electrophsiological recovery; BBB was used to evaluate hindlimb motor function; BDA anterograde tracer was used to observe motor conduction recovery. RESULTS AND CONCLUSION: Latency and amplitude of somatosensory evoked potential and motion evoked potential in the cell transplantation group were significantly greater than control group (P < 0.01). BBB scores of cell transplantation group were significantly greater than control group (P < 0.01). BDA-positive nerve fibers in the cell transplantation group were significantly more than control group (P < 0.01). Local injection of olfactory ensheathing cells can improve neuro-electrophysiological changes and promote hindlimb motor functional recovery following spinal cord injury.

BACKGROUND: To improve neural regeneration and functional recovery following spinal cord injury (SCI) remains difficult. Embryonic neural stem cells benefit neuronal survival and promote axonal regeneration.OBJECTIVE: To explore the feasibility of the local injection of embryonic neural stem cells to treat rats with high level SCI, and evaluate its effect by neuroelectrophysiology and motor function of hind limbs.DESIGN, TIME AND SETTING: In vivo experiment of cytology was performed at the Animal Experimental Center of Harbin Medical University from June 2007 to June 2008.MATERIALS: A total of 40 healthy adult SD rats were randomly divided into two groups (n=20): normal saline and cell transplantation groups. In addition, 5 SD rats, pregnant 14 days, were used for preparation of embryonic neural stem cells.METHODS: High level SCI was made in two groups. Briefly, 2 cm of bilateral intercostal nerves respectively from the 8~(th) to 10~(th) costal bones was crossing implanted in the SCI (proximal white matter and distal gray matter; distal white matter and proximal gray matter). Cell transplantation group was locally injected with 2×10~6 embryonic neural stem cells, and normal saline group was injected with the same volume of normal saline.MAIN OUTCOME MEASURES: Somatosensory evoked potential (SEP) and motor evoked potentials (MEP) testing, observation of the recovery of neuroelectrophysiology; BDA anterograde neural tracer; the recovery of motor conduction by the main beam;BBB hind limb motor function score.RESULTS: SEP and MEP latency and amplitude of cell transplantation group were better than normal saline group (P < 0.01).BDA-positive nerve fibers through the tag were found in cell transplantation group, but no in normal saline group. BBB hind limb motor function score of cell transplantation group was significantly improved compared with normal saline group improved (P < 0.01).CONCLUSION: Local injection of embryonic neural stem cells betters neuroelectrophysiology and motor function of hind limbs following high level SCI.

BACKGROUND:Brain injury is a serious central nervous system trauma.However,it is difficult to promote nerve regeneration and functional recovery after brain injury.Sheath cells are conducive to neuronal survival and axonal regeneration.OBJECTIVE:To explore feasibility and effect of olfactory ensheathing cells transplantation on rat brain injury.METHODS:A total of 90 healthy adult male SD rats were selected and 10 were used to prepare olfactory ensheathing cells.The remaining were randomly divided into model control and transplantation groups with 40 animals in each group.Model of middle cerebral artery occlusion was established by thread method.At 1 week,2×10~6 suspension of olfactory sheath cells and an equal volume of sterile saline were injected into two groups,respectively via the carotid artery.Neurological deficits were evaluated by creeping scores;histopathological changes were detected by HE staining,and glial fibrillary acidic protein and neurotrophic factor receptor p75 expression was detected by immunohistochemistry.RESULTS AND CONCLUSION:Compared with the model control group,the neurological deficit scores were significantly reduced in the transplantation group compared with the control group at 1,2,3,and 4 weeks following cerebral ischemia/reperfusion(P ＜ 0.05);the pathological changes in injured brain tissues were ameliorated,the number of nerve cell degeneration and necrosis was significantly reduced,and edema was attenuated.A great amount of glial fibrillary acidic protein and neurotrophic factor receptor p75 expression was detected in the infarct hemisphere following cell transplantation,and little in the contralateral hemisphere and vascular endothelial cells.Negative expression was detected in the model control group.Results show that the olfactory ensheathing cell transplantation is effective on ischemic brain injury in the rats.

Objective To develop a synthetic medical image generation system which can provide test images for the validation of medical image segmentation algorithms.Methods The synthetic image was created based on the deformation of region of interest (ROI) in original clinical images.First the synthetic foreground boundarywas generated by the resampling of the Fourier descriptors of manually segmented foreground boundary in original image.Then all the ROI pixels were divided into 4 categories and their intensities were calculated by texture matching techniques.Results The intracranial hemorrhage image was selected as the original image,and the generated synthetic images were applied to validate the precision and accuracy of multi-threshold segmentation and level set algorithm.Conclusion The proposed system can rapidly generate synthetic images with realistic appearance of clinical cases and well define ground truth foreground boundary.It has strong practicality for quantitative validation of segmentation algorithms.

OBJECTIVE:To analyze the effects of environmental factors (soil,climate,etc.) on the quality of Radix Polygalae.METHODS:The contents of polygalic acid were determined by HPLC.Climate factors including altitude,average annual temperature,average temperature of July,annual precipitation,annual sunshine hours and non-frost days were collected through on-site inspection or from the local meteorological data. Water content,pH and nutritional elements of the soils were determined by routine method,and the reletionship between the content of polygalic acid and the factors were analyzed.RESULTS:There was linear relationship between the content of polygalic acid and climate factors such as average temperature of July,annual precipitation,annual sunshine hours and non-frost days.There was also linear relationship between polygalic acid and soil factors such as total nitrogen,total phosphor,available phosphor,available kalium,organic matters and water content. CONCLUSION:The main climate factors that affect polygalic acid content were annual sunshine hours and non-frost days,while the main soil factors affecting the content of polygalic acid were the content of available phosphor and soil water content.

Objective To investigate the inserting technique of biliary double stents in treating hilar cholangiocarcinoma. Methods 6 patients with hilar cholangiocarcinoma (Bismuth Ⅳ) were treated by percutaneous transhepatic insertion of biliary stents. Double stents were inserted in each patient. Different inserting methods were adopted acording to the branch angles formed by left and right hepatic ducts. Results The jaundice of all patients alleviated or disappeared obviously after stent implantation. The average difference between post-and pre-operation in the serium total bilirubin level was (104?29) ?mol/L(P

The hospital information system(HIS)is processed in digital standardization for information sharing.In this way,all medical information in different hospitals can be compiled and compared,which provides sufficient and reliable information for the fundamental research on public health and medicine.

Objective To evaluate the effects of different positions on cerebral blood flow in patients undergoing laparoscopic surgery.Methods Thirty patients of both sexes,aged 23-64 yr,of American Society of Anesthesiologists physical status Ⅰ or Ⅱ,scheduled for elective laparoscopic surgery,were included.Patients' position was changed using a random number table after induction of anesthesia.Patients were placed in the supine position and tilted 15° head-up or 15° head-down,and the parameters of the middle cerebral artery were monitored using the transcranial Doppler ultrasound.After admission to the operating room (baseline),after induction of anesthesia and before pneumoperitoneum,in the supine position,at head-up tilt and at head-down tilt,the mean blood flow velocity,pulsatility index,resistance index,mean arterial blood pressure (MAP) and heart rate were recorded.Results Compared with the baseline in the supine position,the MAP and bilateral mean blood flow velocity were significantly decreased at head-up tilt,and the MAP and bilateral pulsatility and resistance indices were significantly increased at head-down tilt (P＜0.05).Conclusion During laparoscopic surgery,head-up tilt can lead to a decrease in cerebral blood flow,and head-down tilt exerts no effect on cerebral blood flow.

This study was aimed to explore the effect of heat-nature Chinese herbs of Pepper and Piper longum on hepatic energy metabolism of rats in order to research the impact of ratˊs physical activities with heat-nature Chinese herbs and establish the research foundation of Chinese herbal medicine nature . A total of 30 rats were randomly divided into three groups . Saline was given in the control group . Pepper water extract was given in the Pepper group . And Piper longum water extract was given in the Piper longum group . The administration dosage was 10 ml/kg intragastrically for 30 days . Changes of hepatic energy metabolism related enzymes were determined . The results showed that compared with the control group , Ca2+-ATPase , Ca2+-Mg2+-ATPase , and LDH enzyme of the Pepper group and Piper longum group were significantly increased . And the hepatic glycogen content was significantly reduced . It can obviously increase energy intake , digestible energy and metabolizable energy per body weight in rats . There was no statistical significance between the Pepper group and Piper longum group . It was concluded that the heat-nature Chinese herbs of Pepper and Piper longum can promote the decomposition of hepatic glycogen , increase the activity of LDH enzyme , Ca2+-ATPase , Ca2+-Mg2+-ATPase . It increases the consumption of ATPase in order to increase energy intake , digestible ener-gy and metabolizable energy per body weight in rats and promote the hepatic energy metabolism .

Objective To investigate the inhibitory effects of arsenic trioxide on salivary duct carcinoma cell colony and to explore a new approach to treat salivary duct carcinoma .Methods Human salivary duct carci-noma cancer cells were established .The effects of different concentrations (0.5、1、2、4μmol/L)and time(48,96 H) of arsenic trioxid on salivary duct carcinoma cell colony and the changes of the cell colony were observed with MTT assay and microscopic cell analysis .Results Human salivary duct carcinoma were significantly inhibited by Different concentrations of arsenic trioxide at different times points , and the relationship between the doses and time points are dependent ,cell atypia disappearing apoptosis appears apoptotic bodies .Conclusion Arsenic tri-oxide can significantly inhibit proliferation ,induce differentiation and promote apoptosis in the salivary duct carci-noma cells .