Objective To study the hydrogen sulfide productivity by mononuclear cells in patients with atrial fibrillation (AF). Methods Mononuclear cells were extracted from peripherial blood of 60 AF patients and 30 healthy controls. Modified methylene blue method was used to evaluate the hydrogen sulfide productivity of mononuclear cells. Results Hydrogen sulfide productivity of mononuclear cells from AF patients was 92.5%higher than that from control [10.63 (8.21~14.37) μmol/(min.g protein) vs 5.52 (4.42~8.50) μmol/(min.g protein)]. Hydrogen sulfide productivity of mononuclear cells was correlated with the left atrial transverse diameter (r = 0.276,P < 0.01), while no significant correlation was found between hydrogen sulfide and left ventricular end-diastolic diameter , left atrial anterior posterior diameter , C-reaction protein etc. Conclusion Hydrogen sulfide productivity of mononuclear cells from AF patients is promoted.

AIM:To investigate the expression and function of store-operated calcium channels ( SOCC) in human circulating fibrocytes.METHODS:Peripheral blood mononuclear cells ( PBMCs) were isolated and cultured in ser-um-free media.After 7 d, the PBMCs differentiated into fibrocytes.RT-PCR and real-time PCR were performed to deter-mine the mRNA expression of ORAI1-3 and STIM1-2 in the fibrocytes.SOCC inhibitor SKF-96365 was used to elucidate the role of SOCC in the differentiation of fibrocytes.RESULTS:The results of real-time PCR showed that the mRNA ex-pression of ORAI1-3 and STIM1-2 was positive in cultured fibrocytes.SKF-96365 (10μmol/L) significantly inhibited the differentiation of fibrocytes.CONCLUSION:SOCC-related proteins ORAI1-3 and STIM1-2 are abundantly expressed in the fibrocytes, and may play an important role in the differentiation of these cells.

We implemented tutorial system by relying on young teachers in hospitals and taking undergraduates in Capital Medical University as the main body aiming at cultivating students' clinical and scientific research abilities.Practice has proved that tutor system method is of great value to cultivate students' comprehensive clinical ability,scientific research ability and innovation ability.Meanwhile,this method is effective in improving clinical teaching effect.

Objective:To study the association between high-normal thyroid-stimulating hormone(TSH) and new onset postoperative atrial fibrillation(POAF) after off-pump coronary artery bypass graft(OPCABG).Methods:Between July 2017 and January 2019, a total of 219 patients who underwent OPCABG in Beijing Chaoyang Hospital were retrospectively analyzed. The patients were divided into two groups, POAF group(64 cases) and non-POAF group(155 cases), in accordance with the occurrence of POAF. POAF was compared across groups with TSH quartile groups with euthyroidism. The perioperative clinical parameters of the two groups were analyzed by univariate analysis. Then, statistically significant factors in the univariate analysis were subjected to multivariate logistic regression analysis to determine if it was an independent risk factor for POAF. Results:Compared with TSH Q4 and TSH Q1- Q3, the incidence of POAF was statistically different( P=0.008). Logistic regression analysis showed that age≥60 years( OR=2.672, P=0.010), HCY≥18 μmol/L( OR=1.956, P=0.039), and high-normal thyroid-stimulating hormone( OR=2.856, P=0.036) were the independent risk factors of POAF after OPCABG. Conclusion:High-normal thyroid-stimulating hormone is the independent risk factor of POAF after OPCABG.