Objective To observe the pathological changes of major organs in rats with acute Dysosma versipellis poisoning and investigate the toxic mechanismand the injuries of target tissues and organs. Methods Forty Sprague-Daw ley (SD) rats were random ly divided into three experimental groups, which were given the gavage with 0.5, 1.0 and 2.0LD50 doses of Dysosma versipellis decoction, and one con-trol group, which was given the gavage with 1.0LD50 dose of normal saline. The rats were sacrificed 14 days after Dysosma versipellis poisoning and sam ples including brain, heart, liver, lung, and kidney were taken. After pathological process, the pathological changes of the major organs and tissues were observed by light microscope and electron microscope. The experimental data were statistical analyzed by x2 test. Results The observations of light microscopy: loose cytoplasmof neurons with loss of most Nissl bodies;swelling of m yocardial cells with disappearance of intercalated disk and striations;hepato-cellular edema with ballooning degeneration; and swelling epithelial cells of renal proximal convoluted tubule with red light coloring protein-like substances in the tube. The observations of electron microscopy:the structures of cell mem brane and nuclear mem brane of neurons were destroyed;cytoplasmof neurons, obvious edema;and most organelles, destroyed and disappeared. The mortalities of rats after acute poi-soning of the four groups increased with doses (P<0.05). Conclusion Acute Dysosma versipellis poisoning can cause multi-organ pathological changes. There is apositive correlation between the toxic effect and the dosage. The target tissues and organs are brain (neurons), heart, liver and kidney.

Objective To investigate effect of salidroside on the function and activation of rheumatoid arthritis fi-broblast-like synoviocyte (HFLS-RA) by regulating the miR-20 a-5 p/tissue inhibitor of metalloproteinase-2 (TIMP2) axis.Methods HFLS-RA cells were used as the research object.HFLS-RA cells were separated into control group, tumor necrosis factor-α(TNF-α) group, salidroside group, inhibitor NC group, miR-20a-5p inhibi-tor group, salidroside+mimic NC group, and salidroside+miR-20a-5p mimic group.qRT-PCR was applied to de-tect the expression of miR-20a-5p in HFLS-RA cells;enzyme-linked immunosorbent assay(ELISA) was applied to detect the levels of interleukin-1β (IL-1β) and IL-6 in the supernatant of HFLS-RA cells; cell counting kit-8 (CCK-8) method and 5-ethynyl-2 '-deoxyuridine (EdU) staining were applied to detect HFLS-RA cell prolifera-tion;scratch experiment was applied to detect HFLS-RA cell migration;Western blot was applied to detect the ex-pression of TIMP2, CyclinD1, and matrix metalloproteinase (MMP)-9 proteins in HFLS-RA cells;double lucifer-ase was applied to verify the relationship between miR-20a-5p and TIMP2.Results Compared with the control group, the expression of miR-20a-5p, the levels of IL-1β and IL-6, OD450 value, EdU positive cell rate, scratch healing rate, and the expression of CyclinD1 and MMP-9 proteins in the TNF-α group increased, the expression of TIMP2 protein decreased (P<0.05);compared with the TNF-α group, the expression of miR-20a-5p, the levels of IL-1β and IL-6, OD450 value, EdU positive cell rate, scratch healing rate, and CyclinD1 and MMP-9 proteins expression decreased, the expression of TIMP2 protein increased in salidroside group (P<0.05); compared with the TNF-α group and inhibitor NC group, the expression of miR-20a-5p, the levels of IL-1β and IL-6, OD450 val-ue, EdU positive cell rate, scratch healing rate, and the expression of CyclinD1 and MMP-9 proteins in the miR-20a-5p inhibitor group decreased, the expression of TIMP2 protein increased (P<0.05);compared with the sali-droside group and the salidroside+mimic NC group, the expression of miR-20a-5p, the levels of IL-1β and IL-6, OD450 value, EdU positive cell rate, scratch healing rate, and the expression of CyclinD1 and MMP-9 proteins in the salidroside+miR-20a-5p mimic group increased, the expression of TIMP2 protein decreased (P <0.05).There was a targeted regulatory relationship between miR-20a-5p and TIMP2.Conclusion Salidroside may inhibit TNF-α-induced HFLS-RA cell proliferation, migration and inflammatory response by regulating miR-20a-5p/TIMP2 .

Objective:To investigate the clinical value of Rotarex ? S in debulking the femoropopliteal calcified lesion. Methods:The data of 5 patients with femoropopliteal calcified lesions treated with Rotarex ? S combined with drug balloon treatment admitted to the First Affiliated Hospital of Dalian Medical University from March 2020 to December 2021 were retrospectively analyzed. The success rate and complications of all operations were recorded. CT software was used to compare the effective lumen area of the target lesion area before and after surgery in all cases, and the ischemic grade of the affected limb before and after surgery was recorded in all patients. Results:All 5 femoropopliteal calcified lesions were successfully treated with Rotarex ? S debulking followed with drug-coated balloon angioplasty without flow-limited dissections, vascular perforation and distal embolizations, with a success rate of 100%. The effective lumen area for calcified lesions were increased 9-15.11 mm 2 (median: 13 mm 2). The Rutherford classifications were improved from R 2-4 to R 1-2. Conclusions:Femoropopliteal calcified lesion debulking with Rotarex ? S was safe and effective.

Objective: To investigate the changes of microorganisms and metabolites in joint effusion of patients with Rheumatoid arthritis(RA), Osteoarthritis(OA) and Urarthritis(UA). To provide new ideas for the study of the effect of microbiota on the pathogenesis of arthritis. Methods: Joint effusion samples were collected from 20 patients with RA, 20 patients with OA, and 20 patients with UA. 16S rRNA gene sequencing and untargeted ultra-high performance Liquid chromatography-mass spectrometry(LC-MS) were used to explore the differences in microorganisms and metabolites among the three groups. Pearson correlation analysis was used to detect the correlation between effusion microbiota and metabolites. Results: There were differences in microbial diversity and microbiota composition among the three groups. Combined with VIP>1 from OPLS-DA andP<0.05 from two-tailed Students t-test, 45 differential metabolites(Between RA and OA groups), 38 differential metabolites(Between UA and OA groups) and 16 differential metabolites(Between RA and UA groups), were identified. GO analysis and KEGG pathway analysis showed that the differential metabolic pathways among the three groups were mainly concentrated in citric acid cycle(TCA cycle), nucleotide metabolism, amino acid metabolism and glycolysis pathway. Correlation analysis of joint effusion microbiota and metabolites suggested that bacteria enriched in the three groups of joint effusion, such asPrevotella,Clostridium ruminosus,Prevotellaceae＿UCG-001, were related to many key metabolites such as lysozyme, uric acid, glucose, and L-glutamine. Conclusion This study shows that there are a variety of bacterial flora in joint cavity effusion of RA, OA, and UA patients, and the differential metabolites produced by them are involved in the pathogenesis of the three types of arthritis by affecting a variety of metabolic pathways.