1.Risk factors related to fetal death in patients with severe preeclampsia
Guangyuan LIAO ; Yan ZHOU ; Yuanmei GAO ; Jing LI ; Zhong XU
The Journal of Practical Medicine 2017;33(22):3726-3728
Objective To investigate the risk factors of fetal death in patients with severe preeclampsia Methods Clinical data of 70 cases with severe preeclampsia were analyzed retrospectively,including 53 cases of fetal survival and 17 cases of death.Results There was shorter pregnancy duration in fetal death group (P < 0.05) but there were no significant differences in the age,serum albumin level,systolic blood pressure,diastolic blood pressure and mean arterial pressure in 2 groups (P > 0.05).Pulmonary edema was correlated to gestational week (P < 0.05);the higher the gestational age was,the higher the occurrence of pulmonary edema.Conclusion Earlier onset of severe preeclampsia predicts higher rate of fetal death.
2.Blocking ERK signaling pathway lowers MMP-9 expression to alleviate brain edema after traumatic brain injury in rats.
Zhaohua TANG ; Wentao WANG ; Zili LIU ; Xiaochuan SUN ; Zhengbu LIAO ; Feilan CHEN ; Guangyuan JIANG ; Gang HUO
Journal of Zhejiang University. Medical sciences 2020;40(7):1018-1022
OBJECTIVE:
To investigate the effects of blocking the activation of ERK pathway on the expression of matrix metalloproteinase-9 (MMP-9) and the formation of cerebral edema in SD rats after brain injury.
METHODS:
Ninety SD rats were randomly divided into 3 equal groups, including a sham-operated group, modified Feeney's traumatic brain injury model group, and ERK inhibition group where the ERK inhibitor SCH772984 (500 μg/kg) was injected via the femoral vein 15 min before brain trauma. At 2 h and 2 days after brain trauma, the permeability of blood-brain barrier was assessed by Evans blue method, the water content of the brain tissue was determined, and the phosphorylation level of ERK and the expression level of MMP-9 mRNA and protein were measured by RT-PCR and Western blotting.
RESULTS:
Compared with the sham-operated group, the rats with brain trauma exhibited significantly increased level of ERK phosphorylation at 2 h and significantly increased expression of MMP-9 mRNA and protein 2 days after the injury ( < 0.01). Treatment with the ERK inhibitor significantly decreased the phosphorylation level of ERK after the injury ( < 0.01), suppressed over-expression of MMP-9 mRNA and protein 2 days after the injury ( < 0.01). The permeability of blood-brain barrier increased significantly 2 h after brain trauma ( < 0.05) and increased further at 2 days ( < 0.01); the water content of the brain did not change significantly at 2 h ( > 0.05) but increased significantly 2 d after the injury ( < 0.01). Treatment with the ERK inhibitor significantly lowered the permeability of blood-brain barrier and brain water content after brain trauma ( < 0.01).
CONCLUSIONS
Blocking the activation of ERK pathway significantly reduced the over-expression of MMP-9 and alleviates the damage of blood-brain barrier and traumatic brain edema, suggesting that ERK signaling pathway plays an important role in traumatic brain edema by regulating the expression of MMP-9.
Animals
;
Brain Edema
;
drug therapy
;
etiology
;
Brain Injuries, Traumatic
;
complications
;
drug therapy
;
Gene Expression Regulation, Enzymologic
;
drug effects
;
Indazoles
;
pharmacology
;
therapeutic use
;
MAP Kinase Signaling System
;
drug effects
;
Matrix Metalloproteinase 9
;
genetics
;
Piperazines
;
pharmacology
;
therapeutic use
;
Protein Kinase Inhibitors
;
pharmacology
;
therapeutic use
;
Random Allocation
;
Rats
;
Rats, Sprague-Dawley
3.Blocking ERK signaling pathway lowers MMP-9 expression to alleviate brain edema after traumatic brain injury in rats.
Zhaohua TANG ; Wentao WANG ; Zili LIU ; Xiaochuan SUN ; Zhengbu LIAO ; Feilan CHEN ; Guangyuan JIANG ; Gang HUO
Journal of Southern Medical University 2020;40(7):1018-1022
OBJECTIVE:
To investigate the effects of blocking the activation of ERK pathway on the expression of matrix metalloproteinase-9 (MMP-9) and the formation of cerebral edema in SD rats after brain injury.
METHODS:
Ninety SD rats were randomly divided into 3 equal groups, including a sham-operated group, modified Feeney's traumatic brain injury model group, and ERK inhibition group where the ERK inhibitor SCH772984 (500 μg/kg) was injected via the femoral vein 15 min before brain trauma. At 2 h and 2 days after brain trauma, the permeability of blood-brain barrier was assessed by Evans blue method, the water content of the brain tissue was determined, and the phosphorylation level of ERK and the expression level of MMP-9 mRNA and protein were measured by RT-PCR and Western blotting.
RESULTS:
Compared with the sham-operated group, the rats with brain trauma exhibited significantly increased level of ERK phosphorylation at 2 h and significantly increased expression of MMP-9 mRNA and protein 2 days after the injury ( < 0.01). Treatment with the ERK inhibitor significantly decreased the phosphorylation level of ERK after the injury ( < 0.01), suppressed over-expression of MMP-9 mRNA and protein 2 days after the injury ( < 0.01). The permeability of blood-brain barrier increased significantly 2 h after brain trauma ( < 0.05) and increased further at 2 days ( < 0.01); the water content of the brain did not change significantly at 2 h ( > 0.05) but increased significantly 2 d after the injury ( < 0.01). Treatment with the ERK inhibitor significantly lowered the permeability of blood-brain barrier and brain water content after brain trauma ( < 0.01).
CONCLUSIONS
Blocking the activation of ERK pathway significantly reduced the over-expression of MMP-9 and alleviates the damage of blood-brain barrier and traumatic brain edema, suggesting that ERK signaling pathway plays an important role in traumatic brain edema by regulating the expression of MMP-9.
Animals
;
Blood-Brain Barrier
;
Brain Edema
;
Brain Injuries, Traumatic
;
MAP Kinase Signaling System
;
Matrix Metalloproteinase 9
;
Rats
;
Rats, Sprague-Dawley