The paper concluded systematically the progress of study on KCNQ1 in recent years.Firstly,the structure of ? subunit or ? subunit and its interaction between each other were explained in the paper.Secondly,the physiological function of complex KCNQ1/KCNE was interviewed,such as many kinds of inherited cardiac arrhythmias induced by mutation of KCNQ1 or KCNE and secretion of KCNQ1 in different epithelial cells.Eventually,the paper introduced the study of antagonists and agonists on KCNQ1 channel.

Objective To analyze the expression and the role of Toll-like receptor 4 (TLR4) and the effect of N-acetylcysteine (NAC) on hepatic ischemia- reperfusion (I/R) injury.Methods The rat model of 45 min hepatic ischemia followed by 24 h reperfusion was used. Animals were divided into 3 groups: the pseudoperated control group (P), I/R group and treated group with NAC at a dose of 300 mg/kg at 5 min before reperfusion (I/R-NAC). Plasma concentrations of ALT, AST and lipopolysaccharide (LPS) at various time points were measured. The expression of TLR4 protein and mRNA was detected in liver tissue by using immunohistochemistry and RT-PCR.Results The TLR4 expression, plasma ALT, AST and LPS levels were markedly increased during 45 min hepatic ischemia and 24 h reperfusion, but they were lower in I/R-NAC group and no alteration almost in group P. The plasma concentrations showed a significant correlation between ALT, AST and LPS at different time points in I/R group and I/R-NAC group.Conclusion LPS and TLR4 play a prominent role in the pathophysiological processes of I/R and NAC can be of therapeutic potential for reducing the expression of TLR4 mRNA and alleviating endotoxemia derived from gut.

Objective: To investigate the effect of diabetes on the intensity of sarcoplasmic reticulum Ca2+-ATPase (SERCA2a)-SUMOylation and SERCA2a activity of myocardium in experimental rats.
Methods: The 8 weeks old SD rats were divided into 2 groups, Diabetic group, with diet-induced type 2 diabetic rats and Control group, with normal rats. The systolic and diastolic cardiac functions were evaluated by echocardiography and left ventricular pressure measurement. The intensity of SERCA2a-SUMOylation was examined by co-immunoprecipitation and SUMOylation kit.
Results: Compared with Control group, Diabetic group had decreased systolic and diastolic cardiac functions, especially for diastolic function;decreased SERCA2a protein expression and intensity of SUMOylation;decreased SUMOylation E2 (Ubc9 ) protein expression. The protein levels of SUMO1, SAE1 and SAE2 were similar between 2 groups.
Conclusion: The intensity of SERCA2a-SUMOylation and Ubc9 decreased in diabetic myocardium which implies that SERCA2a-SUMOylation and Ubc9 were closely related to the damage of diabetic myocardium in experimental rats.

Objective To investigate the epidemiology of respiratory viruses in children from Wuxi area.Methods A total of 2 747 cases of children diagnosed with acute respiratory infection in Wuxi during 2011 —2014 were collected.Reverse transcription-polymerase chain reaction was used to detect nine kinds of respiratory viruses,including influenza virus A (Flu A),influenza virus B (Flu B),parainfluenza virus (PIV)Ⅰ-Ⅳ,adenovirus (ADV),respiratory sycytial virus (RSV),human metaneumovirus (hMPV), human bocavirus (HBov),human coronaviruses (hCov)and human rhinovirus (HRV).The categorical data were compared using chi square test.Results A total of 856 among the 2 747 samples were tested positive for respiratory virus nucleic acid,with the positive rate of 31 .16%.The viral distribution was uneven in different seasons,and the infection peaked in winter and spring.The virus detection rate was highest in age 1 to 2 year group (up to 40.18%),and followed by age 6 to 12 year group (32.63%).Flu A virus was the most frequently detected virus,accounting for 7.54% (207/2 747);followed by PIV, accounting for 6.95 % (191/2 747);and Flu B accounted for 4.22%(116/2 747).There were 84 cases of mixed infection of two or more kinds of respiratory viruses,with positive rate of 3.06% (84/2 747 ). Conclusions Our study suggests that Flu A is the most common pathogen in children with acute respiratory infections in Wuxi area during 2011 —2014;virus detection rate is highest in age 1 to 2 year group;and parainfluenza virus is almost detected throughout the year,while the rest of respiratory viruses are commonly seen in winter and spring.

OBJECTIVE: To evaluate the safety and efficacy of Yiqi Pingchuan Granule in treating acute attack of asthma due to qi deficiency and cold syndrome. METHODS: A randomized controlled trial was conducted. A total of 80 patients with an acute attack of asthma were included. They were allocated into two groups randomly in a ratio of three to one. Sixty patients in the treatment group received Yiqi Pingchuan Granule and 20 patients in the control group received Ruyi Dingchuan Pill. Patients in both groups were treated for 7 days. RESULTS: There were no significant differences in traditional Chinese medicine syndrome, clinical symptoms and lung function between the two groups (P>0.05). After treatment, the forced expiratory volume in 1 second was increased in the treatment group (P<0.05), and the peak expiratory flow was accelerated in the control group (P<0.05). No significant side effects were noted in both groups. CONCLUSION: Yiqi Pingchuan Granule is safe and effective in treating acute attack of asthma due to qi deficiency and cold syndrome.

Objective: To investigate the epidemiological characteristics and causes of an outbreak of respiratory infection in schools. Methods: Epidemiological investigation was carried out on respiratory infection cases in schools. Pharyngeal swab samples of children were collected for respiratory tract virus nucleic acid detection, and hexon genes were amplified. Positive PCR result were sequenced. Results: All cases were in school, involving 2 classes, 12 cases in class 2 (3) and 13 cases in class 3 (4), all on the west side of the 2nd and 3rd floors of teaching building 2. From September 3 to September 12, a total of 25 cases were involved, with the onset peak on the 10th and 11th. The clinical features were fever, cough, sore throat and aching limbs, etc. The onset was aggregated, the course of the disease was about 5 d, the prognosis was good, and there were no deaths. There are 13 males and 12 females. The males to females ratio is 1.08∶1. The age distribution is 7-9 years, with an average of 8.6 years. The result of fluorescence quantitative PCR were negative for influenza A/B virus and positive for adenovirus. The products of positive samples after PCR amplification were sequenced as AdV-3 and AdV-4. Conclusions The combined result of epidemiological investigation and laboratory tests demonstrated that this epidemic of school respiratory tract infection is an upper respiratory tract infection caused by adenovirus B3 and E4 mixed infection.

Objective:To evaluate the immunogenicity of recombinant factor H binding protein(fHBP) by detecting serum antibody titer and serum bactericidal antibody test (SBA).Methods:fHBP sequence was selected and synthesized, connected to plasmid pET43.1a, transformed to Escherichia coli BL21(DE3), and expressed two recombinant fHBP proteins, included two subfamilies, fHBPA and fHBPB. After purification, the recombinant fHBP proteins were immunized to rabbits and mice. The immune antiserum titer and the bactericidal titer to epidemic strains of meningococcal bacteria group B were measured by ELISA and SBA respectively. Results:The antiserum titer of fHBP immunized rabbits was greater than 2.0×10 6, and that of immunized mice was not less than 1.0×10 6. fHBP immunized rabbit serum had bactericidal titer more than 1∶128 to 41 strains A subfamily and 20 strains B subfamily in the SBA against 69 endemic strains, and there was no cross-protection between the subfamily bacteria. The bactericidal titers of mouse serum immunized fHBPA to strains A subfamily such as Nm210902 Nm211009、Nm450522 were 1∶1 024, 1∶608、1∶861, to Nm510703、Nm311304、Nm431002 were 1∶234、1∶861、1∶430 respectively, and mouse serum immunized fHBP B to strains B subfamily Nm311302、Nm311304、Nm431002 were 1∶876、1∶274、1∶1858, all of three strains were positive in bactericidal titers. Conclusions:the titer of fHBP antiserum was higher than 1.0×10 6, the bactericidal titer was no less than 1∶128 to 61 epidemic strains, and it has a 94.2% protective effect on 69 meningococcal epidemic strains group B.

Objective To investigate the risk factors of fetal death in patients with severe preeclampsia Methods Clinical data of 70 cases with severe preeclampsia were analyzed retrospectively,including 53 cases of fetal survival and 17 cases of death.Results There was shorter pregnancy duration in fetal death group (P ＜ 0.05) but there were no significant differences in the age,serum albumin level,systolic blood pressure,diastolic blood pressure and mean arterial pressure in 2 groups (P ＞ 0.05).Pulmonary edema was correlated to gestational week (P ＜ 0.05);the higher the gestational age was,the higher the occurrence of pulmonary edema.Conclusion Earlier onset of severe preeclampsia predicts higher rate of fetal death.

Objective To study the epidemiological characteristics and type of human adenovirus (HAdv) in Wuxi area in 2011-2013.Methods A total of 1983 acute respiratory infections samples were collected from Wuxi People's Hospital during 2011-2013.HAdv was detected by Real-time PCR,the positive samples were inoculated into Hep-2 cells to isolate the viruses.The amplificons were purified and sequenced,and the sequences were analyzed by Bioedit and MEGA 5.0.Results Human adenovirus was appeared throughout the year in Wuxi area during 2011-2013.In winter and spring,the highest rate (44.26％) was detected in 1-3 years old children.There were 4 strains (13.33％) for HAdV-3,6 strains for HAdV-7(20.00％) of B subgroup,5 strains (16.66％) for HAdV-1,8 strains (26.67％) for HAdV-2,3 strains (10.00％) for HAdV-5,4 strains (13.33％) for HAdV-6 of C subgroup.The phylogenetic tree formed two independent branches and C subgroup gradually became advantage.Conclusions Human adenovirus had obvious seasonal characteristics in Wuxi area during 2011-2013,mainly in winter and spring;1-3 years old children were the main susceptible population.Six types of B and C subgroups were the dominant epidemic strains,with the epidemic tendency transformed from subgroup B to C.

Objective To investigate genetic changes in hemagglutinin (HA) and neuraminidase (NA) protein sequences of novel H7N9 during the first and second outbreak in Wuxi.Methods RNA was extracted from patients and environment samples for detecting target gene.Gene sequencing of HA and NA was performed for the PCR amplicons.Phylogenetic and molecular characterizations were analyzed by Bioedit and MEGA software.Results Gene comparison showed that the similarity in the nucleic acids of HA found in the two outbreaks ranged from 99.09％ to 99.55％,amino acid similarity of 99.09％ to 99.55％,except for the substitution of Ell6G,Q154R,L213S,C421Y,M475I in the second outbreak.Meantime,the amino acid similarity of NA was 99.09％-99.77％.The phylogenetic tree indicated that environment strain appeared in both of the two clusters.Conclusions Phylogenetic and molecular characterizations suggest that the infection of novel H7N9 avian influenza virus has not been prevalent among public in Wuxi.