1.Current research status and progress of stem cells therapy for degenerative intervertebral disc regeneration.
Guangyou XIE ; Furong LU ; Haitao YANG
Journal of Biomedical Engineering 2014;31(6):1419-1422
Low back pain caused by intervertebral disc degeneration is a common clinical chronic disease. The regenerative ability of intervertebral disc tissue is extremely poor. Meanwhile, current treating methods can not fundamentally solve such problems. With the increasing awareness of the mechanism of disc degeneration and the rapid development of the fields of cellular and molecular biology, gene and materials engineering, using stem cells and tissue engineering technology to slow down or reverse the progress of disc degeneration may become possible. The author reviewed the application of stem cells for treating degenerative discs from present researching status and concepts for the future in the combination of researches reported both at home and abroad.
Humans
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Intervertebral Disc
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pathology
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Intervertebral Disc Degeneration
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therapy
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Low Back Pain
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therapy
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Regeneration
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Stem Cell Transplantation
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Tissue Engineering
2.Genetic Variation of Taenia Pisiformis Collected from Sichuan, China, Based on the Mitochondrial Cytochrome b gene.
Deying YANG ; Yongjun REN ; Yan FU ; Yue XIE ; Huaming NIE ; Xiang NONG ; Xiaobin GU ; Shuxian WANG ; Xuerong PENG ; Guangyou YANG
The Korean Journal of Parasitology 2013;51(4):449-452
Taenia pisiformis is one of the most important parasites of canines and rabbits. T. pisiformis cysticercus (the larval stage) causes severe damage to rabbit breeding, which results in huge economic losses. In this study, the genetic variation of T. pisiformis was determined in Sichuan Province, China. Fragments of the mitochondrial cytochrome b (cytb) (922 bp) gene were amplified in 53 isolates from 8 regions of T. pisiformis. Overall, 12 haplotypes were found in these 53 cytb sequences. Molecular genetic variations showed 98.4% genetic variation derived from intra-region. F(ST) and Nm values suggested that 53 isolates were not genetically differentiated and had low levels of genetic diversity. Neutrality indices of the cytb sequences showed the evolution of T. pisiformis followed a neutral mode. Phylogenetic analysis revealed no correlation between phylogeny and geographic distribution. These findings indicate that 53 isolates of T. pisiformis keep a low genetic variation, which provide useful knowledge for monitoring changes in parasite populations for future control strategies.
Animals
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China
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Cytochromes b/*genetics
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*Genetic Variation
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Helminth Proteins/*genetics
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Humans
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Mitochondria/*genetics
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Molecular Sequence Data
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Phylogeny
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Rabbits
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Taenia/classification/genetics/*isolation & purification
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Taeniasis/*parasitology
3.Expression of Translationally Controlled Tumor Protein (TCTP) Gene of Dirofilaria immitis Guided by Transcriptomic Screening.
Yan FU ; Jingchao LAN ; Xuhang WU ; Deying YANG ; Zhihe ZHANG ; Huaming NIE ; Rong HOU ; Runhui ZHANG ; Wanpeng ZHENG ; Yue XIE ; Ning YAN ; Zhi YANG ; Chengdong WANG ; Li LUO ; Li LIU ; Xiaobin GU ; Shuxian WANG ; Xuerong PENG ; Guangyou YANG
The Korean Journal of Parasitology 2014;52(1):21-26
Dirofilaria immitis (heartworm) infections affect domestic dogs, cats, and various wild mammals with increasing incidence in temperate and tropical areas. More sensitive antibody detection methodologies are required to diagnose asymptomatic dirofilariasis with low worm burdens. Applying current transcriptomic technologies would be useful to discover potential diagnostic markers for D. immitis infection. A filarial homologue of the mammalian translationally controlled tumor protein (TCTP) was initially identified by screening the assembled transcriptome of D. immitis (DiTCTP). A BLAST analysis suggested that the DiTCTP gene shared the highest similarity with TCTP from Loa loa at protein level (97%). A histidine-tagged recombinant DiTCTP protein (rDiTCTP) of 40 kDa expressed in Escherichia coli BL21 (DE3) showed immunoreactivity with serum from a dog experimentally infected with heartworms. Localization studies illustrated the ubiquitous presence of rDiTCTP protein in the lateral hypodermal chords, dorsal hypodermal chord, muscle, intestine, and uterus in female adult worms. Further studies on D. immitis-derived TCTP are warranted to assess whether this filarial protein could be used for a diagnostic purpose.
Animal Structures/chemistry
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Animals
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Antibodies, Helminth/blood
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Antigens, Helminth/chemistry/*genetics/immunology/*isolation & purification
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Cloning, Molecular
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Dirofilaria immitis/chemistry/*genetics/immunology
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Disease Models, Animal
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Dogs
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Escherichia coli/genetics
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Gene Expression
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Molecular Sequence Data
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Molecular Weight
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Recombinant Fusion Proteins/chemistry/genetics/immunology/isolation & purification
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Sequence Analysis, DNA
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Tumor Markers, Biological/chemistry/*genetics/immunology/*isolation & purification