Objective To investigate the clinical characteristics,diagnosis and treatment of primary gastroin- testinal malignant lymphoma(PGML).Methods Twenty-two PGML patients were collected from January 1999 to December 2004,and their clinical characteristics,diagnosis,treatment and prognosis were analyzed,Results Among the 22 cases,PGMI commonly attacked stomach,ileocecal region and colon respectively,and the main clinical mani- festations were abdominal pain and bloody stools.50 % of the patients were confirmed by endoscopic biopsy.The pa~ tients treated by operation and chemotherapy turned to better to different extents.Conclusion PGML lacks specific manifestations,thus it has higher rate of misdiagnosis.There are good responses to the assistant chemotherapy and radiotherapy.

Object To study the suitable conditions for the pac king of volatile oil in Rukang Capsule, which will inhibit the loss of volatile oil. Methods With the packing rate as index, even design method wa s applied to investigate the optimal packing conditions. Results The optimal packing conditions were found to be: volatile oil volume (mL)-cy c lodextrin (g)=1∶6, the packing process should be carried out for 120 min under 40 ℃. Conclusion The repetition rate is very good in the repeti tion experiments.

AIM:To study the effect of human complement C 5b～9 complex on nitric oxide(NO) synthesis of glomerular mesangial cells (MC). METHODS: First, the human complement C 5b～9 complexes were isolated and glomerular MC of rats were cultured. Second, the MC were stimulated with C 5b～9 complex and changes of metabolism products of NO(NO 3 and NO 2) in MC culture supernatant at 6,24 and 48 hours after C 5b～9 stimulating were detected. Moreover, cGMP levels in cultured MC were also measured. RESULTS: NO 3/NO 2 contents from culture supernatant and cGMP levels in MC were increased parallelly after C 5b～9 complex stimulation. Further, NO synthesis was inhibited by L-NG-nitro-arginine-methylester(L-NAME). CONCLUSION: NO synthesis of rat glomerular MC was incerased by human complement C 5b～9 stimulation.

AIM:To investigate the synergistic anti-proliferation effect of aspirin and 5-fluorouracil on the co-lon cancer cells and its mechanism .METHODS: Colon cancer cells were divided into 4 groups: control group , aspirin group, 5-fluorouracil group and aspirin +5-fluorouracil group .Synergistic anti-proliferation effect of aspirin and 5-fluoroura-cil on the colon cancer cells was observed by MTT assay .Apoptosis-inducing effect and mechanism were detected by Hoechst 33258 staining, caspase activity assay and flow cytometry analysis .The mRNA and protein levels of apoptosis-re-lated proteins were evaluated by real-time PCR and Western blotting .RESULTS:5-Fluorouracil inhibited proliferation of HCT116 and SW620 colon cancer cells effectively , and low concentration of aspirin exerted synergistic inhibitory effect .5-Fluorouracil induced apoptotic morphology and increased caspase activity and sub -G1 phase in HCT116 cells.The synergis-tic effect of aspirin obviously enhanced apoptotic ratio and caspase activity .Moreover , 5-fluorouracil inhibited the mRNA and protein expression of Bcl-2, which was amplified by low concentration of aspirin .CONCLUSION:Aspirin and 5-flu-orouracil had a synergistic anti-proliferation effect on the colon cancer cells through apoptosis pathway .

Six compounds were isolated from the dried leaves and tender branches of Rabdosia ma-crocalyx (Dunn ) Hara. Their structures were identified as excisanin A, excisanin B, rabdol-oxin B, ursolic acid, ?-sitosterol and palmitic acid.

In recent years, immune checkpoint inhibitors are a milestone in the treatment of lung cancer. There are many kinds of immune checkpoints, which are closely related to the efficacy and drug resis-tance of immunotherapy, including programmed death-1 (PD-1), programmed death ligand-1 (PD-L1), cytotoxic T-lymphocyte antigen 4 (CTLA-4), killer cell immunoglobulin-like receptor (KIR), T cell immunoglobulin and ITIM domain (TIGIT), T-cell immunoglobulin and mucin domain-containing protein 3 (TIM-3), etc. PD-1/PD-L1 inhibitors have been approved by China National Medical Products Administration and U. S. Food and Drug Administration for the first-line treatment of lung cancer, which can improve overall survival and progression-free survival of patients. The double immunotherapies of CTLA-4 inhibitors or TIGIT inhibitors combined with PD-1/PD-L1 inhibitors also achieve good results, however, more serious adverse events may occur.The KIR and TIM-3 targets are closely related to the drug resistance of immunotherapy.

Objective To observe the behavior and the changes of neurologic and his-topathologic structure of rats injected lidocaine-bupivacaine mixtures in different ratio.Methods Af-ter successful intrathecal catherization and positive lidocaine test,48 rats were randomly allocated into 6 groups with 8 rats in each group.Rats received intrathecally NS (group S),mixture of 2.5% lido-caine and 1.875% bupivacaine (group LB13),3.33%lidocaine and 1.67% bupivacaine (group LB12),5% lidocaine and 1.25% bupivacaine (group LB11),6.67% lidocaine and 0.83% bupiv-acaine (group LB21),or 7.5% lidocaine and 0.625% bupivacaine (group LB31).TFL and PWT on consecutive 4 days,recovery time of lower limbs and the pathologic changes in the spinal cord by method of microscopy and electron scope were observed by a blind investigator.Results TFL in group LB31was significantly longer(P <0.05)and hind legs motor function and recover time pro-longed obviously in group LB31;Edema vacuolar degeneration and interstitial edema in white matter of posterior horn of spinal cord were found in group LB31.The histopathologic score was obviously higher in group LB31 compared with other groups (P <0.05).On transmission electroscope,differ-ent levels of ultrastructural changes were found in all groups except in group S .Typical changes included interstitial edema ,loosening of the laminae of myelinated fibers , axon swelling and local degeneration with most serious injury in group LB31.Conclusion The spi-nal injury following intrathecal local anesthetics mixtures was more serious with higher concentration of lidocaine in the mixture.Lidocaine concentration lower than 5% mixed with 1.25% bupivacaine intrathecally induces no obvious spinal neurotoxicity.

Objective To observe the behavior and the changes of neurologic and his-topathologic structure of rats injected lidocaine-bupivacaine mixtures in different ratio.Methods Af-ter successful intrathecal catherization and positive lidocaine test,48 rats were randomly allocated into 6 groups with 8 rats in each group.Rats received intrathecally NS (group S),mixture of 2.5% lido-caine and 1.875% bupivacaine (group LB13),3.33%lidocaine and 1.67% bupivacaine (group LB12),5% lidocaine and 1.25% bupivacaine (group LB11),6.67% lidocaine and 0.83% bupiv-acaine (group LB21),or 7.5% lidocaine and 0.625% bupivacaine (group LB31).TFL and PWT on consecutive 4 days,recovery time of lower limbs and the pathologic changes in the spinal cord by method of microscopy and electron scope were observed by a blind investigator.Results TFL in group LB31was significantly longer(P <0.05)and hind legs motor function and recover time pro-longed obviously in group LB31;Edema vacuolar degeneration and interstitial edema in white matter of posterior horn of spinal cord were found in group LB31.The histopathologic score was obviously higher in group LB31 compared with other groups (P <0.05).On transmission electroscope,differ-ent levels of ultrastructural changes were found in all groups except in group S .Typical changes included interstitial edema ,loosening of the laminae of myelinated fibers , axon swelling and local degeneration with most serious injury in group LB31.Conclusion The spi-nal injury following intrathecal local anesthetics mixtures was more serious with higher concentration of lidocaine in the mixture.Lidocaine concentration lower than 5% mixed with 1.25% bupivacaine intrathecally induces no obvious spinal neurotoxicity.

Hermanski-Pudlak Syndrome ; complications ; Humans ; Immunologic Deficiency Syndromes ; complications ; Male ; Middle Aged ; Red-Cell Aplasia, Pure ; complications