Objective To observe the influence of electroacupuncure(EA) at different therapeutic time windows on the effects of inosine on neuronal apoptosis and expression of heat-shock-proteins-70 (HSP70)after focal cerebral ischemic reperfusion injury (CIRI) in rats.Methods The model was established by ligation of the artery for 2 hour.EA was delivered to “baihui” and “dazhui” through acupuncture needles 0.5 hr,2 hr,6 hr and 12 hr respectively following MCAO.The expression level of HSP70 and the number of apoptotic cells were examined by immunohistochemical technique and TUNEL,comparing the average optical density of HSP70 and the number of apoptotic positive cells of cortex penumbra and hippocampus CA1 area in rat brain.Results Compared with the model group,the average optical density of HSP70 in every EA group was increased in apoptotic positive cells of cortex penumbra and hippocampus CA1 area 0.5 h (89.98± 6.55),(128.73 ± 8.03),2 h (90.96±6.38),(132.25±8.78),6 h (93.71±6.12),(132.58±7.04),12 (96.19±7.30),(133.57±6.19)and the number of apoptotic positive cells of cortex penumbra in every EA group was decreased 0.5 h (1.80±0.84),2 h (3.40± 1.14),6 h (5.00± 1.00),12 h (5.00±2.45).The number of apoptotic cells of hippocampus CA1 area was decreased just in the EA group of 0.5 h (1.60± 1.89).Conclusion EA could increase the expression level of HSP70 and at the same time decrease the number of apoptotic cells,EA plays an important part in protecting the neuronal cells of brain after focal cerebral ischemic reperfusion injury.The acupuncture treatment should be taken as far as early.

Objective To observed the clinical efficacy of captopril combined with amlodipine in hypertension patients of the influence of high noise.Methods A total of 150 hypertension patients who worked in ＞ 110 dB noise were divided into treatment group 1 (75 cases) and treatment group 2 (75 cases) by random digits table.Patients simply took enalapril in treatment group 1,patients took enalapril combined with amlodipine in treatment group 2.Selected 75 cases common hypertension patients were control group,they took enalapril combined with amlodipine.A year later,compared with three groups of plasma renin activity (PRA) and clinical efficacy.Results The PRA and efficient rate had no significant difference among three groups in half ayear (P ＞0.05).A year later,the PRA and efficient rate in treatment group 2 and treatment group 1 had obvious difference [(1.89 ± 0.37) ng/ (ml· h) vs.(2.40 ± 0.38) ng/ (ml· h),90.67 ％ (68/75) vs.72.00 ％ (54/75)] (P ＜ 0.05 or ＜ 0.01).But compared with control group no significant difference (P ＞0.05).Conclusion The clinical efficacy of captopril combined with amlodipine in hypertension patients is more apparent.

Objective To explore the value of laparoscopic operations for early-stage cervix cancer. Methods Eighteen patients with Ⅰ b～Ⅱ a astage cervix cancer underwent extensive total uterectomy combined with pelvic lymphadenectomy under laparoscope (17Ⅰ b cases, 1Ⅱ a case). Concurrently, another 19 patients with the same diagnosis and approximately the same clinical features as the abovementioned 18 cases, as the control group, were treated by open extensive total uterectomy combined with pelvic lymphadenectomy. The operation time, intraoperative blood loss, postoperative recovery and complications of two groups were compared. Results No statistically significant differences were found on mean operation time between the laparoscopy group (272.8 min?80.3min) and open group (226.5 min?66.8min) (t=1.921,P=0.063). The number of removed lymph nodes was 15.6?5.1 in laparoscopy group and 16.8?5.7 in open group, without statistically significant differences (t=0.674,P=0.505). The recovery time of intestine functions in laparoscopy group (34.2 hours) was obviously shorter than that of open group (60.7 hours) (P

Objective To observe the effects of a-eating stroke constipation with ZengYeChengQi Decoction (ZYCQD) and BuYangHuanWu Decoction(BYHWD). Methods 86 cases of stroke constipation were recruited to receive ZengYeChengQi Decoction (ZYCQD) and BuYangHuanWu Decoction (BYHWD) orally for one therapeutic course, and observe the therapeutic effects. Results Among 86 cases, 65 cases were totally recovery, 16 cases had obvious improvement, and 5 cases were inefficacy. The total effective rate was 94.19%. Conclusion Oral ZengYeChengQi Decoction (ZYCQD) and BuYangHuanWu Decoction (BYHWD) can improve constipation symptom score and improve the clinical therapeutic effect.

40 IU/L in one,and E2

Objective:To study the protective effect of polysaccharide from Rose roxburghii Tratt (RRTP-1) on neural stem cells damaged by glutamic acid (Glu). Method: RRTP-1 was obtained by ion exchange column chromatography and gel chromatography. The neural stem cells were cultured from the striata of fetal mice and damaged by glutamic acid to simulate hypoxic-ischemic brain injury. The cultures were divided into control group, glutamic acid group and test groups in which different doses of RRTP-1 were added with glutamic acid. The death rate of neural stem cells damaged by Glu and LDH leakage were observed. Results:The neural stem cells damage of test groups were alleviated,and the death rate of neural stem cells and LDH leakage of high dose test group were significantly lower than glutamic acid group. Conclusion:RRTP-1 has remarkable protective effect on neural stem cells damage.

Objective To evaluate the clinical efficacy of four different operative patterns of laparoscopic hysterectomy: laparoscopically assisted vaginal hysterectomy (LAVH), laparoscopically introfasial subtotal hysterectomy (LISH), laparoscopically subtotal hysterectomy (LSH) and laparoscopically total hysterectomy (LTH). Methods A retrospective analysis on 2272 cases of laparoscopic hysterectomy was carried out, including operating time, blood loss, complication and postoperative recovery. Results For the two groups which preserved cervix, LISH was performed in 1323 cases. The operating time was (91?21) min, blood loss (93?23) ml, complication rate 4.1%. LSH was conducted in 229 cases, with an operating time (70?18) min, blood loss (69?17) ml, complication rate 0. The difference between the two groups was significant (all P0.05). All patients recovered well postoperatively. Conclusions The four operative patterns are ideal for hysterectomy. Young patients should be operated with laparoscopic hysterectomy with preservation of cervix, old patients or patients with CIN should be operated with excision of cervix.

BACKGROUND: Insulin resistance and impaired insulin secretion are the key complications of polycystic ovary syndrome (PCOS). The incidences of insulin resistance syndrome (IRS), impaired glucose tolerance (IGT) and diabetes mellitus have been clearly shown to be race-related, thus the association between polycystic ovary syndrome and insulin resistance was race-related.OBJECTIVE: To analyze the correlation of insulin resistance with body mass index (BMI), blood lipids and plasma glucose in Tibetan patients with PCOS.DESIGN: A prospective study based on Tibetan population.SETTING: Department of Cardiology, General Hospital of Chinese PLA; Second Department of Internal Medicine,General Hospital of Tibetan Military Area Command of Chinese PLA.PARTICIPANTS: Thirty-six Tibetan PCOS patients, aged 25-42 years with a mean age of (32±5) years were selected from the Second Department of Internal Medicine and Department of Gynecology, General Hospital of Tibetan Military Area Command of Chinese PLA. Another 36 female inpatients were selected as the controls (control group), aged 24-35 years with a mean age of (30±5) years.METHODS: The disease histories were collected and general conditions were recorded. Oral glucose tolerance test (OGTT, 75 g) was conducted for all the subjects. All the subjects werefasted for 12 hours to collect venous blood samples to detect the levels of hormone, fasting insulin, total cholesterol, triglyceride, high-density lipoprotein cholesterol (HDL-C) and low-density lipoprotein cholesterol (LDL-C). Ovary was detected with echocardiography. The insulin resistance index was calculated by the homeostasis model assessment (HOMA-IR) was calculated by the formula:fasting insulin (mlU/L)×FPG (mmol/L)/22.5. The blood pressure, BMI and levels of blood lipids, plasma glucose and insulin were compared between the PCOS group and control group.MAIN OUTCOME MEASURES: Blood pressure, BMI and levels of blood lipids, plasma glucose and insulin were observed in both groups.RESULTS: All the 36 PCOS patients and 36 controls were involved in the analysis of final results. ① The BMI, systolic blood pressure and diastolic blood pressure in the PCOS group were higher than those in the control group (t =3.426 5,2.448 4, 2.212 5, P ＜ 0.05-0.01). ② The levels of total cholesterol, triglyceride and LDL-C in the PCOS group were higher than those in the control group (t=2.104 9, t =2.304 7, 2.400 0, P＜ 0.05-0.01), whereas the level of HDL-C was lower that in the control group (t =4.800 0, P＜ 0.01). ③ The levels of fasting insulin and 2-hour plasma glucose and HOM in the PCOS group were higher than those in the control group (t =7.809 5, 12.365 0, 2.789 9, P＜ 0.01).CONCLUSION: Tibetan PCOS patients present a clustering of atherosclerotic risk factors, including obesity, adverse lipid profile, hypertension, hyperglycemia, etc.

Objective To analyze the effect of nimodipine on prevention and treatment of patients with severe burn sepsis. MethodsTwenty-eight cases were randomized into nimodipine treatment group (n = 14) and routine group (n = 14). Besides general chnical therapy scheme treatment,the treatment group was administrated with saline plus nimodipine (15 μg/(kg · h)), while the routine group was given saline only. The Plasma concentration levels of total calcium (Ca2 +), free calcium (iCa2 +), C-reactive protein (CRP), procalcitonin (PCT), tunmor necrosis factor α (TNF-α), brain natriuretic petide (BNP) and interleukin-6 (IL-6) were measured. The hemodynamic indices of patients with severely burn sepsis were determined by transesophageal echo-Doppler device,hemodynamic indices including aortic blood flow (ABF), left ventricle ejection time (LVET), max acceleration (ACC), total systemic vascular resistance (TSVR). Mean artery pressure (MAP) and heart rate (HR) were measured. ResultsABF, ACC, LVET and TSVR were improved after therapy in the routine group(t = 3.93,3. 37,3.75,7.02) (P ＜ 0.01 or 0.05). ABF, ACC, LVET and TSVR were mproved after therapy in the treatment group (t =6.46,4.98,6.29,4.60) (P ＜0.01 or 0.05). ABF, ACC and LVET were increased after general clinical therapy scheme and was markedly increased in the treatment group (t = 2.29,2.07,2.23)(P＜0.01～0.05).TSVR was decreased after general clinical therapy scheme and was markedly decreased in the treatment group (t =2.14) (P＜0.05). Plasma TNF-α, IL-6 and CRP levels were decreased after therapy in the routine group (t =2.38,2.29,2.45)(P＜0.01 or 0.05). Plasma TNF-α, IL-6 and CRP levels were decreased after therapy in the treatment group (t =4.04,4.04,2.56)(P ＜0.01or 0.05). Plasma PCT and BNP levels were decreased in the treatment group (t =5.45,2. 44) (P ＜ 0.01 or 0.05). TNF-α, IL-6, CRP, PCT and BNP were decreased after generalclinical therapy scheme and was markedly decreased in the treatment group (t = 2.20,2.17,2.19,2.17,2.61) (P ＜0.01 or 0.05). Plasma Ca2+ and iCa2+ levels were decreased. However,MAP were increased in the two groups, although no difference was found between the two group(P ＞0.05). ConclusionsEarly applying of calcium channel blockers (CCB) can markedly inhibit the degree of inflammatory medium in serum, thereby preventing the development of excessive inflammation response and subsequent multiple organ dysfunction syndrome associated with severely burned sepsis. It can effectively inhibit intracellular Ca2+ overload caused by myocadial infection injury,which may contribute to the effect of acupuncture in reducing myocardial injury and protecting myocardial performance and improving prognosis.

BACKGROUND: Carotid intima-media thickness (IMT) is the substitution index of coronary atherosclerosis, but the precise relation between IMT and coronary artery disease (CAD) is still unknown.OBJECTIVE: To investigate the role of carotid IMT in coronary atherosclerotic heart disease (CAHD).DESIGN: A case analysis.SETTING: Department of Cardiology, Chinese PLA General Hospital.PARTICIPANTS: The study was carried out in the Department of Cardiology, Chinese PLA General Hospital from January 2000 to January 2002. Totally 285 inpatients aged 33-74 years, mean (54.48±9.44)years with suspected CAHD, including 164 males and 121 females, were involved in this study. According to angiographic results, they were divided into 4 groups: 0-vessel group, 1-vessel group, 2-vessel group and 3-vessel group.METHODS: ①The weight and height of patients were measured, and body mass index (BMI) = weight (kg)/height (m)2.②In the supine position, the blood pressure of left arm were measured. ③Venous blood was sampled todetect blood lipid. ④High-resolution B-mode ultrasound and 10 MHz linear transducer was used to evaluate the common carotid artery,internal carotid artery, external carotid artery and carotid bifurcation.Carotid IMT ≥ 1.0 mm was defined as carotid atherosclerosis. The results of coronary angiography by standard Judkins technique showed that, CAHD extent (0, 1, 2 and 3) was determined by the number of diseased vessels,where luminal stenosis was ≥ 50%. Left stem stenosis ≥ 50% was defined as 2-vessel while left stem merging right coronary affection were taken as 3-vessel.MAIN OUTCOME MEASURES: ①IMT values at different detection sites of the patients with different CAHD lesions; ②The predictors of the CAHD severity;③The predictive values of IMT at different carotid sites on CAHD; ④The relationof different risk factors and IMT.RESULTS: Totally 285 patients were involved in the result analysis.①IMT values at different detection sites of CAD patients: The chi square test demonstrated that, left and right carotid bifurcations took the maximal F value among groups (left: 65.64, right: 63.24). And the IMT values of left and right carotid bifurcations were elevated with the increasing CAHD severity. ②The predictors of the CAHD severity: The regression analysis implied that male, high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C), and IMT of left carotid bifurcation, right carotid bifurcation, right internal carotid artery and right external carotid artery were the independent predictors of CAHD severity, while left and right carotid bifurcation were the most important (t=3.61, P=0.391; t=2.58,P=0.247). ③The predictive values of IMT at different carotid sites on CAHD: The left and right carotid bifurcations also found the maximal positive predictive value for detecting CAHD (left: 89.9%, right: 88.8%).④Multivariate regression analysis revealed that age, BMI, cholesterol,triglyceride, HDL-C and LDL-C were the risk factors of left carotid bifurcation atherosclerosis.CONCLUSION: IMT in the carotid bifurcation is related significantly with the presence and extent of CAHD. They share common pathological and hemodynamic basis. Carotid bifurcation IMT may be used as a surrogate marker for CAHD.