OBJECTIVE To explore the outcome of endoscopic transnasal management of inverted papilloma of nasal cavity and sinuses. METHODS The clinical data of 28 cases with inverted papillomas of nasal cavity and sinuses treated via nasal endoscope were studied retrospectively. RESULTS All of the patients were followed up from 7 months to 5 years. Only 6 cases(21%) recurred. Of the 6 cases, ethmoid and sphenoid sinuses were involved in 2 cases and maxillary sinus was involved in 4 cases. CONCLUSION Endoscopic transnasal resection of inverted papilloma is a good approach for the treatment of patients with inverted papilloma of nasal cavity and sinuses.

To explore the originating sites,clinical manifestations,imaging features,pathologic findings,treatments and prognosis of low-grade fibromyxoid sarcoma.Among 54 cases,6 came from our hospital and the other 48 cases were reported in literatures from 1998 to 2012 in China Knowledge Resource Integrated Database (CNKI).Their data were reviewed for analysis.The usual presentation was a slowly growing,painless mass commonly located in extremities.The optimal treatment of extended resection could significantly reduce recurrence and improve survival.And a long-term follow-up was necessary.

Objective To observe the survival and the differentiation of grafted bone marrow cells (BM-MNCs) in host myocardium.To observe whether BM-MNCs transplantation can potentially cause arrhythmia and whether the BM-MNCs transplantation can alter the spatial distribution of connexins,important mediator for arrhythmia genesis after myocardial infarction.Methods Acute myocardial infarction (AMI) was induced by left anterior descending coronary artery (LAD) ligation in hybrid canine.BM-MNCs suspension was prepared by density centrifugation.The BM-MNCs were labeled with CM-DiI.Sixteen hybrid canines were randomly divided into transplantation group and control group.BM-MNCs (transplantation group,n =10) or saline (control group,n =6) were intracoronarily infused into infarction related artery at 2 hours after AMI.At 6 weeks after AMI,ventricular fibrillation (VF) was induced in infarct area and periinfarct area.The effective refractive period (ERP) of different areas in myocardium was assessed and the expression of connexin 43 (Cx43) was assessed by immunohistochemical staining.Results Six weeks after the BM-MNCs transplantation,CM-DiI labeled BM-MNCs were mainly located within periinfarct and infarct area.Some BM-MNCs were positive for Cx43.Combined "CM-DiI and FITC" in images were observed.VF was induced in 2 out of the 10 canines in transplantation group and in 2 out of the 6 canines in control group in infarct area.VF was not induced in periinfarct area of both groups.The ERP of infarct area ((85.0 ± 9.3) ms vs.(90.0 ± 7.1) ms,P ＞ 0.05),periinfarct area (87.8 ± 9.4 vs.90.0 ± 7.1,P ＞ 0.05) and normal area (85.0 ± 12.0 vs.88.3 ±9.4,P ＞0.05) was similar between transplantation group and control group.The expression of Cx43 in normal area was similar between transplantation group and control group (3543.7±446.0 vs.3 431.7 ± 421.5,P＞ 0.05).The expression of Cx43 in periinfarct area of transplantation group was significantly higher than that in control group (2 312.5 ±412.0 vs.1 356.2 ± 332.7,P ＜0.05),but was still much less than in normal area (2 312.5 ± 412.0 vs.3 543.7 ± 446.0,P ＜ 0.05).The expression of Cx43 in infarct area was similar between transplantation group and control group (327.0 ± 98.7 vs.311.3 ± 78.7,P ＞ 0.05).Conclusions The implanted BM-MNCs could survive in the infarcted lesion and differentiate into cells expressing Cx43.In transplanted group,VF was not induced in periinfarct area.ERP of infarct area,periinfarct area and normal area is similar between two groups.The expression of Cx43 in periinfarct area was significantly higher in transplantation group than that in control group.

ObjectiveTo investigate the effect of Chaihu Guizhitang on triple-negative breast cancer （TNBC） cells based on hypoxia-inducible factor-1α （HIF-1α）/vascular endothelial growth factor A （VEGFA） signaling pathway. MethodTNBC xenograft model was established and the cells were randomized into model group， capecitabine group （0.2 mg·kg^-1）， Chaihu Guizhitang low-dose group， medium-dose group， and high-dose group （10.62， 21.23， 42.46 g·kg^-1）， with 10 mice in each group. After 21 days of medication， the content of tumor necrosis factor-α （TNF-α） in serum was detected by enzyme-linked immunosorbent assay （ELISA）. The expression of HIF-1α mRNA was detected by real-time fluorogenic quantitative polymerase chain reaction （real-time PCR）. Immunohistochemistry （IHC） was employed to detect the expression of HIF-1α， TNF-α， and VEGFA in tumor tissues， and CD34 staining to examine the angiogenesis in tumor tissues. Microvessel density （MVD） was calculated， and the protein expression of HIF-1α， VEGFA， and epidermal growth factor receptor （EGFR） in tumor tissues was measured by Western blot. ResultCompared with the model group， the rest four groups showed low levels of TNF-α （P<0.01）， HIF-1α mRNA （P<0.01）， expression of HIF-1α， TNF-α， VEGFA， and CD34 in cells， and MVD （P<0.05， P<0.01）， and low protein levels of HIF-1α， VEGFA， and EGFR （P<0.01）. Compared with capecitabine group， medium-dose and high-dose Chaihu Guizhitang decreased the level of TNF-α （P<0.01）， HIF-1α mRNA （P<0.01）， expression of HIF-1α， TNF-α， and VEGFA in cells （P<0.01）， CD34 expression， MVD， and protein levels of HIF-1α， VEGFA， and EGFR （P<0.01）. ConclusionChaihu Guizhitang may inhibit the angiogenesis in TNBC cells by regulating the expression of HIF-1α/VEGFA signaling pathway， thus exerting anti-tumor effect.