Objective To establish an assay for the quality control of Chanhoukang Granules. Methods Radix Astragali, Radix Codonopsis, Radix Salviae Miltiorrhiae, Pericarpium Citri Reticulatae, Radix Rehmanniae preparata in Chanhoukang Granules were identified by TLC; paeoniflorin content in Chanhoukang Granules was determined by HPLC. Results The relevant spots in Radix Astragali, Radix Codonopsis, Radix Salviae Miltiorrhiae, Pericarpium Citri Reticulatae, Radix Rehmanniae preparata can be identified by TLC. The content of paeoniflorin in Raidix Paeoniae Alba can be determined by HPLC. The linearity of paeoniflorin was good in the range of 0.044 2～ 0.353 6 ? g(r=0.999 9)The average recovery of paeoniflorin was 97.56 % with RSD=1.18 % . Conclusion The established method is simple, feasible and repeatable, and can be used for the quality supervisory of Chanhoukang Granules.