Presently,studies of onset,development and tumor drug resistance of osteosarcoma were few.Further studies on tumor animal models can provide a promising basis for treating osteosarcoma.Commonly used animal models of osteosarcoma include mouse,rat,rabbit and dog.Organs of rats are similar to human,so does genotype.Dogs can spontaneously develop osteosarcoma,which is similar to human,and are frequently used as spontaneous tumor models.However,there are some differences in biological features,pathogenic mechanism and histology.With the development of studying,there will be more and more new animal models in accordance with human osteosarcoma characteristics.The present study served to review methods of establishing models,characteristics and detailed application of animal models of osteosarcoma.

Objective To determine the intra-and inter-tester reliability of a dynamometer in assessment of knee joint position senses (JPS).Methods Sixteen healthy volunteers (intra-rater N =6,inter-rater N =10) were examined with regard to intra-and inter-tester reliability of knee JPS by using Biodex System 3 dynamometer,measured by passive replication test,with subjects passively returning to the initial positions at 30°,45° and 60° knee flexion.The absolute error (AE) angle was recorded.Both intra-and inter-tester reliability studies involved two testers.Results The intra-tester reliability of the AE were moderate reliable at 30°,45°and 60° knee flexion,and the values of intra-class correlation coefficients (ICC) were 0.728,0.780 and 0.807,respectively.The values of Pearson's correlation coefficients for inter-tester reliability were 0.676,0.610 and 0.705,respectively (P ＜ 0.05).There was no significant difference between the AE values of dominance and non-dominant knees,test and retest,and in the AE values of 30°,45°and 60° knee flexion (P ＞ 0.05).The 95％ confidence upper limits of all subjects' AE values were less than 3°.Conclusion The major findings of this study suggest that the test-retest reliability of the knee JPS were moderate using Biodex System 3 dynamometer,and selecting a target angle of passive replication test can achieve the same purpose in 30° to 60°knee flexion.The clinical significance should be taken into consideration when the AE changes were greater than 3 degrees.

Objective To observe and compare the functional outcomes of early rehabilitation of persons who received primary bilateral or unilateral total knee arthroplasty (TKA).Methods Sixty-eight subjects were divided into a unilateral TKA group (36 cases) and a bilateral TKA group (32cases).All received the same professional rehabilitation program during an average postoperative hospital stay of (15.00 ± 3.71 ) days.At discharge,a visual analogue scale (VAS) was used to rate pain.Active range of motion (AROM) of the involved knee,knee injury and an osteoarthritis outcome score (KOOS) were also assessed.The KOOS and knee society score (KSS) were evaluated at follow-up about 11 months later.Results There was no significant difference between the two groups in any assessment at discharge,nor in the KOOS and KSS-Ⅰ assessments at follow-up.The KOOS of both groups at follow-up had improved significantly compared with that at discharge.The KSS-Ⅱ results in the bilateral group were slightly better than those in the unilateral group at follow-up,and that difference was significant.Conclusions Bilateral TKA patients and unilateral TKA patients had the same clinical outcomes after early postoperative rehabilitation,but the functional outcomes of bilateral TKA patients were better than those of unilateral TKA patients a year later.

目的探讨间歇性气压治疗在下肢创伤早期康复中消除肿胀的作用。方法将 65例患者分为对照组和治疗组。对照组33例,采用常规康复治疗。治疗组32例,在采用常规康复治疗同时进行间歇性气压治疗。结果治疗组患肢肿胀及疼痛程度减轻明显优于对照组。结论下肢创伤患者在康复过程中配合间歇性气压治疗减轻肿胀和疼痛,对促进患者配合早期运动起着积极作用。

Objective:To observe the effects of 8-week interval anaerobic training on the respiratory function, airway inflammatoion and exercise performance in the asthmatic children,and to provide theoretic and practical evidence for special exercise prescription for the asthmatic children.Methods:Forty-six mild asthmatic children were willingly divided into exercise group (n=23)and control group (n=23).The children in exercise group conducted 8 weeks interval anaerobic training while the children in control group maintained intrinsical lifestyle.Before and after experiment,the respiratory function,airway inflammatory reaction and exercise performance were determined by pneumonometer,nitric oxide determinator and cycle ergometer.Results:One patient of each group was lost to follow up.509 persons in exercise group finished the training;11 persons had adverse reaction and the incidence was 2.2%.After experiment,compared with before experiment,the peak expiratory flow (PEF)in exercise group after experiment was increased (P <0.05),the fractional exhaled nitric oxide (FeNO)was decreased (P <0.05), the mean power (MP)and peak power (PP)in 30 s Wingate test were raised (P <0.05);there were no significant differences of all indicators in control group (P >0.05).Compared with control group after experiment,all indexes above (PEF,FeNO,MP and PP)in exercise group existed statistical differences (P <0.05).Conclusion:Interval anaerobic training has safetiveness and effectiveness under proper supervision and could improve the respiratory function,reduce the airway inflammation and enhance the exercise performance in the mild asthmatic children.

Intervertebral disc degeneration is one of the orthopedic common diseases , its complex physiopathological changes are regulated by many factors .The matrix metalloproteinases ( MMPs) and its regulatoryl factors play a very important role , and the factors that affect the role of MMPs in intervertebral disc degeneration interact with each other .So MMPs have gradually become one of the hot spot of intervertebral disc degeneration in recent years .In this paper , we first analyze the factors that cause the degeneration of intervertebral disc .Then we summarize the relationship between the expression of MMPs and the degeneration of intervertebral disc ac -cording to the different function of MMPs members in the family .Finally we state that MMPs can have an effect on the degeneration of intervertebral disc by the regulation of gene polymorphism and the related factors .

Objective To explore the influence of 8-week aerobic exercise in heart rate variability (HRV)in obese adolescents and to provide the evidence for formulating special exercise prescription.Methods Forty male obese adolescents (BMI≥25 kg·m-2 )were randomly divided into exercise group (n=20)and control group (n=20). The subjects in exercise group conducted a 8-week (five times/week,50-60 min)aerobic exercise while those in control group maintained their routine lifestyle. The body composition, hemodynamic parameters including heart rate(HR)and blood pressure and HRV parameters including total power (TP),normalized low frequency (LFn), normalized high frequency (HFn)and LFn/HFn ratio were measured before and after experiment.Results There were no significant differences of the indicators of the subjects between two groups before experiment (P>0.05). At the end of experiment,compared before experiment,the fat mass(FM),the percentage of body fat(BF%), the rest HR,SBP and DBP were decreased (P<0.05 or P<0.01);the TP and HFn were increased(P<0.01);the LFn and LFn/HFn ratio were reduced (P<0.01)in exercise group after experiment;but the indexes of the subjects in control group showed no significant changes (P>0.05).Compared control group,the FM,the BF%, the rest HR,SBP,DBP,LFn and LFn/HFn ratio were decreased while the TP and HFn were increased (P<0.01)in exercise group after experiment.Conclusion Aerobic exercise may effectively reduce the sympathetic activity and increase the vagal tone in the obese adolescents.

s:Bone allografts have been successfully used in repairing bone defect caused by tumor, infection or trauma. The mechanism of bone-healing of allografts has been widely studied. The article reviews the factors affecting the bone-healing of allografts.

AIM:To investigate the changes of brain-derived neruotrophic factor(BDNF),tyrosine kinase B(TrkB) in rat cortex and hippocampus with chronic immobilization stress and the influence of three Chinese formulas(Xiaoyaosan,Sijunzitang,Jinkuishenqiwan) on them.METHODS:Chronic immobilization stress method(180 min daily,repeated 7 days or 21 days) was taken,and the changes of BDNF,TrkB in rat forehead cortex and hippocampus CA1 were measured by immunohistochemistry integrated image analysis.RESULTS:The contents of BDNF in rat forehead cortex and hippocampus CA1 were obviously lower in the model group of 7 days and 21 days than those in the normal control group(P

Objective:To investigate the protective effect of lactoferrin(Lf) on lung injury in mice exposed to irradiation.Methods:C57BL/6 J mice were randomly divided into control group, 15 Gy irradiation group (IR group) and lactoferrin combined 15 Gy irradiation group (Lf+ IR group), with 5 mice in each group. The mice in the Lf+ 15 Gy group drank lactoferrin solution (10 mg/ml) from 3 days before irradiation and contained the whole experiments. Then, single chest 15 Gyirradiation was performed both in the IR and Lf+ IR groups. The body weight and other characteristics were monitored during the experiment. The mice were killed at day 14 after irradiation. The lung histopathology was observed by HE staining. Serum inflammatory cytokine such as HMGB1, TNF-α, IL-1β and IL-6 was determined by ELISA method . The expression of inflammatory related protein in lung tissue including HMGB1, TLR4, MyD88 and NF-κB were performed by immune histochemistry and Western blot method.Results:Compared with the control group, lung weight was significantly increased ( t=3.20, P<0.05), pulmonary hyperemia and inflammatory cell infiltration was observed in the IR group. Exposure also significantly increased serum level of TNF-α[(291.80±5.49) vs.(332.25±22.18)pg/ml]( t=3.07, P<0.05), up-regulated the expression of inflammatory related protein in lung tissue ( t=4.04, 4.78, 3.77, 6.14, P<0.05). Lactoferrin intervention (Lf+ IR group) significantly decreased lung weight ( t=2.18, P<0.05), alleviated histopathologic changes, decrease serum levels of HMGB1, TNF-α and IL-1β ( t=4.67, 2.97, 3.49, P<0.05). On the other hand, lactoferrin intervention decreased the positive cell number of HMGB1 and NF-κB, and down-regulated the protein expression of HMGB1, TLR4, MyD88 and NF-κB in lung tissues, with significant difference with the IR group ( t=8.06, 9.80, 3.07, 5.56, P<0.05). Conclusions:Lactoferrin plays the protective effect of radiation-induced lung injury through the downregulation of inflammatory response, such as HMGB1/TLR4/MyD88/NF-κB signaling pathway.