Endothelial progenitor cells (EPCs) are a precursor cell of endothelium that has the ability of differentiation and proliferation. In given conditions, EPCs can be mobilized to the ischemic tissue to participate in repairing the injured blood vessels and angiogenesis, and EPCs are regarded as a marker of vascular reparative capacity. Studies in recent years have indicated that the changes of EPC number play an important role in the occurrence and prognosis of cerebral ischemia. As a potential therapeutic strategy, EPCs may be used in ischemic cerebrovascular disease. This article reviews the application progress of EPCs in ischemic cerebrovascular disease.

objective: To offer a model for reference on the operating guideline of ART Institution Ethics Committee to regulate their conducts on ethical supervision in China. Method: Generalizing the mode of committee' action out of duty according to the scientific principle in clause. Conclusion: The suggestion draft, based on committee's practical exploration of ethics, integrates the international practice and external experience for reference properly, which is overall and has stronger operating quality and can draw lessons from the meaning.

Objective: To study the chemical constituents of ethyl acetate extract from domestic Pfaffia glomerata. Methods: The compounds were isolated and purified by silica gel, reversed-phase, Sephadex LH-20 column chromatography, and their structures were elucidated by spectroscopic analysis as well as chemical methods. Results: Twenty compounds were identified as 1-undecanol (1), oleic acid (2), β-sitosterol (3), 2-hydroxy-4,6-dimethoxyacetophenone (4), oleanolic acid (5), pfaffianol A (6), benzene-1,4-diol (7), vanillic acid (8), iresinone (9), ethylcaffeate (10), oleanoicaeid-28-O-β-D-glucopyranosyl ester (11), allantoin (12), ajugasterone-C (13), β-ecdysterone (14), iresinoside (15), adenine (16), oleanolic acid 3-O-β-D-glucuronopyranoside (17), ficusoside B (18), pfaffiaglycoside B (19), and β-D-Glucopyranosyl-3-(O-β-D-glucopyranosyloxy)-oleanolate (20). Conclusion: Compounds 7-10, 15, 17, 18, and 20 are isolated from this plant for the first time.

Objective: To study the chemical constituents of Gynostema pentaphyllum. Methods: The chemical constituents were isolated and purified by silica gel, polyamide, and Sephadex LH-20 chromatography. Their structures were elucidated by physicochemical properties and spectral analysis. Results: Seventeen compounds were isolated and identified as dodecanoic acid (1), β-sitosterol (2), 3, 3', 5-trihydroxy-4', 7-dimethoxyflavanone (3), 1-2-benzenediol (4), 3'-O-methyltaxifolin (5), quercetin (6), rhamnetin (7), α-spinasterol-3-O-β-D-glucopyranoside (8), 3, 4-dihydroxy benzoic acid (9), narcissoside (10), L-rhamnosemono-hydrate (11), malonic acid (12), β-ethoxy-rutinoside (13), rutin (14), ombuoside (15), ginsenoside Rb1 (16), and β-daucosterol (17). Conclusion: Compound 1, 3-5, 7, 9, 10, and 12 are obtained from G. pentaphyllum for the first time.

OBJECTIVETo investigate the clinical results and the superiority of minimally invasive sinus tarsi approach andABSTRACT Objective :To investigate the clinical results and the superiority of minimally invasive sinus tarsi approach and external fixation with calcaneal locking plates for the treatment of the intra-articular calcaneal fracture.

METHODSFrom July 2011 to August 2012, 12 cases (13 feet) were treated by sinus tarsi approach and external fixation with calcaneal locking plates. There were 10 males and 2 females, aged from 25 to 48 years old (means 35 years old). According to Sanders classifi- cation system, there were 7 feet with type II, 6 feet with type III. The Böhler angle and Gissane angle were compared before and after operation. The clinical results were evaluated with the Maryland foot score system.

RESULTSAll 12 cases were followed up from 6 to 14 months (means 10 months). There were no incision complications. Fracture healing time was from 3 to 6 months (means 4 months). The Böhler angle increased from preoperative (11.19 +/- 5.69) degrees to postoperative (30.13 +/- 7.52) degrees; The Gissane angle increased from preoperative (108.31 +/- 10.35) degrees to postoperative (118.99 +/- 8.94) degrees. According to Maryland foot score system, 8 feet got an excellent result, 3 good, 2 fair.

CONCLUSIONOpen reduction by sinus tarsi approach and external fixation with calcaneal locking plates is an effective method for treatment of Sanders II, III calcaneal fracture. It can obtain good reduction and stable fixation with minimal invasion and lower complications.

Adult ; Ankle Joint ; surgery ; Bone Plates ; Calcaneus ; injuries ; surgery ; External Fixators ; Female ; Fracture Fixation ; instrumentation ; methods ; Humans ; Intra-Articular Fractures ; surgery ; Male ; Middle Aged ; Minimally Invasive Surgical Procedures ; methods ; Treatment Outcome

ObjectiveTo design a simple leg-sliding rehabilitation equipment for patients with lower limb dysfunction who need to exercise their leg muscles in bed at the early stage of rehabilitation. MethodsThe mechanism scheme was designed using the crank slider transmission system. The kinematic model was established and the structural dimensions were determined according to the human joint mobility, analysis and verification were then implemented; and the strutural strength of the machanism was verified, and based on the analysis, a protype was built to verify the feasibility of proposed scheme. ResultsThe range of joint activities of the proposed mechanism was in line with the normal human joint activities, the structural strength met the requirements, the prototype operated smoothly, and the actual running speed was basically consistent with the theoretical planning speed. ConclusionThis design could meet the needs of rehabilitation training for bedridden patients with lower limb dysfunction at the early stage of rehabilitation. It has the advantages of small size and light weight, which provides reference value for promoting the development of miniaturized and lightweight lower extremity rehabilitation equipment.

The resection can be performed in about 20 percent of patients with primary hepatocellular carcinoma(PHC) in clinic. However, with the development of interventional treatment such as transcatheter arterial chemoembolization(TACE), better therapeutic effect has been achieved by two-stage resection such as TACE in patients with unresectable PHC. At present, surgical resection is still regarded as the first choice of PHC, and the principle of comprehensive therapeutic solutions around surgical operation has been established. The pretreatment of TACE is an important measure to improve the therapeutic effect in PHC.This paper discussed the mechanism, effect and value of TACE in pretreatment of unresectable PHC. The selection of operative opportunity, sign, and ways after TACE and the factors affecting prognosis of two-stage resection were also reviewed.

OBJECTIVETo observe the effect of Cordyceps sinensis and artemisinin in preventing recurrence of lupus nephritis (LN).

METHODSSixty-one LN patients, who had no activities by corticosterone and cyclophosphamide (CTX) impacting therapy were randomly divided into two groups. The 31 cases in the treated group were given Cordyceps powder 2-4 g/d before meal and artemisinin 0.6 g/d after meal in three portions orally taken for 3 years. The 30 patients in the control group were treated with tripterygiitotorum and/or Baoshenkang tablet. The consecutive observation lasted for 5 years to monitor the clinical manifestations of lupus and laboratory indexes including blood creatinine, creatinine clearance rate (CCr) and antinuclear antibodies (ANA).

RESULTSThe therapeutic effect showed markedly effective in 26 cases (83.9%), effective in 4 (12.9%) and ineffective in 1 (3.2%) in the treated group, while in the control group, the corresponding numbers were 15 (50.0%), 8 (26.7%) and 7 (23.3%), the difference between the two groups in markedly effective rate was significant (P < 0.01). In the treated group, C3 level was stabilized at above 1.21 +/- 0.20 g/L, which was over the normal range, CCr was unchanged as compared before and after treatment, which was significantly different from that in the control group. Moreover, the side-effects occurred in the treated group was less.

CONCLUSIONCordyceps and artemisinin could prevent the recurrence of LN and protect kidney function.

Adult ; Aged ; Anti-Infective Agents ; therapeutic use ; Artemisinins ; therapeutic use ; Complement C3 ; metabolism ; Cordyceps ; Creatinine ; blood ; Drug Therapy, Combination ; Drugs, Chinese Herbal ; therapeutic use ; Female ; Humans ; Lupus Nephritis ; drug therapy ; Male ; Middle Aged ; Phytotherapy ; Secondary Prevention ; Sesquiterpenes ; therapeutic use

OBJECTIVE:To find out the general rule in ware-house-in inspection for Chinese medicinal crop.METHO_ DS:135reports of consigned inspection for Chinese medicinal material were quantitatively analysed in linking with the pre?served specimens.RESULTS:Main specimens inspected included Radix Morindae Officinalis,Bulbus Fritillariae Cirrhosae,Herba Cistarchis and Semen Cuscutae.The main cause of those unqualified Chinese medicine was due to fake and impure ma?terials adulterated.The target items of adulteration were herbal pieces and other small size Chinese medicinal materi?als.CONCLUSION:Chinese medicine should be purchased from the source of original unprocessed materials.During taking delivery of goods,formal differentiation and scientific inspection process should be performed to prevent fake and unqualified medicine being stored and used,to ensure the product quality up-to-standard.

OBJECTIVE: To assess the influence of Qingdu (QD) capsule on the pharmacokinetic of HAART. METHODS: The 36 SD rats were divided into 3 groups. Rats in Group HAART were given, using an intragastric gavage needle, highly active antiretroviral therapy (HAART) containing zidovudine (AZT), 2',3'-dideoxy-3'-thiacytidine (3TC) and efavirenz (EFV). Group HAART+QD were given HAART and QD capsule simultaneously. Group HAART+QD (2 h interval) were given HAART and then given QD capsule 2 h later. Blood samples were collected at 0, 0.5, 1, 2, 3, 4, 6, 8, 10 and 12 h. AZT, 3TC, and EFV concentrations were tested with high performapce liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Pharmacokinetic parameters were compared among different groups. RESULTS: The t1/2 of AZT were statistically different among the three groups (P<0.05), Group HAART+QD (2 h interval) had the lease t1/2 of AZT than the other two groups. There were no statistical differences among groups for the AUC0-12, tmax, ρmax and CL of AZT, and all five parameters of 3TC and EFV. CONCLUSION: QU doesn't show influence on pharmacokinetic parameters of AZT+3TC+EFV regimen, but intention should be paid to individual differences.