1.Application of combination of parenteral nutrition with enteral nutrition in perioperative period of total gastrectomy
Chinese Journal of General Surgery 2001;0(08):-
ObjectiveTo determine the effects of combination of parenteral nutrition(PN) with enteral nutrition (EN) on patients with gastric cancer in perioperative period of total gastrectomy . MethodsIn 52 patients with advanced gastric cancer, 41 had PN support preoperatively and all 52 patients received combination of PN with EN after the total gastrectomy. Results48 of the 52 patients whose intestinal function was recovered within 3 days postoperatively. When using EN, 5 cases felt borborygmus,abdominal pain or mild diarrhea, but these symptoms decreased by slowing down of the infusion speed. The concentration of plasma albumin showed a marked increase on the 10th postoperative day(P
2.Study on the change of migration ability of dendritic cells induced by intermittent hypoxia and the mechanism of its pathway
Ke HU ; Guangwei WANG ; Yu YANG ; Guoyao XU
Chongqing Medicine 2016;45(30):4200-4202,4206
Objective By simulating the intermittent hypoxia(IH ) environment of human obstructive sleep apnea syndrome (OSAS) ,to reveal the effect of IH on migratory ability of human peripheral blood derived dendritic cells(DCs) ,and through the in‐tervention of RelB ,p38 expression in order to explore the possible mechanism of the change of DCs migration ability induced by IH . Methods DCs were divided into RelB ,p38 siRNA interfering and non interfering plasmid group before cultivation .The environment of hypoxia was created by a intermittent hypoxia cabin ,among them ,oxygen concentration was 0 .5% ,1 .5% ,5 .0% ,10 .0% ,hypox‐ia/reoxygenation time ratio was set as 1∶1 ,1∶3 ,1∶5 and 1∶9 ,while sustained oxygen was supplied to the contrast at a normal concentration of 21 .0% .The content of RelB and p38 was tested by Western blotting after culture in vitro ,migration ability of DCs was detected by invasion chamber .Results Compared with normoxia ,DCs under IH tended to have declined migratory ability , which was confirmed to be correlated with the average oxygen partial pressure level under IH .IH could promote the expression of RelB and p38 in DCs ,while the migratory ability of DCs was not reversed after intervening the expression of RelB and p38 .Conclu‐sion IH in vitro could cause a decline in migratory ability of DCs ,which may not be induced by activation of RelB or p38 in DCs .
3.Detection of E-cadherin gene promoter hypermethylation in plasma of breast cancer patients
Jingjie ZHANG ; Tao OUYAN ; Guoren DENG ; Wenhui WAN ; Guangwei XU
Chinese Journal of General Surgery 2000;0(11):-
Objective To evaluate the feasibility in early diagnosis, predicting therapeutic effect, recurrence monitoring by examining promoter hypermethylation for cancer-associated genes E-cadherin in cancer tissue and peripheral blood of breast cancer patients. Methods The tumor tissue, paracancer- tissue and the paired plasma were examined for aberrant methylation of E-cadherin gene by methylation-specific PCR in 42 cases of breast cancer and 10 cases of breast benign diseases. Results The incidence of promoter hypermethylation of E-cadherin in tumor tissues was 52.4% and the paired plasma were 33.3%. E-cadherin hypermethylation in plasma samples and tumor samples significantly correlated with each other ( P
4.Expression of cyclooxygenase-2 (COX-2) in hepatocellular carcinoma cell lines
Kun WANG ; Baocai XING ; Qingyun ZHANG ; Guangwei XU ;
Chinese Journal of General Surgery 1993;0(02):-
Objective To investigate the expression of cyclooxygenase-2 in hepatocelluar carcinoma cell lines. Methods RT-PCR and immunocytochemistry were use d to investigate the expression of cyclooxygenase-2 in 6 hepatocellular carcino ma cell lines. Result COX-2 mRNA expression was detected in five of six cell lines, and all six cell lines w ere positive for COX-2 protein expression. Conclusion COX-2 is expressed in hepatocellular carcinoma cell li nes, providing basis for the chemoprevention of hepatocelluar carcinoma.
5.Surgical treatment of adenocarcinoma of the middle/low rectum
Jin GU ; Ji ZHANG ; Yi WANG ; Guangwei XU
Chinese Journal of General Surgery 2001;0(10):-
0.05). Only tumor classification and cell differentiation were proved to be independent factor determing the outcome (P
6.High-Efficiency Retroviral Vector Containing Human Mutated Dihydrofolate Reductase cDNA and Its Expression in Murine Hematopoietic Progenitor Cells
Wenqing ZHANG ; Zuoliang XU ; Jie YU ; Guiguo ZHANG ; Guangwei XU ; Guilin WANG ; Shicheng ZHAO ;
Chinese Journal of Cancer Biotherapy 1995;0(02):-
A double-copy Moloney leukemia virus-based retroviral vector containing both the Neo~(R) gene and a mutant human dihydrofolate re-ductase(S31 mutation) cDNA was packaged into the Amphotropic packaging cell hne GP-EAM12( AM12), and a Amphotropic producer cell hne (named AM12-S31)was obtained. In this study, we investigated its drug resistant characteristics, viral titer and for murine hematopoietic progenitor cells transduction as well. MTT assay verified that the AM12-S31 cells were resistant to G418 and methotrexate(MTX), the IC50 were more than 800 ?g/ml and 100 ?M respectively while the control cell line AM12 was sensitive to both drugs, the IC50 were 180 ?g/ml and 10 ?M, respectively. The viral titer for this cell line was approximately 7.8? 104~4.2? 105 G418-resistant colony forming units/ml. The replication-competent virus can not be detected in this producer cell line. We also use the AM12-S31 cells to transfect murine hematopoietic cells (By coculture) . The positive colonies were found in all the G418 concentrations using CFU-GM assay. No G418-resistant colony was found using AM12 transfection. The infected murine marrow cells were returned to lethally irradiated(900rad)recipients. The murine transplanted with AM12-S31 infected marrow cells showed protection from lethal MTX toxicity as compared with AM12 infected animals. Evidence for integration and the proviral DNA was obtained by PCR amplification of proviral DNA. These results indicated this producer cell hne could produce high titer, high-efficiency and non-replcational competent virus. The murine marrow cells could be transfected successfully using this system, and express the foreign gene. The lethal irradiated murine marrow function could be reinstitution by infusing the hematopoietic progenitor cells tranducted with human mutant dihydrofolate reductase. In my opinion, this system would play an important role in research the long-term protection of murine marrow hematopoietic function and drug resistant gene therapy.
7.Dynamic expression of AQP4 in early stageof ischemia/reperfusion rats and cerebral edema
Shuhong XU ; Chen KANG ; Meiling CHEN ; Peipei ZHOU ; Guangwei HE ; Yajiao CUI ; Di YANG ; Yulin WU
Chinese Pharmacological Bulletin 2016;32(10):1433-1441
Aim To make a research of rats with focal cerebral ischemia/reperfusion injury on pathological changes in brain and the changes of AQP4 and related proteins, in order to explore the relationship between AQP4 and brain edema. Methods Adult male SD rats, weighting 250~300 g, were randomly divided in-to Sham group and cerebral ischemia/reperfusion ( I/R) injury model group. The I/R model group was di-vided into the I/R-6 h, 12 h, 24 h, 48 h-four time point groups. The animal model of the right MCA is-chemia/reperfusion was established by suture method in mature SD rats. The nerve symptom score was con-ducted in the corresponding time points. Then, the permeability of brain tissue was detected by EB stai-ning;TTC staining was conducted to observe the cere-bral infarction volume;the dry wet weight method was used to detect the changes of brain water content; im-munohistochemical( IHC) , WB and RT-PCR were ap-plied to detect the expression of AQP4 , and the related factors at different time points of the model rats after is-chemia-reperfusion around infarcts. Results Com-pared with the Sham group, then ever function score of the rats in I/R model groups were much higher. With the increase of the reperfusion time, the cerebral in-farction volume, brain tissue permeability and the brain water content were also increased. IHC results showed that AQP4 expression gradually rose with widen distribution. WB and RT-PCR results verified the in-creasing level of AQP4 expression. The detection of the related proteins expression showed apparent changes. The expression of MMP-9 was increased, while the Oc-cludin and JAM-1 expression showed a decreasing trend. The I/R-48 h model group showed the most ob-vious differences in the expression of the related pro-teins and mRNA ( P <0. 01 vs Sham, respectively ) . Conclusion Accompanied with the aggravating cere-bral injury after cerebral ischemia/reperfusion, the ex-pression of AQP4 and MMP-9 level were activated, while the degradation of TJPs, Occludin and JAM-1, was increased. These factors are combined to make the formation of brain edema. This study makes a further research on the formation mechanism of the early stage for cerebral edema on I/R model and offers a potential for intervention in the filed of looking for a reliable drug therapy on cerebral edema.
8.Structural modeling and dynamic evolution analysis of hypoxia response networks
Jianjie LI ; Jianwei MA ; Xuan YU ; Guangwei CHEN ; Li XU ; Zhaohui HUANG ; Yuqi GAO
Military Medical Sciences 2016;40(7):597-601
Objective To construct an executable model of a hypoxia response network (HRN) and to analyze the dynamic evolution mechanism of an HRN including randomness as well as concurrency based on computer simulation. Methods Specific evolution rules and Gillespie algorithm were adoped to study the dynamic evolution of the structural model based on the construction of a structural model of an HRN using stochastic Petri net ( SPN ) .Results Dynamic evolution laws of an HRN were obtained and the simulation results were consistent with laboratory results in response to descript switch-like behavior of an HRN .Conclusion A visualization model of the HRN can be achieved using SPN method.Simulation results achieved by executing the model based on stochastic simulation using specific kinetic parameters can serve as a nice complement to traditional laboratory results , which can help shed light on the structure and function characteristics of an HRN.
9.Modeling and analysis of triage in disaster rescue action executed by mobile medical unit using stochastic Petri net
Jianjie LI ; Jianwei MA ; Xuan YU ; Guangwei CHEN ; Li XU ; Zhaohui HUANG ; Yuqi GAO
Military Medical Sciences 2016;40(2):106-109,132
Objective To analyze the process of triage in disaster rescue action performed by a mobile medical unit so that the rescue process can be improved , the efficiency of rescue enhanced , and the decision on health service in rescue action is supported.Methods The process of triage in disaster rescue action was modeled based on stochastic Petri net while the performance of the model was analyzed quantitatively .Results and Conclusion The critical factor which affects the efficiency of rescue work is obtained by analyzing the performance of the model .
10.The analysis of multi-drug resistance of doxorubicin with flow cytometry.
Guoqiang ZHENG ; Xuyi LIU ; Fusheng HAN ; Guangwei XU
Chinese Journal of Lung Cancer 2002;5(1):28-30
BACKGROUNDTo evaluate the significance of flow cytometry (FCM) in the analysis of multi-drug resistance of doxorubicin.
METHODSThe level of doxorubicin or Rh-123 in S-180R and BGC-823/DOX, which were two cell lines with different drug resistance, was measured by FCM comparatively and continuously.
RESULTSThe total fluorescence profile peaks of S-180R, a high resistant cell line, were predominantly different from those of S-180, the parent cell line, by the FCM analysis. The subtle fluorescence profile differences between BGC-823/DOX (a low resistant cell line) and the parent cell line were quantitatively measured on the FCM map. The changes of each resistant cell fluorescence from the S 180R cells could be displayed by continuous tests, the more near the fluorescence level of each cell was, the more the fluorescence pike of whole cells centralized.
CONCLUSIONSFCM is a sensitive, accurate and quantitative test in the analysis of doxorubicin drug resistance.