In the pathology teaching process,teachers can help students comprehend the essential knowledge of pathology by means of dialectical thinking.The dialectical thinking method not only can make the students memorize the information better,but also can develop the correct thinking method of students and improve their capability of analyzing and solving problems.

Objective To investigate the clinical pathological feature and treatment of cystosarcoma phyllodesand.Methods 12 cases of cystosarcoma phyllodesand after surgical were retrospectively reviewed.Results Among the 12 cases cystosarcoma phyllodesand,5 cases underwent total mastectomy,2 cases underwent modified radical mastectomy,1 case underwent local excision and reserve nipple,and 4 cases underwent lesion removed and reserve nipple or gland.All cases were follow up between 1～18 years,1 case lost,2 cases recurred after surgical 1.5 and 2.0 years and cystosarcoma were removed again,and other patients were free from cancer cell.Conclusion The diagnosis and treatment of the cystosarcoma phyllodesand are based on the pathology.It is difficult to predict the behavior by the pure histology from the cell appearance,recurrence of the cystosarcoma phyllodesand is relevant with the different classification tumor and size.

OBJECTIVE: To investigate the clinical application of tissue engineering for treating tendon injury.METHODS: A computer-based online search was conducted in Medline and China Academic Journals Database with the key words of "Biological material, Sinew mechanism heals" in both English and Chinese from 1974 to 2009. Relevant books were hand-retrieved.RESULTS: Scholars at home and abroad tried to investigate how to regulate cell proliferation, differentiation, and matrix synthesis by endogenous and exogenous growth factors so as to promote tendon repairing, relieve tendon adhesion, and decrease attenuation of biomechanical intensity. Tendon detect was mainly treated by autologous tendon transplantation,allogeneic tendon transplantation, artificial tendon transplantation, and tissue-engineered tendon transplantation. All those methods had both advantages and disadvantages. However, artificial biomaterial tendon has attracted much attention and it has been used for clinical application. Of course, there are still more problems to be solved, for example, source of tissue-engineered seed cells, immunological reaction of allogeneic tendon cells, specific mechanical intension, preparation and selection of degradable scaffold materials, correlation between cell and scaffold materials, and correlation between cell-material compound and peripheral tissues. With the development of tendon repairing and healing, single drug which was used to prevent tendon adhesion has developed into drug-barrier compound. Additionally, with the development of Chinese herb and molecular biological products, they not only inhibited exogenous healing, but also promote endogenous healing. Meanwhile, simple surgery has also developed into combined therapy.CONCLUSION: Although biomaterials for tendon repairing have achieved breakthrough in some respects, the results need also to be further studied.

Objective To investigate the changes in biologic behavior of dendritic cells (DCs) after being modified by human telomerase reverse transcriptase (hTERT) gene. Methods In order to obtain the hTERT gene modified DCs, DCs were transfected with a replication-deficient recombinant adenovirus expression vector of hTERT. Then the expression to hTERT and PCNA was assessed by by Western blot, mature markers on DCs surface were detected by flow cytometry, and the proliferative capacity was determined by MTT method. Results Immunohistochemical staining and Western blotting showed that the expression of hTERT was upregulated obviously in DCs after being modified by hTERT gene. Flow cytometry indicated that the expression of CD83 and CD86 remained unchanged. The DC growth curve showed that the number of DC-hTERT was increased slightly in the first 2 weeks, meanwhile the number of DC/rAd-LacZ and DC was decreased obviously. Although the number in 3 groups was all decreased in the third week, the number of DC/rAd-hTERT was still greater than the other control groups. It was also found that the expression of PCNA was increased in DC/rAd-hTERT compared with that of immature DC, mature DC or DC/rAd-LacZ by Western blot. Conclusion After rAd-hTERT modification, life span of DC in vitro is extended and proliferative capacity is enhanced.

0 5).Conclusions The measurement of platelet membrane GPⅡbⅢa complexes by FCM can provide a simple, sensitive and reliable method for G T diagnosis,and also can be applied to analysis of GT family pedigree.

Objectives To establish culture system for unilineage differentiation of umbilical cord blood CD 34 + cells into granulocytes, erythrocytes or platelets. Methods After CD 34 + cells were separated by midiMACS using micro beads conjugated with anti-CD 34 monoclonal antibody, these cells were induced to specifically differentiate along granulocyte, erythrocyte or platelet by adding appropriate hematopoietic growth factors including SCF plus G-CSF, EPO or TPO. Then morphology, immunological phenotype and function analysis were performed to identify these induced cells. Results After induced unilineage differentiation, the percentages of CD 15 +, GPA + and CD 41 + cells were 81 17%, 95 35% and 77 82%, respectively. These induced cells acquired terminal cell's morphologies. The cells from the granulocytic culture had the ability to phagocytose Chinese ink and plate-like particles could aggregate under the action of thrombin. Conclusion The culture system for unilineage differentiation of CD 34 + cells into erythrocytes, granulocytes, or platelets was established, which was a basis for the research about cell treatment, gene regulation and exogenous gene expression in hemopoiesis.

Objective To evaluate the practical efficacy of comparison method in experimental teaching of pathological anatomy.Methods The junior college students of stomatology were divided into experimental group (n =48) and control group (n =48) randomly.Students in experimental group adopted the experimental teaching with comparison method of pathological anatomy and histology while those in control group followed the traditional experimental teaching.Experiment examination results of two groups were compared and questionnaire investigation was carried out among students in experimental group.Results Average score of experiment examination in experimental group was (88.35 ±5.68) while that in control group was (78.35 ±4.86),with statistical differences (P ＜0.01).The results of questionnaire investigation showed that students in experiment group preferred comparison teaching method.Conclusions Comparison teaching method is feasible,the mechanism of which emphasizes gaining new knowledge by reviewing the old and strengthens the contact of basic subjects,therefore it is conducive to enhancing the quality of pathological experimental teaching.

Objective To evaluate influence of general anesthesia on postoperative cognition function in the elderly patients by using different methods and choose a better anesthesia method for elderly patients. Methods Forty elderly patients undergoing selective abdominal surgery were selected and divided into two groups: anesthesia was maintained with general anesthesia combined with epidural anesthesia in observe group and with general anesthesia singlely in control group. Vital signs were observed and recorded during operation and blood pressure varied within the extent of 20% of basic level. Cognition function of each patient 24 hours before and 24,48 hours after operation was e-valuated by MMSE method. Results All the patients maintained steady vital signs and there was no significant differ-ence in MMSE scores between two groups at all the time points. There were 11 cases(55% ) who had acute cognitive dysfunction in observe group and 13 cases(65%) in control group 24h after operation(P>0.05) ,5 cases(25%) in observe group and 9 cases(45%) in control group 48h after surgery(P>0.05). Conclusion Compared with single general anesthesia, general anesthesia combined with epidural anesthesia uses less general anesthetics and has less negative effect on postoperative cognition funetion in the elderly patients,it maybe better in elderly patients undergoing non-cardiac operation.

BACKGROUND: There still is rarely report about the effect of glycosylphosphatidilinoditol-specific phospholipase D (GPI-PLD) on the adhesion function of leukemic cells through screening Medline and CNKI databases.OBJECTIVE: To observe the effect of GPI-PLD on the adhesion function of bone marrow mononuclear cells separated from myeloid leukemia patients, and to investigate the related mechanism. DESIGN, TIME AND SETTING: This study addressing cytology in vitro was conducted at the Hematological Laboratory of Xiangya Hospital from January to June 2004.MATERIALS: Bone marrow was collected from myeloid leukemia patients at the Department of Hematology, Xiangya Hospital, China.METHODS: The GPI-PLD activity of bone marrow mononuclear cells separated from myeloid leukemia patients was measured by using GPI-anchored placental alkaline phosphatase as substrate and Triton-X114 partition. By use of 1,10-phenanthroline, the activity of GPI-PLD was inhibited, the experiment was divided into 2 groups: treatment group adding phenanthroline to achieve a final concentration of 1 mmol/L, while control group adding the same amount of phosphate buffered saline. The adhesion rate to the fibronectin and CD24 expression of these cells were measured by MTT and immunohistochemical method, respectively.MAIN OUTCOME MEASURES: GPI-PLD activity of myeloid leukemic cells, cell adhesion rate, CD24 expression were all measured. RESULTS: The GPI-PLD activity of bone marrow mononuclear cells separated from myeloid leukemia patients was inhibited significantly after these cells were treated by 1 mmol/L 1,10-phenanthroline for 5 hours compared with control groups [(5.40±2.96)%, (42.08±7.21)%, P < 0.01]. At the same time, the adhesion rate of these cells were increased after the GPI-PLD activity was inhibited [(61.19±29.14)%, (49.78±26.73)%, P < 0.01], and the CD24 expression was also up-regulated [(18.5±11.14)%, (16.02±9.68), P < 0.01].CONCLUSION: The adhesion rate of bone marrow mononuclear cells separated from myeloid leukemia patients can be promoted by inhibiting GPI-PLD activity. At the same time, the CD24 expression of GPI-anchored proteins on bone marrow mononuclear cells is improved.

Objective To evaluate the prognostic value of white blood cell and hemoglobin in patients with acute aortic dissection(AAD).Methods White blood cell.hemoglobin and fibrinogen were measured in 42 AAD patients.The mortality during hospitalization was observed and the short-term prognosis in AAD patients was as-seased.Results There was 13 death during hospitalization.with the mortality of 30.95%.The value of white blood cell was higher in death group than in survival group[(13.73±6.91)×109>/L vs.(9.43±4.97)×109>/L.P< 0.05).The value of hemoglobin was lower in death group of than in survival group[(118.54±22.38)g/L vs. (131.72±18.17) g/L,P<0.05].There were no differences in the value of fibrinogen between the groups [(3.15±1.15)g/L vs.(3.48±1.24)g/L,P>0.05).The mortality in the group of elevated white blood cell(≥10.0×109>/L)was higher than that in the group of normal white blood cell(10.0×109/L)(41.18%vs.24%.P<0.05).The mortality in the group of decreased hemoglobin(≤110g/L)was higher than that in the group of nor-mal hemoglobin(>110 g/L)(50.00%vs.27.78%,P<0.05).Conclusion The value of white blood cell and he-moglobin could help to assess the short-term prognosis of patients with AAD.