1.Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal.
Mengyu GAO ; Xinglong ZHU ; Shisheng WANG ; Bingqi ZHANG ; Yunlin ZHANG ; Yuting HE ; Yanyan ZHOU ; Shun LI ; Guang YANG ; Guangneng LIAO ; Ji BAO ; Hong BU
Journal of Biomedical Engineering 2021;38(1):111-121
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
Animals
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CRISPR-Cas Systems/genetics*
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Clustered Regularly Interspaced Short Palindromic Repeats/genetics*
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Gene Editing
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RNA, Guide/genetics*
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Swine
Result Analysis
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