Objective To observe the number of pulmonary mast cell tryptase(MCT) after amniotic fluid embolism(AFE) in experimental rabbits.Method The experimental amniotic fluid embolism rabbits model was established by injecting the amniotic fluid(AF) or placental extracts mixed with amniotic fluid(PAF)into the marginal vein of the pregnant rabbits.Immunohistochemistry and microscopy were employed to observe the number of pulmonary MCT in AFE rabbits.Results A marked increase in MCT positive cells was observed in lungs in both AF and PAF groups(average cell number were 32 98 and 45 53respectively)as compared with the control group(average cell number was 11 25).Conclusion The results indicated that the number of MCT cells were marked increased in case of the AFE.

Objective To observe the dynamic changes in plasma coagulation indexes in the rabbit's amniotic fluid embolism. Method The experimental amniotic embolism model was established by injecting the amniotic fluid or placental extract-amniotic fluid through the rabbit's marginal vein of the ear. Blood samples were taken from the heart before and after infusion. Fibrinogen (FIB), prothrombin time (PT), thrombin time (TT), and activated partial thromboplastin time (APTT) were measured with coagulation analyzer. Results As compared with the sham operation group, FIB decrease significantly after infusion with amniotic fluid and placenta! extract mixed with amniotic fluid (P

Objective Carry out the mast cell tryptase (MCT) granule count in throat, lung and small intestine mast cells in persons died of anaphylactic shock.Method Control group: 10 cases died from severe brain injury. Research group:15 cases died from anaphylactic shock(group A)and 8 cases died of amniotic fluid embolism (group B). H & E staining and immunohistochemical staining were employed and the MCT granule count performed on the throat, lung and small intestine tissue.Results There was marked increase of MCT granule count in the throat (average MCT-positive number of group A 28.48 and of group B 24.35),lung (average MCT-positive number of group A 46.98 and of group B 43.50) and small intestine (average MCT-positive number of group A 48.23 and of group B 42.72) in specimens of group A and group B as compared with the control group (average number in throat 7.97,lung 12.94 and small intestine 20.25).Conclusion Tryptase granular count of MCT increased in throat, lung and small intestine tissue in persons died from anaphylactic shock and amniotic fluid embolism.

Objective To explore the pathognomonic findings of anaphylactic shock in forensic medicine. Methods Animal model of anaphylactic shock was developed in guinea pigs. Substance P level in plasma was determined radioimmunoassay, and detected in laryngopharyneal tissue and the lungs by immunohistochemical SABC method followed by image analysis using BI - 2000 image analyzing system. Positive index (PI) was calculated. Results There was a statistically significant difference for substance P concentration in plasma between the guinea pigs with anaphylactic shock (131.01? 18.93pg/?l)and the control animals (87.70 ?7. 60pg/?l) (P