1.Clinical observation on acupuncture combined with tuina manipulation for thoracic facet joint disorder
Guangming ZHOU ; Jun JIANG ; Yan ZHANG
Journal of Acupuncture and Tuina Science 2015;(4):246-250
Objective:To observe the clinical effect of acupuncture combined with tuina manipulation for thoracic facet joint disorder. Methods:A total of 93 eligible cases were randomly allocated into an observation group and a control group. Patients in the observation group (n=46) were treated with acupuncture combined with tuina manipulation, whereas patients in the control group (n=47) were treated with tuina manipulation alone. The treatment was done once a day, for a total of 3 times. Therapeutic efficacies were then evaluated according to scores on signs and symptoms.
Results:After treatment, there were intra-group statistically significant differences in scores of eight signs and symptoms (all P<0.01); and the between-group differences were statistically significant in scores of eight signs and symptoms (all P<0.05). After three times of treatment, the recovery rate in the observation group was 54.4%, versus 25.4% in the control group, showing a statistical significance (P<0.05).
Conclusion:Acupuncture combined with tuina manipulation can obtain better effects than tuina manipulation alone for thoracic facet joint disorder and is therefore worth further clinical application.
2.Chemical Reaction of VOCs in Indoor Air and Health Effects
Zhou ZHU ; Guangming ZENG ; Min XU
Journal of Environment and Health 1992;0(04):-
Many kinds of volatile organic compounds(VOCs)exist in the indoor air,the chemical reaction will occur among the pollutants when O3 and NO2 exist simultaneously.This reaction can severely impact the indoor air quality and produce some adverse effects on human health.The main chemical reactions among the indoor air pollutants and the potential health effects associated with inhalation exposure to the products of the chemical reaction in the indoor air were discussed in the present paper.The study of the chemical reactions among indoor VOCs is very important for human health and is helpful for revising the Indoor Air Quality Standard and making the 'Ecologic Label' of building materials.
3.HPLC Determination of Aloin and Aloeemodin in Barbodos Aloe
Guilong PENG ; Guangming ZHOU ; Hongying QIN
Chinese Journal of Information on Traditional Chinese Medicine 2013;(12):59-60,61
Objective To isolate and determine Aloin and Aloeemodin in Barbodos Aloe by ionic liquid-based ultrasonic-assisted extraction coupled with high performance liquid chromatography, with 1-butyl-3-methylimidazolium chloride ([BMIM]Br) solution as the extraction solvent. Methods The separation was performed on Phenomenex C18 column (250 mm×4.6 mm, 5 μm) with detection wavelength of 360 nm. The mobile phase was consisted of methanol-0.3% acetic acid solution (65∶35) with the flow rate of 0.80 mL/min, and the column temperature at 35 ℃. Results The calibration curves for Aloin and Aloeemodin were liner within 0.000 336-1.68 μg (r=0.999 96) and 0.000 608-3.04 μg (r=0.999 76), respectively. The limit of detection (LOD) was 0.05 06 ng/mL and 0.262 ng/mL, respectively. The average recovery was 95.99% and 95.80%, respectively. Conclusion The method is simple, rapid, accurate, sensitive, low cost and environment-friendly, thus it provides an effective means for assaying anthraquinones in Barbodos Aloe.
4.Effect of Ammoniummeta Vanadate on Tissue Structure of Jejunum in Rats
Jinxing ZHOU ; Jue WANG ; Guangming JIN
Journal of Environment and Health 1992;0(04):-
Objective To study the influence of ammoniummeta vanadate on the histological structure of jejunum of rats. Methods One hundred and twelve healthy and clean SD rats were randomly divided into the high dosage (60 mg/L),the moderate dosage(40 mg/L),the low dosage(20 mg/L) and the control group(distilled water),28 in each group,the males and females in the same number,the administration was conducted through drinking water,for 8 consecutive weeks. In the 4th and 8th weeks,the length of jejunum villus,the depth of jejunum gland and the thickness of jejunum wall were measured,the ratio of villus length and gland depth were counted and the pathological examination was done. Results With the increase of ammoniummeta vanadate,the weight of SD rats,the jejunum villus length,the depth of the jejunum gland,the wall thickness had showed a downward trend,the ratio of villus length and gland depth were significantly lower (P
5.Determination of Puerarin, Daidzin and Daidzein in Puerariae Lobatae Radix by ILDLPME Coupled with HPLC
Guilong PENG ; Guangming ZHOU ; Hongying QIN ; Junhua CHEN
Chinese Journal of Information on Traditional Chinese Medicine 2014;(10):67-70
Objective To develop a sensitive and specific HPLC method to simultaneously determine the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix. Methods The three compounds were obtained by ionic liquid based on dispersive liquid phase microextraction. The determination was carried out on a Phenomenex C18 column (250 mm×4.6 mm, 5μm) with a mixture of methanol-0.2%acetic acid (volume ration 45∶55) as mobile phase at a flow rate of 0.8 mL/min. The UV detective wavelength was 250 nm, and the column temperature was set at 35 ℃. Results The linear response ranged from 6.24×10-6-37.44 μg for puerarin (r=0.999 71), 5.44×10-6-27.20μg for daidzin (r=0.999 85), and 5.60×10-6-28.00μg for daidzein (r=0.999 94), respectively. Conclusion The method is quick, simple and repeatable for simultaneous determination of the contents of puerarin, daidzin and daidzein in Puerariae Lobatae Radix.
6.Total proctocolectomy with SMA & V severance and ileal pouch-anal anastomosis for familial polyposis coli
Jiazhen ZHOU ; Guangming LI ; Tao LIU ; Mingyang REN ; Chenggang WEI
Chinese Journal of General Surgery 2009;24(2):119-121
Objective To investigate the clinical value of a newly designed surgical therapy for familial polyposis coli by severing the superior mesenteric artery&vein in order to make a complete lysis of the mesentery and an ileum pouch and the anal anastomosis within the entire muscular sheath of the rectum.Methods Six patients with familial polyposis coli(5 males and 1 female,aged 24-36 years)were admitted and underwent the procedure which was consisted of:(1)An incision was made in the left middle and lower parts of the rectus abdominis;(2)The greater omentum was retained and the large intestine was removed;(3)At the juncture of the sigmoid colon and the rectum,the muscular sheath was dissociated 0.5cm,the mucous membrane of the rectum was stripped in a revolving manner,the nourishing artery and vein in the membrane were exposed,and clamped and cut in sequence up to the anocutaneoue line;(4)The rectal mucous membrane was completely removed;(5)Under the right colonic artery,the superior mesenteric artery and vein were severed;(6)An N-,J-or W-shaped pouch was made in the ileum accordingly:(7)An anastomosis of the ileum pouch and the anal canal was made within the entire muscular sheath of the rectum,and a drainage was placed;(8)The mesostenium was fixed on the fight posterior abdomen,the small intestines were spread out to the right side,and the mesostenium was covered on the coarse surface of the colon bed:(9)A tube was placed in the left lower abdomen for a vacuum aspiration for 2 days after operation,combined with the suction drainage,to eliminate the pelvic effusions;and(10)The abdomen was closed.Results Patients were able to discriminate stools and flatus 3-7 days after operation.and the formed stools occurred 7-10 days after operation.Five patients were followed-up for 3-17 years,with averagely one defecation a day,with no night defecation and seepage.Urination was normal;In another one patient who underwent the procedure 4 months ago the defecation was twice a day,with no night defecation.All the 6 patients had normal autonomic nerve function and sexual function as well as normal defecation and urination,with no recurrence of polyposis coli or infection.The small bowel functions well with no ischemia related symptoms.Conclusion Cutting the superior mesenteric artery and vein and then making anastomosis of the ileum pouch and the anal canal within the muscular sheath of the rectum is a new surgical approach to familial polyposis coli.It is safe and significantly improves the patients' life quality.
7.A mandibular canine with two roots and two root canals: A case report
Guangming LUO ; Yunfeng YANG ; Yu JIANG ; Li ZHOU
Journal of Practical Stomatology 2009;25(4):597-598
The mandibular canine usually has a single-root with a single canal. A case of a patient who has a mandibular canine with two well-defined roots and two root canals was reported. Size of the lingual root and buccal root was equal. The buccal root length was 24.5 mm. The lingual root length was 23.0 mm. Clinicians should be aware of the anatomical variation that exists in mandibular canines in practice.
8.The value of dual-energy CT in diagnosing pulmonary embolism in children
Chunxiang TANG ; Song LUO ; Longjiang ZHANG ; Changsheng ZHOU ; Guangming LU
Chinese Journal of Radiology 2013;47(10):912-916
Objective To evaluate the value of dual energy CT with Lung Vessels application in diagnosing pulmonary embolism (PE) of children.Methods Forty-seven patients with nephrotic syndrome and aged ≤ 18 years old (32 male,15 female,mean age 15 years) underwent dual-energy CT pulmonary angiography (CTPA).Two radiologists recorded the presence or absence of emboli and counted the clots on a per-patient and per-lobe basis with Lung Vessels,respectively.With conventional CTPA as reference standard,sensitivity,specificity,positive predictive value (PPV),negative predictive value (NPV) of Lung Vessels application to detect PE were computed for two readers.Inter-reader agreement was also calculated.Results Conventional CTPA detected 51 clots in 35 lung lobes in 13 patients,giving PE incidence was 27.6% (13/47) for this cohort.On a per-patient basis,readers 1 and 2 detected 25 and 21 patients with PE with Lung Vessels,corresponding to sensitivities,specificities,PPVs,and NPVs of 100.0% (13/13),58.6% (17/29),43.3% (13/30),100.0% (17/17) and 100.0% (13/13),76.5% (21/29),50.0% (13/26),100.0% (21/21),respectively.On a per-lung lobe basis,readers 1 and 2 detected 74 and 63 clots with Lung Vessels,respectively,corresponding to sensitivities,specificities,PPVs,and NPVs of 94.3% (33/35),79.5% (159/200),44.6% (33/74),98.7% (159/161) and 97.1% (34/36),85.5% (171/200),54.0% (34/63),98.8% (171/173),respectively.On a per-clot basis,134 and 105 clots were detected with Lung Vessels application,corresponding to sensitivities,specificities,PPVs,and NPVs for DECT of 96.1% (49/51),64.9% (159/245),36.3% (49/135),98.7% (159/161) and94.1% (48/51),75.0% (171/228),45.7% (48/105),98.3% (171/174)for readers 1 and 2,respectively.Excellent or good inter-reader agreement was observed on a per-patient,per-lobe and per-clot basis (Kappa =0.828,0.859,0.695 ; all P < 0.01).Conclusions Dual-energyCT with Lung Vessels application has a high sensitivity and negative predictive value for PE detection in children.More clots are detected with Lung Vessels application than conventional CTPA,which deserve further investigation.
9.Influence of psychological nursing on the therapeutic effect of patients with newly diagnosed type 2 diabetes
Qinghua FU ; Huifang YE ; Ke ZHOU ; Guiying MO ; Guangming LAN
Chinese Journal of Practical Nursing 2008;24(18):55-56
Objective To discuss the influence of psychological nursing on the therapeutic effect of patients with newly diagnosed type 2 diabetes. Methods Patients (60 cases) who received psychological nursing were set as the nursing group. Patients (65 cases) who did not received psychological nursing were set as the control group. The number of patients who received early insulin treatment and whose fasting plasma glucose return to normal value on the 7th and 30th days after treatment was observed. Results The number of patients who received early insulin treatment and whose fasting plasma glucose return to normal value on the 7th and 30th days after treatment in the nursing group was higher than that of the control group (P<0.01). Conclusions Psychological nursing of patients with newly diagnosed type 2 diabetes could make patients obey doctors' advice and accept early insulin treatment to control plasma glucose and delete the toxicity of high concentration of plasma glucose as soon as possible.
10.Hemangioblastoma: clinicopathologic analyses of 40 cases
Chunhui ZHOU ; Zhuofang HAO ; Degui LIAO ; Guangming LU ; Shizhang HUANG
Chinese Journal of Clinical and Experimental Pathology 2010;(1):60-62,66
Purpose To analyze the clinicopathologic features, immunophenotypes, image features and diagnosis of hemangioblastoma.Methods The clinical and pathological features were studied with HE and immunohistochemical staining in 40 cases of hemangioblastoma.The image features were studied with CT and MRI.Results The clinical symptoms of these cases were dizziness,headache,vomitting, optic disc edema and ataxia. The CT and MRI showed a sharply demarcated tumor with cystic areas and a solid mural nodule. After enhancement scanning, the mural nodule was usually enhanced and the wall of the cystic area was not. Histopathologically, this tumor was characterized by two main components: capillary and stromal cells. Immunohistochemically, the endotheliocyte was positive for CD34 and FⅧRAg, but most of the stromal cells were positive for S-100 and part of the cells were also positive for NSE. The endotheliocyte and the stromal cell were all positive for vimentin, but negative for GFAP, EMA and p53. The expression of Ki-67 was very low.Conclusions Hemangioblastoma is characterized by stromal cells and numerous capillary, but the origin of the stromal cell is not clear. Its image features have some characteristics. It needs to be distinguished from pilocytic astrocytoma, angiomatous meningioma and renal carcinoma.