Objective To establish the quality standard for Jianpi Fugan Pills. Methods Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome in the formulation were qualitatively identified by TLC. The content of ferulic acid was analyzed by HPLC. TIANHE C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.085% phosphorous acid (17∶83). The content was detected at wavelength of 323 nm. The flow rate was 1.0 mL/min, and temperature was 25 ℃. Results The spots in the TLC of Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome were in the same chormatograms of control articles. The linear range of ferulic acid was 0.114-1.482 μg. The average recovery was 98.36%and RSD was 1.16%. Conclusion The method is simple, accurate and highly sensitive, which can be used for quality control of Jianpi Fugan Pills.

Objective To introduce the role of pancreatic stellate cells in pancreatic fibrosis and the progress in treatment of pancreatic fibrosis. Methods Relevant literatures were collected and reviewed. Results Pancreatic stellate cells activation was closely related to pancreatic fibrosis. Inhibition of pancreatic stellate cells activation could provide a new approach in clinical treatment of chronic pancreatitis. Conclusion Pancreatic stellate cells are the key to pancreatic fibrosis,which are becoming the target for anti-fibrosis of the pancreas and treatment of chronic pancreatitis.

lymorphism of CD14 gene is a risk factor for diabetic nephropathy.

BACKGROUND:Cholesterol is closely linked to the occurrence and progression of atherosclerosis. Current approaches to study celular cholesterol dynamics have their own limitations.
OBJECTIVE: To measure the cholesterol efflux rate of RAW 264.7 mouse macrophages by BODIPY-Cholesterol labeling and to explore the effects of extracelular cholesterol and lipopolysaccharide on the cholesterol efflux rate.
METHODS:RAW 264.7 cels were cultured in vitro with DMEM containing 10% fetal bovine serum, and labeled with BODIPY-Cholesterol for 1, 2, 4, 8 hours. Then, the cels were rinsed with serum-free DMEM and inoculated for 6, 12, 24, 48, 96 hours to optimize the labeling time and incubation time. We measured and compared the cholesterol efflux rates after cultured cels were treated with cholesterol, lipopolysaccharide, human sera with high cholesterol or human sera with normal cholesterol.
RESULTS AND CONCLUSION: The best labeling time for BODIPY-Cholesterol was 2-8 hours. Cholesterol efflux rates were gradualy decreased after the cels that were labeled for 2 hours were incubated with increasing concentrations of cholesterol (0.1, 0.5, 2.5 mmol/L,P< 0.01). Treating cels with lipopolysaccharide also decreased the cholesterol efflux rate (P < 0.05). Furthermore, the cholesterol efflux rate was decreased after cels were treated with human sera with high cholesterol (P< 0.05). These findings indicate that BODIPY-Cholesterol can be used to measure celular cholesterol efflux rate and to study the effects of extracelular cholesterol and lipopolysaccharide on the cholesterol efflux rate.

Objective To investigate the characteristics of memory impairment in patients with ischemic cerebrovascular diseases(ICVD)for further understanding in the cognitive impairment at early stage.Methods Forty-six ICVD patients aged 49 to 80 years old and forty-six matched volunteers,all from the same hospital during Sep 2005 to Feb 2006,were enrolled.Mini-mental state examination(MMSE),clinical memory scale(CMS),Fuld object-memory evaluation(FOM)and rapid verbal retrieve(RVR)were used to evaluate the cognitive and memory function.Results The ICVD patients had lower scores in every memory function than the controls(P

In our laboratory, injection of HGF, prepared by the modified Labrecque's method, was used for the treatment of experimental hepatic injury in rats. It was found that the serum estradiol level was significantly higher in the group of HGF treatment than that in the control group (P

The majority of patients with malignant tumors have hypercoagulable state,which easily leads to thrombosis,and is closely related with recurrence of tumor metastasis.The formation mechanism of hypercoagulable state is related to tumor,tumor treatment,complications etc.The main diagnostic indicators are the platelet,cruor and fibrinolysis,P-selectin and lysosomal protein,blood rheology.Tumor patients with the high risk of thrombus should use low molecular weight heparin.Thromboembolism should be prevented in bedridden patients with tumor and tumor associated operation.Tumor patients with venous thromboembolism should be given thrombolytic treatment.Tumor patients with hypercoagulable state should be treated by antico-agulant therapy combined with chemotherapy.

Granger causality model is an analysis method that requires no priori knowledge and emphasizes time sequence. Such model applied to brain effective connectivity network can reflect the directional connectivity among brain regions or neurons. This paper reviews the principle of Granger causality model, basic test steps and improved models, analyzes and discusses applications and existing problems of Granger causality model in brain effective connectivity network.
Brain ; physiology ; Brain Mapping ; Computer Simulation ; Humans ; Models, Neurological ; Neurons ; physiology

Objective To evaluate monoenergetic imaging of dual energy CT in the visualization of metal fixation of factures. Methods In total, 29 patients with factures underwent 36 metal fixations,including 11 external fixations implanting in tibiofibula (n = 11 ) and 25 internal fixations (cervical spine,n=10; lumbar spine, n=4; tibiofibula, n=8; radial bone, n=3). They were recruited into this study.After dual energy CT scan, monoenergetic software was used to post-process with the following 6 photon energies: 40, 70, 100, 130, 160, 190 keV. Two radiologists evaluated and rated the reformatted images with 6 different photon energies and average weighted 120 kV images according to the following 4-score scale. Score 1: nonassessable, with marked artifact; score 2: assessable, moderate artifact; score 3: good,mild artifact, good visualization of bony structures; score 4: excellent, no artifact. Kruskal-Wallis was used to perform statistical analysis of image quality for total fixations, external and internal fixations with various selective kev settings and average weighted 120 kV. Results For total fixations, monoenergetic imaging of dual energy CT has 25 fixations with score 3 and 4, but 120 kV has only 4 fixations with score 3 and 4.Monoenergetic imaging of dual energy CT improved image quality of fractures with metal fixation compared to average weighted 120 kV images ( F = 116. 487, P ＜0. 01 ). Images of 130 kev had the best image quality for external metal fixation (9 fixations with score 3 and 4, F = 60. 902, P ＜ 0. 01), while 70 kev was best for internal metal fixation ( F = 122. 149, P ＜ 0. 01). Conclusions Monoenergetic imaging of dual energy CT improves image quality of fractures with metal fixation. Reformatted images at 70 keV and 130 keV havethe optimal image quality for internal and external metal fixation, respectively.

The successful cryopreservation of reproductive cells has important practical significance in many fields. In order to improve the recovery rate and viability of cryopreserved cells, it is necessary to study the permeability characteristics of cell membrane to both water and cryoprotectant. In this paper we review the studies on membrane permeability of animal reproductive cell for the recent years. We firstly list the typical permeability data of spermatozoa and oocyte membrane for water and cryoprotectant. We then analyze the effects of these characteristics on the design of cryopreservation protocol. We also introduce the latest experimental methods to measure the cell membrane permeability.
Animals ; Cell Membrane Permeability ; physiology ; Cryopreservation ; methods ; Female ; Humans ; Male ; Oocytes ; cytology ; Spermatozoa ; cytology