Objective To establish the quality standard for Jianpi Fugan Pills. Methods Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome in the formulation were qualitatively identified by TLC. The content of ferulic acid was analyzed by HPLC. TIANHE C18 column (250 mm×4.6 mm, 5 μm) was used with a mobile phase of acetonitrile-0.085% phosphorous acid (17∶83). The content was detected at wavelength of 323 nm. The flow rate was 1.0 mL/min, and temperature was 25 ℃. Results The spots in the TLC of Angelicae Sinenis Radix and Atractylodis Macrocephalae Rhizome were in the same chormatograms of control articles. The linear range of ferulic acid was 0.114-1.482 μg. The average recovery was 98.36%and RSD was 1.16%. Conclusion The method is simple, accurate and highly sensitive, which can be used for quality control of Jianpi Fugan Pills.

Objective To introduce the role of pancreatic stellate cells in pancreatic fibrosis and the progress in treatment of pancreatic fibrosis. Methods Relevant literatures were collected and reviewed. Results Pancreatic stellate cells activation was closely related to pancreatic fibrosis. Inhibition of pancreatic stellate cells activation could provide a new approach in clinical treatment of chronic pancreatitis. Conclusion Pancreatic stellate cells are the key to pancreatic fibrosis,which are becoming the target for anti-fibrosis of the pancreas and treatment of chronic pancreatitis.

lymorphism of CD14 gene is a risk factor for diabetic nephropathy.

The majority of patients with malignant tumors have hypercoagulable state,which easily leads to thrombosis,and is closely related with recurrence of tumor metastasis.The formation mechanism of hypercoagulable state is related to tumor,tumor treatment,complications etc.The main diagnostic indicators are the platelet,cruor and fibrinolysis,P-selectin and lysosomal protein,blood rheology.Tumor patients with the high risk of thrombus should use low molecular weight heparin.Thromboembolism should be prevented in bedridden patients with tumor and tumor associated operation.Tumor patients with venous thromboembolism should be given thrombolytic treatment.Tumor patients with hypercoagulable state should be treated by antico-agulant therapy combined with chemotherapy.

BACKGROUND:Cholesterol is closely linked to the occurrence and progression of atherosclerosis. Current approaches to study celular cholesterol dynamics have their own limitations.
OBJECTIVE: To measure the cholesterol efflux rate of RAW 264.7 mouse macrophages by BODIPY-Cholesterol labeling and to explore the effects of extracelular cholesterol and lipopolysaccharide on the cholesterol efflux rate.
METHODS:RAW 264.7 cels were cultured in vitro with DMEM containing 10% fetal bovine serum, and labeled with BODIPY-Cholesterol for 1, 2, 4, 8 hours. Then, the cels were rinsed with serum-free DMEM and inoculated for 6, 12, 24, 48, 96 hours to optimize the labeling time and incubation time. We measured and compared the cholesterol efflux rates after cultured cels were treated with cholesterol, lipopolysaccharide, human sera with high cholesterol or human sera with normal cholesterol.
RESULTS AND CONCLUSION: The best labeling time for BODIPY-Cholesterol was 2-8 hours. Cholesterol efflux rates were gradualy decreased after the cels that were labeled for 2 hours were incubated with increasing concentrations of cholesterol (0.1, 0.5, 2.5 mmol/L,P< 0.01). Treating cels with lipopolysaccharide also decreased the cholesterol efflux rate (P < 0.05). Furthermore, the cholesterol efflux rate was decreased after cels were treated with human sera with high cholesterol (P< 0.05). These findings indicate that BODIPY-Cholesterol can be used to measure celular cholesterol efflux rate and to study the effects of extracelular cholesterol and lipopolysaccharide on the cholesterol efflux rate.

In our laboratory, injection of HGF, prepared by the modified Labrecque's method, was used for the treatment of experimental hepatic injury in rats. It was found that the serum estradiol level was significantly higher in the group of HGF treatment than that in the control group (P

Objective To investigate the characteristics of memory impairment in patients with ischemic cerebrovascular diseases(ICVD)for further understanding in the cognitive impairment at early stage.Methods Forty-six ICVD patients aged 49 to 80 years old and forty-six matched volunteers,all from the same hospital during Sep 2005 to Feb 2006,were enrolled.Mini-mental state examination(MMSE),clinical memory scale(CMS),Fuld object-memory evaluation(FOM)and rapid verbal retrieve(RVR)were used to evaluate the cognitive and memory function.Results The ICVD patients had lower scores in every memory function than the controls(P

Objective At present, there is no study on effect of levetiracetam(LEV) on the gray matter structure remodeling in benign epilepsy children with central temporal spikes(BECTS).The purpose of this study was to study the influence of LEV on the gray matter structure in BECTS and to evaluate the mechanism of LEV on the brain structure of BECTS through using voxel-based MRI morphological(VBM) methods.Methods From January 2014 to September 2016, twenty-four BECTS treated with LEV(LEV group), twenty-four drug-na?ve BECTS(untreated group) and twenty-four normal children(normal group) consulted in department of Neurology, Nanjing Children′s Hospital and the Nanjing Military Region, Nanjing General Hospital were continuously included to receive three-dimensional T1-weighted imaging with 3T MRI and the gray matter volume was calculated by VBM.We compared the difference of grey matter volumes of the three groups and analyzed their correlation with epilepsy duration, age of onset and medication time and other clinical index.Results Compared with the normal group, the grey matter volume of bilateral thalamus were decreased, and the volume of bilateral Rolandic areas, anterior insula/frontal operculum/frontal triangle, left supplementary motor area, paracentral lobule, precentral gyrus, superior frontal gyrus and right middle frontal gyrus were increased in the untreated group, but the grey matter volume of the bilateral Rolandic areas, frontal operculum and left supplementary motor area were decreased in the LEV group.Compared with the untreated group, the grey matter volume of bilateral supplementary motor, left paracentral lobule, precentral gyrus, bilateral anterior insula/frontal operculum/frontal triangle, left superior frontal gyrus and right middle frontal gyrus in the LEV group were decreased.The grey matter volume of left anterior insula/frontal operculum areas was negatively correlated with the medication time in LEV group(r=-0.527, P<0.01).Conclusion T The mainly representations of BECTS are thalamic gray matter damage and epileptic-related cortical area irritation structural abnormalities, but the LEV could reshape the epilepsy-related cortical area and the gray matter in the brain area associated with clinical symptoms.

[ ABSTRACT] AIM:To investigate the mechanism of L-type calcium channel ( L-Ca2+)/calpain signal transduc-tion pathway in verapamil inversing resistance of papillary thyroid carcinoma to doxorubicin .METHODS:Human papillary thyroid carcinoma TPC-1 cells were cultured for 2 d.For determining the appropriate concentrations and treatment time of verapamil and doxorubicin , a compatibility test was conducted to detect the cell viability by CCK-8 assay.The cells were divided into control group , doxorubicin group , verapamil group and doxorubicin +verapamil group .The techniques of whole-cell patch-clamp was used to record L-Ca2+currents.The protein expression levels of calpain 1 and LC3 were detec-ted by Western blot .RESULTS: Compared with control group , the density of L-Ca2+current decreased in doxorubicin group and verapamil group (P<0.05).Compared with verapamil group , the density of L-Ca2+current decreased in doxo-rubicin+verapamil group (P<0.01).Compared with control group, the expression of calpain 1 decreased in doxorubicin group and verapamil group (P<0.05).Compared with doxorubicin group , the expression of calpain 1 decreased in doxo-rubicin+verapamil group (P<0.05).Compared with control group , the expression of LC3 increased in doxorubicin group and verapamil group (P<0.05).Compared with doxorubicin group , the expression of LC3 increased in doxorubicin +ver-apamil group ( P<0.01) .CONCLUSION:The drug resistance of TPC-1 cells to doxorubicin may be related to the in-crease in autophagic activity .Verapamil further increases autophagic activity of TPC-1 cells, resulting in autophagic death and inversing the resistance of TPC-1 cells to doxorubicin .The mechanism may be involved in L-Ca2+/calpain 1 signal transduction pathway of autophagy .

Objective To discuss the imaging features of pulmonary lophomonas blattarum infecfiom Methods Seventeen patients with renal homotransplantations presented fever without congh.dyspnea and shortness of breath in 1-4 months after the transplantation were included.Chest X-ray abnormalities were comfirmed as pulmonary lophomonag blattarum infection through fiberoptic bronchoscopy (FOB)and bronchoalveolar lavage(BAL).The X-ray and CT films were reviewed and the imaging features were summarized.Results X-ray appearances:Bilateral pulmonary shadows were seen in 16 patients.of which 9 cases presented patchy and cord shadows,and 7 cases presented large area of pathy shadows.Lung marking increase Wag seen in 1 case.CT appearances:CT abnormalities presented bilaterally and involved more than two lobes in all 16 patients.15 eases displayed ground-glass opacity.11 cases displayed patchy consolidation,14 cases displayed bandlike attenuation,and 8 cases displayed nodular opacity.Ground-glass opacity,consolidation and bandlike attenuation were seen simultaneously in 11 cases.Lymph node enlargement in mediastinum Wag presented in 10 cages.Conclusion Pulmonary lophomonas blattarum infection should be suspected in immunosuppressed patients combining the CT appearances and clinical information.