Objective To evaluate the effect of α-lipoic acid on the cognitive function after cardiopulmonary bypass (CPB) in diabetic rats.Methods Health adult male Sprague-Dawley rats,weighing 400-500 g,aged 16-22 weeks,were used in this study.Diabetes mellitus was induced by intraperitoneal streptozocin 60 mg/kg and confirmed by blood glucose≥ 16.7 mmol/L.Thirty-two diabetic rats were randomly divided into 2 groups (n =16 each):diabetes mellitus group (group D) and α-lipoi cacid group (group L).In group L,α-lipoic acid 30 mg/kg was intraperitoneally injected once a day for 7 consecutive days starting from 6th week after induction of diabetes mellitus.While the equal volume of normal saline was given instead in group D.The two groups underwent CPB after the last administration.Before induction of diabetes mellitus,on 5th week after induction of diabetes mellitus,before CPB,at the end of CPB,and on 3 and 5 days after termination of CPB,10 rats were chosen from each group and venous blood samples were collected for determination of plasma TNF-α and IL-10 concentrations.Ten rats in each group were chosen for detection of cognitive function before induction of diabetes mellitus,before CPB and 5 days after termination of CPB.The rats were then sacrificed and hippocampi were isolated for measurement of NF-κB activity.Results Compare with group D,the plasma TNF-α concentration,times of electric shock and activity of NF-κB in hippocampal tissues were significantly decreased and the plasma IL-10 concentration was increased in group L (P ＜ 0.05 or 0.01).Conclusion α-lipoic acid can improve the cognitive function after CPB in diabetic rats and inhibition of activation of NF-κB in hippocampal neurons is involved in the mechanism.

OBJECTIVETo preliminarily study the effect of fertilizer methods on the yield and quality of Fritillaria ussuriensis.

METHODThrough the investigation of the available nitrogen, phosphorus, potassium in soil fertility of F. ussuriensis planting field as well as the measurement of total nitrogen, phosphorus, potassium contents in bulbus of F. ussuriensis, the balanced fertilization plan for cultivation of F. ussuriensis was made.

RESULTThe optimal fertilizing amount was 13.0 kg x m(-2) pig dung, or 5.5 kg x m(-2) deer dung and 6.5 kg x m(-2) pig dung, or 11.0 kg x m(-2) deer dung.

CONCLUSIONEffective fertilizing may increase significantly the yield and quality of F. ussuriensis.

Agriculture ; methods ; Biomass ; Fertilizers ; analysis ; Fritillaria ; growth & development ; Soil ; analysis

Objective To retrospectively review the results of intraperitoneal placement of composite mesh for the repair of ventral incisional hernia. Methods Ten patients, in which the peritoneum was difficult to close direcdy, underwent repair of ventral incisional hernia with composite mesh between November 2003 and June 2005. The mesh was placed intraperitoneally. The mean duration of follow-up was 18 months (range 6 to 24). Results All the patients (10/10) were cured without postoperative complications such as wound infection, subcutaneous seroma and hematoma. During the follow-up study, no recurrences was observed. There were no long-term complications, such as bowel obstruction, fistula formation and fistulization to the small bowel. All the 10 patients felt comfortable. Conclusions Intraperitoneal placement of composite mesh is a safe and effective repair method for ventral incisional hernia.

Objective To investigate the key steps of hepatic artery reconstruction and the factors influencing the outcome of hepatic artery reconstruction in orthotopic liver transplantation. Methods The clinical data of 107 consecutive orthotopic liver transplantation patients was retrospectively reviewed to assess the key steps in hepatic artery reconstruction. The risk factors and the diagnosis and treatment of vascular complications were also discussed. Results The incidence of hepatic artery related complications in orthotopic liver transplantation was associated with the quality of the donor hepatic artery,the method used for anastomosis and the use of microsurgical technique. The main hepatic artery related complications were hepatic artery thrombosis and stenosis. The incidence of the vascular complications was 6.54%,and the mortality rate was 85.7%. Conclusion The major influence factors of vascular complications were the quality of the donor artery,the reconstruction way of donor-recipient artery and the use of microsurgical technique .

Objective To evaluate the effect of paraesophagogastric devascularization and splenectomy with transabdominal esophageal transection and reanastomosis in the treatment of portal hypertension Methods Clinical data of 48 portal hypertensive patients undergoing this procedure from June 1997 to June 2002 were analyzed retrospectively to evaluate the effect and complications of the operation Results Forty three patients were followed up for an average of 39 months The varicose veins eliminating rate was 93% The rebleeding rate was 2% The survival rate was 93% Two patients died of severe intra abdominal infection due to leakage of esophageal anastomosis and hepatic failure respectively during the early postoperative period Postoperative thrombosis rate in portal system was 8%, hydrops rate in splenic recess was 6%, the leakage and stenosis rate of anastomosis were 8% and 6% respectively Conclusion Paraesophagogastric devascularization and splenectomy with transabdominal esophageal transection is an effective method for the treatment of portal hypertension Because there is a risk of postoperative anastomosis leakage and stenosis, attention should be paid to the choice of patients for operation and manipulations during the operation

Objective To preliminarily develop a scale to measure the proactive socialization behavior of new nurses and test validity and reliability of the scale, to explore the impact of proactive socialization behavior on turnover intention. Methods Using a questionnaire survey method to investigate 696 new nurses who had worked within three years in nursing organization, structural equation model and hierarchical line regression methods were performed to analyze the data. Results Proactive socialization behavior scale included eight items under one dimension;the consistency coefficient of scale was 0.858;new scale had a good convergent and predictive validity;the proactive socialization behavior of new nurses was significantly negative related to turnover intention (β=-0.236,P<0.01). Conclusions The proactive socialization behavior scale has a good reliability, convergent and predictive validity. Meanwhile, new nurses with higher level of proactive socialization behavior have lower turnover intention. Because they can quickly fit the new environment, role definition and organizational culture, which in turn organization embeddedness is promoted.

OBJECTIVETo study the effects of Fe, Zn, B and Mn fertilizer with different ratio on the yield and quality of Aconitum carmichaeli.

METHODField experiment with the uniform design was applied, the yield and the contents of the total alkaloids and diester-alkaloids were measured.

RESULTFe, Zn, B and Mn fertilizer of appropriate ratio could promote the growth of vegetative organs, increase the biomass, the content of alkaloids and the yield of Aconite significantly. Fe, Zn fertilizer of highly concentrated ratio increased the proportion of first sub-roots, but inhibited the growth of other vegetative organs, the number of roots was less than that with other treatments, so it was not conducive to the formation of production. High concentration of Mn was not conducive to the growth of underground of Aconite, its number of sub-roots was fewer, but the number of third sub-roots was more than that with other treatments, the yield was low. The yield treated with low concentration of B was 10% higher than that with high concentration, and the high concentration of B was not conducive to increase the content of the alkaloids. Among these treatments, The fourth treatment was the optimal combination, of which the volume of sub-roots was the largest and the most homogeneous, the growth of the vegetative organs was better and the accumulation of dry matters was more, the yield of this treatment was 10,754.7 kg x hm(-2), which was increased by 14.9%, and the content of alkaloid was increased by 13.9%.

CONCLUSIONThe ratio of 4 is the best treatment for high yield and quality cultivation of Aconite.

Aconitum ; growth & development ; metabolism ; Alkaloids ; metabolism ; Biomass ; Fertilizers ; analysis ; Micronutrients ; metabolism ; Plant Roots ; growth & development

OBJECTIVETo study the effect of different seminal rhizomes on the growth, quality and quantity of Curcuma longa root.

METHODSingle factor randomized block design was applied, plant samples were collected and investigated periodically, and dry weight, production and the main active ingredient content were measured.

RESULTThe difference seminal rhizomes affected the growth, quality and quantity of C. longa root.

CONCLUSIONThe bigger and stronger rhizomes should be chosen as seeds.

Biomass ; Curcuma ; growth & development ; Plant Roots ; growth & development ; Quality Control ; Rhizome ; growth & development

OBJECTIVETo study the toxicity of water extracts from the fruits of Evodia Fructus in different producing areas.

METHODCompare the toxicity of the extracts from different Evodia Fructus on mice by the methods of acute and subacute toxicity test. The mice were given the extracts for 1 d to test the maximal tolerance dose (MTD) or maximal dose and observe the acute toxic symptoms; The mice were given the extracts for 15 d and then detected the level of serum alanine aminotransferase (ALT), aspartate aminotransferase (AST) and triglyceride (TG). The liver index was calculated, and the liver histological changes were investigated.

RESULTThe MTD of water extracts from the fruits of Evodia Fructus is 62, 44.8, 35.84 g x kg(-1); the MTD of Evodia Fructus is 56, 44. 8, 35.84 g x kg(-1); the maximal dose of Evodia Fructus is 60, 54, 45 g x kg(-1). The toxic symptoms of the mice which had been given the nine samples were almost consistent. Compared with the control group in subacute toxicity test, the level of serum ALT and the liver index were all increased. The liver histological were changed.

CONCLUSIONWhen water extracts from the fruits of Evodia Fructus are given to mice one or more times. It may be toxic and induce liver damage. There is no significant correlation between the toxicity and Evodia orgins, while the toxicity seems to be more closely related to the producing area.

Alanine Transaminase ; blood ; Animals ; Aspartate Aminotransferases ; blood ; China ; Drugs, Chinese Herbal ; administration & dosage ; metabolism ; toxicity ; Evodia ; chemistry ; Female ; Fruit ; chemistry ; Liver ; drug effects ; metabolism ; Male ; Mice ; Triglycerides ; blood