Objective:To investigate the relationship between ultrasonic shear wave elastic imaging, blood flow quantitative parameters and expression levels of GATA binding protein 3 (GATA3) and CD34 in breast cancer tissues.Methods:Ultrasound shear wave elastic imaging and blood flow parameter examination were performed with Resona R9 full-digital high-end color Doppler ultrasound diagnostic instrument of Shenzhen Mindray Biomedical Electronics Co., LTD., and maximum elasticity values (Emax), mean elasticity (Emean), minimum elasticity (Emin), pulsatility index (PI), and resistance index (RI) were recorded. The expression levels of GATA3 and CD34 in breast cancer tissues and adjacent tissues were detected by immunohistochemistry.Results:Compared with adjacent normal tissues, the proportion of low expression of GATA3 in breast cancer tissues was significantly increased, the difference was statistically significant ( χ2=9.673, P=0.002), and the proportion of high expression of CD34 was significantly increased, the difference was statistically significant ( χ2=37.104, P<0.001). The Emax, Emin, PI and RI in breast cancer patients with high GATA3 expression were significantly lower than those with low GATA3 expression, and the difference was statistically significant ( t was 4.136, 4.005, 5.387 and 4.103, respectively, P<0.05). The Emax, Emean, Emin, PI and RI of breast cancer patients with high expression of CD34 were significantly higher than those with low expression, with statistical significance ( t was 4.553, 4.037, 5.491, 3.894 and 5.273, respectively, P<0.05). Multivariate binary Logistic regression analysis showed that Emax, PI and RI were independent risk factors for low expression of GATA3 in breast cancer tissues, and the difference was statistically significant ( P<0.05). Emax, Emean, PI and RI were independent risk factors for high expression of CD34 in breast cancer tissues. The difference was statistically significant ( P<0.05). The sensitivity and specificity of ultrasound shear wave elastography combined with blood flow quantitative parameters were 78.7% and 81.7% for evaluating the low expression of GATA3 in breast cancer tissue, and 81.5% and 76.6% for evaluating the high expression of CD34 in breast cancer tissue, respectively. Conclusion:Ultrasonic shear wave elastic imaging and blood flow quantitative parameters are related to the expression levels of GATA3 and CD34 in breast cancer tissue, and have certain evaluation value.